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Basic Characteristics of Mutations
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Mutation Site
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T62M |
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Mutation Site Sentence
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Within the NS5A gene, the T62M mutation was detected in 2 individuals. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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NS5A |
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Standardized Encoding Gene
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NS5A
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Genotype/Subtype
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5 |
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Viral Reference
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-
|
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Functional Impact and Mechanisms
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Disease
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HCV Infection
|
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Immune
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- |
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Target Gene
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-
|
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Clinical and Epidemiological Correlations
|
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Clinical Information
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Y |
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Treatment
|
- |
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Location
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South Africa |
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Literature Information
|
|
PMID
|
37231989
|
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Title
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Hepatitis C Virus Genotype 5 Variability in Treatment-Naive Patients in South Africa
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Author
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Maunye TK,Gededzha MP,Blackard JT,Rakgole JN,Selabe SG
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Journal
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Intervirology
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Journal Info
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2023;66(1):77-87
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Abstract
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INTRODUCTION: Hepatitis C virus (HCV) genotype 5 was originally identified in South Africa, where it represents 35-60% of all HCV infections. There are limited data on resistance-associated variants (RAVs) in South Africa. Thus, we investigated variability within the NS3/NS4A, NS5A, and NS5B genes of treatment-naive individuals with HCV genotype 5 infection at the Dr. George Mukhari Academic Hospital (DGMAH) in Pretoria, South Africa. METHODS: Nested PCR was performed to amplify the NS3/4A, NS5A, and NS5B genes. RAVs were evaluated using the Geno2pheno tool. RESULTS: In the NS3/4A gene, F56S and T122A were detected in one sample each. The D168E mutation was detected in 7 samples. Within the NS5A gene, the T62M mutation was detected in 2 individuals. In the NS5B gene, 8 of 12 individuals (67%) had the A421V mutation, while all 12 individuals (100%) had the S486A mutation. DISCUSSION: RAVs were detected frequently among treatment-naive individuals with HCV genotype 5 infection in South Africa. Thus, resistance testing may be prudent when initiating treatment of patients with genotype 5 infection. Additional population-based studies are needed to understand the prevalence of these RAVs during HCV genotype 5 infection.
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Sequence Data
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KC767829–KC767834;ON228285–ON228302
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