Accession	GSE124801
Status	2020/1/1
Title	The epigenetic landscapes of histone modifications on HSV-1 genome in human THP-1 cells [ChIP-seq]
Organism	Homo sapiens
Experiment type	Genome binding/occupancy profiling by high throughput sequencing
Summary	To study the dynamic changes of histone modifications across HSV-1 genome during lytic infection in THP-1 cells. Totally 20 maps of HSV-1 epigenome were generated at 5 different time points after infection, together with their corresponding gene expression profiles.We found that histone modifications were detected on HSV-1 genome soon after infection; all four studied modifications, H3K9me3, H3K27me3, H3K4me3 and H3K27ac, change rapidly along with virus life cycle progression.The transcription repression marks, H3K9me3 and H3K27me3, tended to decrease along with infection process, and the transcription activation mark H3K27ac increased on viral genome, which were aligned with increased expression of viral genes. Moreover, treatment with C646, an inhibitor for H3K27ac transferase p300, inhibited expression of viral genes; and EPZ6438, an inhibitor for H3K27 methyltransferase EZH2, enhanced viral gene expression.
Samples	GSM ID Sample info Characteristics Description GSM3554218 H3K27AC_HSV-1_1H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 1h; chip antibody:H3K27ac (Abcam Ab4729) processed data file:;HSV-H3K27ac-1h.bw GSM3554219 H3K27AC_HSV-1_1H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 1h; chip antibody:H3K27ac (Abcam Ab4729) processed data file:;HSV-H3K27ac-1h.bw GSM3554220 H3K27AC_HSV-1_2H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 2h; chip antibody:H3K27ac (Abcam Ab4729) processed data file:;HSV-H3K27ac-2h.bw GSM3554221 H3K27AC_HSV-1_2H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 2h; chip antibody:H3K27ac (Abcam Ab4729) processed data file:;HSV-H3K27ac-2h.bw GSM3554222 H3K27AC_HSV-1_4H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 4h; chip antibody:H3K27ac (Abcam Ab4729) processed data file:;HSV-H3K27ac-4h.bw GSM3554223 H3K27AC_HSV-1_4H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 4h; chip antibody:H3K27ac (Abcam Ab4729) processed data file:;HSV-H3K27ac-4h.bw GSM3554230 H3K27ME3_HSV-1_2H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 2h; chip antibody:H3K27me3 (clone C36B11, CST 9733) processed data file:;HSV-H3K27me3-2h.bw GSM3554231 H3K27ME3_HSV-1_2H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 2h; chip antibody:H3K27me3 (clone C36B11, CST 9733) processed data file:;HSV-H3K27me3-2h.bw GSM3554232 H3K27ME3_HSV-1_4H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 4h; chip antibody:H3K27me3 (clone C36B11, CST 9733) processed data file:;HSV-H3K27me3-4h.bw GSM3554233 H3K27ME3_HSV-1_4H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 4h; chip antibody:H3K27me3 (clone C36B11, CST 9733) processed data file:;HSV-H3K27me3-4h.bw GSM3554224 H3K27AC_HSV-1_8H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 8h; chip antibody:H3K27ac (Abcam Ab4729) processed data file:;HSV-H3K27ac-8h.bw GSM3554234 H3K27ME3_HSV-1_8H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 8h; chip antibody:H3K27me3 (clone C36B11, CST 9733) processed data file:;HSV-H3K27me3-8h.bw GSM3554235 H3K27ME3_HSV-1_8H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 8h; chip antibody:H3K27me3 (clone C36B11, CST 9733) processed data file:;HSV-H3K27me3-8h.bw GSM3554236 H3K27ME3_HSV-1_24H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 24h; chip antibody:H3K27me3 (clone C36B11, CST 9733) processed data file:;HSV-H3K27me3-24h.bw GSM3554237 H3K27ME3_HSV-1_24H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 24h; chip antibody:H3K27me3 (clone C36B11, CST 9733) processed data file:;HSV-H3K27me3-24h.bw GSM3554238 H3K4ME3_HSV-1_1H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 