Accession	GSE100044
Status	2017/6/15
Title	Identification of symmetric methylation of histone H4 arginine 3 on KSHV genome (H4R3me2s ChIP-Seq)
Organism	Human gammaherpesvirus 8
Experiment type	Genome binding/occupancy profiling by high throughput sequencing
Summary	The objective of this study was to identify the symmetric methylation sites on KSHV genome during latency and lytic reactivation
Samples	GSM ID Sample info Characteristics Description GSM2668641 Und-Input tissue:KSHV infected Body Cavity Based Lymphoma Cells; treatment:RTA protein is under tet-inducible promoter; antibody:none (input) DNA extracted from the sonicated chromatin GSM2668642 Und-IgG ChIP tissue:KSHV infected Body Cavity Based Lymphoma Cells; treatment:RTA protein is under tet-inducible promoter; antibody:Control IgG Antibody (Cell Signaling Technology #2729) Control IgG ChIP DNA GSM2668643 Und-H4 ChIP tissue:KSHV infected Body Cavity Based Lymphoma Cells; treatment:RTA protein is under tet-inducible promoter; antibody:Histone H4 Antibody (Active Motif #61299) Histone H4 ChIP DNA GSM2668644 Und-H4R3me2sym ChIP tissue:KSHV infected Body Cavity Based Lymphoma Cells; treatment:RTA protein is under tet-inducible promoter; antibody:Symmetric di-methylated histone H4 (H4R3me2s) Antibody (Abcam # ab5823) H4R3sym ChIP DNA GSM2668645 12hInd-Input tissue:KSHV infected Body Cavity Based Lymphoma Cells; treatment:RTA protein is under tet-inducible promoter; antibody:none (input) DNA extracted from the sonicated chromatin GSM2668646 12hInd-IgG ChIP tissue:KSHV infected Body Cavity Based Lymphoma Cells; treatment:RTA protein is under tet-inducible promoter; antibody:Control IgG Antibody (Cell Signaling Technology #2729) Control IgG ChIP DNA GSM2668647 12hInd-H4 ChIP tissue:KSHV infected Body Cavity Based Lymphoma Cells; treatment:RTA protein is under tet-inducible promoter; antibody:Histone H4 Antibody (Active Motif #61299) Histone H4 ChIP DNA GSM2668648 12hInd-H4R3me2sym ChIP tissue:KSHV infected Body Cavity Based Lymphoma Cells; treatment:RTA protein is under tet-inducible promoter; antibody:Symmetric di-methylated histone H4 (H4R3me2s) Antibody (Abcam # ab5823) H4R3sym ChIP DNA
Platform	GPL23366  : Illumina NextSeq 500 (Human gammaherpesvirus 8)
Literature	28678843