SARS-CoV-2 Mutation Detail Information

Virus Mutation SARS-CoV-2 Mutation V987P


Basic Characteristics of Mutations
Mutation Site V987P
Mutation Site Sentence BNT162b2 encodes the full-length transmembrane S glycoprotein,locked in its prefusion conformation by the substitution of two residues with proline (S(K986P/V987P);hereafter,S(P2) (also known as P2 S)).
Mutation Level Amino acid level
Mutation Type Nonsynonymous substitution
Gene/Protein/Region S
Standardized Encoding Gene S  
Genotype/Subtype -
Viral Reference -
Functional Impact and Mechanisms
Disease Cell line    
Immune -
Target Gene -
Clinical and Epidemiological Correlations
Clinical Information -
Treatment -
Location -
Literature Information
PMID 33524990
Title BNT162b vaccines protect rhesus macaques from SARS-CoV-2
Author Vogel AB,Kanevsky I,Che Y,Swanson KA,Muik A,Vormehr M,Kranz LM,Walzer KC,Hein S,Guler A,Loschko J,Maddur MS,Ota-Setlik A,Tompkins K,Cole J,Lui BG,Ziegenhals T,Plaschke A,Eisel D,Dany SC,Fesser S,Erbar S,Bates F,Schneider D,Jesionek B,Sanger B,Wallisch AK,Feuchter Y,Junginger H,Krumm SA,Heinen AP,Adams-Quack P,Schlereth J,Schille S,Kroner C,de la Caridad Guimil Garcia R,Hiller T,Fischer L,Sellers RS,Choudhary S,Gonzalez O,Vascotto F,Gutman MR,Fontenot JA,Hall-Ursone S,Brasky K,Griffor MC,Han S,Su AAH,Lees JA,Nedoma NL,Mashalidis EH,Sahasrabudhe PV,Tan CY,Pavliakova D,Singh G,Fontes-Garfias C,Pride M,Scully IL,Ciolino T,Obregon J,Gazi M,Carrion R Jr,Alfson KJ,Kalina WV,Kaushal D,Shi PY,Klamp T,Rosenbaum C,Kuhn AN,Tureci O,Dormitzer PR,Jansen KU,Sahin U
Journal Nature
Journal Info 2021 Apr;592(7853):283-289
Abstract A safe and effective vaccine against COVID-19 is urgently needed in quantities that are sufficient to immunize large populations. Here we report the preclinical development of two vaccine candidates (BNT162b1 and BNT162b2) that contain nucleoside-modified messenger RNA that encodes immunogens derived from the spike glycoprotein (S) of SARS-CoV-2, formulated in lipid nanoparticles. BNT162b1 encodes a soluble, secreted trimerized receptor-binding domain (known as the RBD-foldon). BNT162b2 encodes the full-length transmembrane S glycoprotein, locked in its prefusion conformation by the substitution of two residues with proline (S(K986P/V987P); hereafter, S(P2) (also known as P2 S)). The flexibly tethered RBDs of the RBD-foldon bind to human ACE2 with high avidity. Approximately 20% of the S(P2) trimers are in the two-RBD 'down', one-RBD 'up' state. In mice, one intramuscular dose of either candidate vaccine elicits a dose-dependent antibody response with high virus-entry inhibition titres and strong T-helper-1 CD4(+) and IFNgamma(+)CD8(+) T cell responses. Prime-boost vaccination of rhesus macaques (Macaca mulatta) with the BNT162b candidates elicits SARS-CoV-2-neutralizing geometric mean titres that are 8.2-18.2x that of a panel of SARS-CoV-2-convalescent human sera. The vaccine candidates protect macaques against challenge with SARS-CoV-2; in particular, BNT162b2 protects the lower respiratory tract against the presence of viral RNA and shows no evidence of disease enhancement. Both candidates are being evaluated in phase I trials in Germany and the USA(1-3), and BNT162b2 is being evaluated in an ongoing global phase II/III trial (NCT04380701 and NCT04368728).
Sequence Data -
Mutation Information
Note
Basic Characteristics of Mutations
  • Mutation Site: The specific location in a gene or protein sequence where a change occurs.
  • Mutation Level: The level at which a mutation occurs, including the nucleotide or amino acid level.
  • Mutation Type: The nature of the mutation, such as missense mutation, nonsense mutation, synonymous mutation, etc.
  • Gene/Protein/Region: Refers to the specific region of the virus where the mutation occurs. Including viral genes, viral proteins, or a specific viral genome region. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main
  • Gene/Protein/Region studied in the article is marked.
  • Genotype/Subtype: Refers to the viral genotype or subtype where the mutation occurs. If the article does not specifically indicate the relationship between the mutation and its correspondence, the main Genotype/Subtype studied in the article is marked.
  • Viral Reference: Refers to the standard virus strain used to compare and analyze viral sequences.
Functional Impact and Mechanisms
  • Disease: An abnormal physiological state with specific symptoms and signs caused by viral infection.
  • Immune: The article focuses on the study of mutations and immune.
  • Target Gene: Host genes that viral mutations may affect.
Clinical and Epidemiological Correlations
  • Clinical Information: The study is a clinical or epidemiological study and provides basic information about the population.
  • Treatment: The study mentioned a certain treatment method, such as drug resistance caused by mutations. If the study does not specifically indicate the relationship between mutations and their correspondence treatment, the main treatment studied in the article is marked.
  • Location: The source of the research data.
Literature Information
  • Sequence Data: The study provides the data accession number.