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Basic Characteristics of Mutations
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Mutation Site
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V59F |
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Mutation Site Sentence
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SDAC031 (D6) showed preS2I42T, spV59F and rtT237P. |
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Mutation Level
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Amino acid level |
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Mutation Type
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Nonsynonymous substitution |
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Gene/Protein/Region
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P |
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Standardized Encoding Gene
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P
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Genotype/Subtype
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D;E |
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Viral Reference
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AB205191.1;GU456684.1
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Functional Impact and Mechanisms
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Disease
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Liver Diseases
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Immune
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- |
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Target Gene
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-
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Clinical and Epidemiological Correlations
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Clinical Information
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Y |
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Treatment
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- |
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Location
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Sudan |
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Literature Information
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PMID
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23865777
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Title
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Molecular characterization of hepatitis B virus in liver disease patients and asymptomatic carriers of the virus in Sudan
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Author
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Yousif M,Mudawi H,Bakhiet S,Glebe D,Kramvis A
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Journal
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BMC infectious diseases
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Journal Info
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2013 Jul 18;13:328
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Abstract
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BACKGROUND: Hepatitis B virus is hyperendemic in Sudan. Our aim was to molecularly characterize hepatitis B virus from Sudanese individuals, with and without liver disease, because genotypes play an important role in clinical manifestation and treatment management. METHODS: Ninety-nine patients - 30 asymptomatic, 42 cirrhotic, 15 with hepatocellular carcinoma, 7 with acute hepatitis and 5 with chronic hepatitis- were enrolled. Sequencing of surface and basic core promoter/precore regions and complete genome were performed. RESULTS: The mean +/- standard deviation, age was 45.7+/-14.8 years and the male to female ratio 77:22. The median (interquartile range) of hepatitis B virus DNA and alanine aminotransferase levels were 2.8 (2.2-4.2) log IU/ml and 30 (19-49) IU/L, respectively. Using three genotyping methods, 81/99 (82%) could be genotyped. Forty eight percent of the 99 patients were infected with genotype D and 24% with genotype E, 2% with putative D/E recombinants and 7% with genotype A. Patients infected with genotype E had higher frequency of hepatitis B e antigen-positivity and higher viral loads compared to patients infected with genotype D. Basic core promoter/precore region mutations, including the G1896A in 37% of HBeAg-negative individuals, could account for hepatitis B e antigen-negativity. Pre-S deletion mutants were found in genotypes D and E. Three isolates had the vaccine escape mutant sM133T. CONCLUSION: Sudanese hepatitis B virus carriers were mainly infected with genotypes D or E, with patients infected with genotype E having higher HBeAg-positivity and higher viral loads. This is the first study to molecularly characterize hepatitis B virus from liver disease patients in Sudan.
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Sequence Data
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KF170739-KF170812
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