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Basic Characteristics of Mutations
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Mutation Site
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T36G |
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Mutation Site Sentence
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A total of four nucleotide mismatches (T36G, A299G, T432C, and C666T) between pcDNA3-Y100C and pcDNA3-SA1 was found along the 681-bp fragment of S gene (not shown). |
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Mutation Level
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Nucleotide level |
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Mutation Type
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|
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Gene/Protein/Region
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PreS |
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Standardized Encoding Gene
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S
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Genotype/Subtype
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A1 |
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Viral Reference
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-
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Functional Impact and Mechanisms
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Disease
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-
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Immune
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- |
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Target Gene
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-
|
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Clinical and Epidemiological Correlations
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Clinical Information
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- |
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Treatment
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- |
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Location
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- |
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Literature Information
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PMID
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21331286
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Title
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Expression of Hepatitis B Virus Surface Antigen Containing Y100C Variant Frequently Detected in Occult HBV Infection
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Author
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Mello FC,Martel N,Gomes SA,Araujo NM
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Journal
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Hepatitis research and treatment
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Journal Info
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2011;2011:695859
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Abstract
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Small hepatitis B virus surface protein (S-HBsAg) variant Y100C has been associated with HBsAg-negative phenotype. To determine whether Y100C substitution yields impaired HBsAg or small amounts of HBsAg that may reduce HBsAg detection by commercial anti-HBsAg antibodies, two eukaryotic expression plasmids, one containing a wild-type S and the other an S gene from a Y100C variant, were constructed and their levels of HBsAg compared by ELISA after transfection of HuH7 cells. Unexpectedly, the extracellular HBsAg levels detected with Y100C plasmid were higher than those observed with the wild-type plasmid, but without statistical significance. We concluded that the Y100C substitution alone did not play a role in reducing HBsAg amounts or HBsAg affinity by commercial ELISA assay. Further studies on in vitro replication fitness with the complete genome of HBV isolates displaying or not Y100C substitution may elucidate whether this mutation affects HBV replication and consequently HBsAg production.
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Sequence Data
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HQ840709;EF690524
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