Accession	GSE104621
Status	2018/3/29
Title	N6-methyladenosine modification in lytic viral gene expression during Kaposi's sarcoma-associated herpesvirus infection [MeRIP-seq]
Organism	Homo sapiens
Experiment type	Methylation profiling by high throughput sequencing
Summary	Methylation at the N6 position of adenosine (m6A) is a highly prevalent reversible modification within eukaryotic mRNAs that has been linked to many stages of RNA processing and fate. Recent studies suggest that m6A deposition and proteins involved in the m6A pathway play a diverse set of roles in either restricting or modulating the lifecycles of select viruses. Here, we report that m6A levels are significantly increased in cells infected with the oncogenic human DNA virus Kaposi’s sarcoma-associated herpesvirus (KSHV). Transcriptome-wide m6A-sequencing of the KSHV-positive renal carcinoma cell line iSLK.219 during lytic reactivation revealed the presence of m6A across multiple kinetic classes of viral transcripts, and a concomitant decrease in m6A levels across much of the host transcriptome.
Samples	GSM ID Sample info Characteristics Description GSM2804222 induced iSLK.219 cells input rep 1 cell line:iSLK.219; cell type:KSHV-positive renal carcinoma cell line; genotype/variation:containing doxycycline-inducible KSHV.219; passages:8; induced with:doxycyclin for 5 days; rip antibody:none; molecule subtype:Total RNA processed data file: human_Union_induced_FC4_genes.txt GSM2804223 uninduced iSLK.219 cells m6A IP rep 2 cell line:iSLK.219; cell type:KSHV-positive renal carcinoma cell line; genotype/variation:containing doxycycline-inducible KSHV.219; passages:8; induced with:none (uninduced); rip antibody:anti-m6A (Synaptic Systems); molecule subtype:m6A-modified RNA processed data file: human_Union_uninduced_FC4_genes.txt GSM2804224 uninduced iSLK.219 cells input rep 2 cell line:iSLK.219; cell type:KSHV-positive renal carcinoma cell line; genotype/variation:containing doxycycline-inducible KSHV.219; passages:8; induced with:none (uninduced); rip antibody:none; molecule subtype:Total RNA processed data file: human_Union_uninduced_FC4_genes.txt GSM2804225 induced iSLK.219 cells m6A IP rep 2 cell line:iSLK.219; cell type:KSHV-positive renal carcinoma cell line; genotype/variation:containing doxycycline-inducible KSHV.219; passages:8; induced with:doxycyclin for 5 days; rip antibody:anti-m6A (Synaptic Systems); molecule subtype:m6A-modified RNA processed data file: human_Union_induced_FC4_genes.txt GSM2804226 induced iSLK.219 cells input rep 2 cell line:iSLK.219; cell type:KSHV-positive renal carcinoma cell line; genotype/variation:containing doxycycline-inducible KSHV.219; passages:8; induced with:doxycyclin for 5 days; rip antibody:none; molecule subtype:Total RNA processed data file: human_Union_induced_FC4_genes.txt GSM2804219 uninduced iSLK.219 cells m6A IP rep 1 cell line:iSLK.219; cell type:KSHV-positive renal carcinoma cell line; genotype/variation:containing doxycycline-inducible KSHV.219; passages:8; induced with:none (uninduced); rip antibody:anti-m6A (Synaptic Systems); molecule subtype:m6A-modified RNA processed data file: human_Union_uninduced_FC4_genes.txt GSM2804220 uninduced iSLK.219 cells input rep 1 cell line:iSLK.219; cell type:KSHV-positive renal carcinoma cell line; genotype/variation:containing doxycycline-inducible KSHV.219; passages:8; induced with:none (uninduced); rip antibody:none; molecule subtype:Total RNA processed data file: human_Union_uninduced_FC4_genes.txt GSM2804221 induced iSLK.219 cells m6A IP rep 1 cell line:iSLK.219; cell type:KSHV-positive renal carcinoma cell line; genotype/variation:containing doxycycline-inducible KSHV.219; passages:8; induced with:doxycyclin for 5 days; rip antibody:anti-m6A (Synaptic Systems); molecule subtype:m6A-modified RNA processed data file: human_Union_induced_FC4_genes.txt
Platform	GPL16791  : Illumina HiSeq 2500 (Homo sapiens)
Literature	29659627