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Basic Characteristics of Mutations
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Mutation Site
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V11G |
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Mutation Site Sentence
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To confirm that this loss of function was not particular to HIV subtype B, we introduced S8R or I/V11G substitutions into selected subtype A, C and D Nef clones that displayed normal abilities to internalize SERINC3. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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Nef |
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Standardized Encoding Gene
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Nef
|
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Genotype/Subtype
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HIV-1 |
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Viral Reference
|
-
|
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Functional Impact and Mechanisms
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Disease
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HIV Infections
|
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Immune
|
- |
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Target Gene
|
-
|
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Clinical and Epidemiological Correlations
|
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Clinical Information
|
- |
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Treatment
|
Nef |
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Location
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Uganda |
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Literature Information
|
|
PMID
|
32925973
|
|
Title
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Variation in HIV-1 Nef function within and among viral subtypes reveals genetically separable antagonism of SERINC3 and SERINC5
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Author
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Jin SW,Mwimanzi FM,Mann JK,Bwana MB,Lee GQ,Brumme CJ,Hunt PW,Martin JN,Bangsberg DR,Ndung'u T,Brumme ZL,Brockman MA
|
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Journal
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PLoS pathogens
|
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Journal Info
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2020 Sep 14;16(9):e1008813
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Abstract
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HIV Nef counteracts cellular host restriction factors SERINC3 and SERINC5, but our understanding of how naturally occurring global Nef sequence diversity impacts these activities is limited. Here, we quantify SERINC3 and SERINC5 internalization function for 339 Nef clones, representing the major pandemic HIV-1 group M subtypes A, B, C and D. We describe distinct subtype-associated hierarchies for Nef-mediated internalization of SERINC5, for which subtype B clones display the highest activities on average, and of SERINC3, for which subtype B clones display the lowest activities on average. We further identify Nef polymorphisms that modulate its ability to counteract SERINC proteins, including substitutions in the N-terminal domain that selectively impair SERINC3 internalization. Our findings demonstrate that the SERINC antagonism activities of HIV Nef differ markedly among major viral subtypes and between individual isolates within a subtype, suggesting that variation in these functions may contribute to global differences in viral pathogenesis.
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Sequence Data
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KC906733-KC907077
|
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