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Basic Characteristics of Mutations
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Mutation Site
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T131I |
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Mutation Site Sentence
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Replicative competence of the T131I, K141E, and G145R surface variants of hepatitis B Virus. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
|
S |
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Standardized Encoding Gene
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S
|
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Genotype/Subtype
|
C |
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Viral Reference
|
-
|
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Functional Impact and Mechanisms
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Disease
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Hepatitis B Virus Infection
|
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Immune
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- |
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Target Gene
|
-
|
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Clinical and Epidemiological Correlations
|
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Clinical Information
|
- |
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Treatment
|
- |
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Location
|
- |
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Literature Information
|
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PMID
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17763322
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Title
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Replicative competence of the T131I, K141E, and G145R surface variants of hepatitis B Virus
|
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Author
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Jammeh S,Thomas HC,Karayiannis P
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Journal
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The Journal of infectious diseases
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Journal Info
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2007 Oct 1;196(7):1010-3
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Abstract
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Variants of hepatitis B surface antigen have been described in different clinical settings, but their replicative capacity in vitro has remained unexplored. Point mutations leading to sT131I, sK141E, and sG145R amino-acid substitutions were engineered by site-directed mutagenesis into an infectious plasmid clone of the virus. The mutated constructs were transfected into Huh7 cells, and their replication capacity was documented by LightCycler (Roche Diagnostics) measurements of virion-associated hepatitis B virus (HBV) DNA, intracellular relaxed circular double-stranded DNA, and pregenomic RNA. The sT131I and sG145R variants replicated with efficiency equal to that of the wild type, whereas the sK141E variant was replication impaired.
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Sequence Data
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-
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