1h; chip antibody:H3K4me3 (clone MC315, Millipore 04-745) processed data file:;HSV-H3K4me3-1h.bw GSM3554225 H3K27AC_HSV-1_8H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 8h; chip antibody:H3K27ac (Abcam Ab4729) processed data file:;HSV-H3K27ac-8h.bw GSM3554226 H3K27AC_HSV-1_24H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 24h; chip antibody:H3K27ac (Abcam Ab4729) processed data file:;HSV-H3K27ac-24h.bw GSM3554227 H3K27AC_HSV-1_24H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 24h; chip antibody:H3K27ac (Abcam Ab4729) processed data file:;HSV-H3K27ac-24h.bw GSM3554228 H3K27ME3_HSV-1_1H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 1h; chip antibody:H3K27me3 (clone C36B11, CST 9733) processed data file:;HSV-H3K27me3-1h.bw GSM3554229 H3K27ME3_HSV-1_1H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 1h; chip antibody:H3K27me3 (clone C36B11, CST 9733) processed data file:;HSV-H3K27me3-1h.bw GSM3554239 H3K4ME3_HSV-1_1H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 1h; chip antibody:H3K4me3 (clone MC315, Millipore 04-745) processed data file:;HSV-H3K4me3-1h.bw GSM3554240 H3K4ME3_HSV-1_2H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 2h; chip antibody:H3K4me3 (clone MC315, Millipore 04-745) processed data file:;HSV-H3K4me3-2h.bw GSM3554241 H3K4ME3_HSV-1_2H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 2h; chip antibody:H3K4me3 (clone MC315, Millipore 04-745) processed data file:;HSV-H3K4me3-2h.bw GSM3554242 H3K4ME3_HSV-1_4H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 4h; chip antibody:H3K4me3 (clone MC315, Millipore 04-745) processed data file:;HSV-H3K4me3-4h.bw GSM3554243 H3K4ME3_HSV-1_4H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 4h; chip antibody:H3K4me3 (clone MC315, Millipore 04-745) processed data file:;HSV-H3K4me3-4h.bw GSM3554244 H3K4ME3_HSV-1_8H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 8h; chip antibody:H3K4me3 (clone MC315, Millipore 04-745) processed data file:;HSV-H3K4me3-8h.bw GSM3554245 H3K4ME3_HSV-1_8H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 8h; chip antibody:H3K4me3 (clone MC315, Millipore 04-745) processed data file:;HSV-H3K4me3-8h.bw GSM3554246 H3K4ME3_HSV-1_24H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 24h; chip antibody:H3K4me3 (clone MC315, Millipore 04-745) processed data file:;HSV-H3K4me3-24h.bw GSM3554247 H3K4ME3_HSV-1_24H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 24h; chip antibody:H3K4me3 (clone MC315, Millipore 04-745) processed data file:;HSV-H3K4me3-24h.bw GSM3554248 H3K9ME3_HSV-1_1H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 1h; chip antibody:H3K9me3 (Abcam ab176916) processed data file:;HSV-H3K9me3-1h.bw GSM3554249 H3K9ME3_HSV-1_1H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 1h; chip antibody:H3K9me3 (Abcam ab176916) processed data file:;HSV-H3K9me3-1h.bw GSM3554250 H3K9ME3_HSV-1_1H_ChIPSeq_rep3 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 1h; chip antibody:H3K9me3 (Abcam ab176916) processed data file:;HSV-H3K9me3-1h.bw GSM3554251 H3K9ME3_HSV-1_2H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 2h; chip antibody:H3K9me3 (Abcam ab176916) processed data file:;HSV-H3K9me3-2h.bw GSM3554252 H3K9ME3_HSV-1_2H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 2h; chip antibody:H3K9me3 (Abcam ab176916) processed data file:;HSV-H3K9me3-2h.bw GSM3554253 H3K9ME3_HSV-1_2H_ChIPSeq_rep3 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 2h; chip antibody:H3K9me3 (Abcam ab176916) processed data file:;HSV-H3K9me3-2h.bw GSM3554254 H3K9ME3_HSV-1_4H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 4h; chip antibody:H3K9me3 (Abcam ab176916) processed data file:;HSV-H3K9me3-4h.bw GSM3554255 H3K9ME3_HSV-1_4H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 4h; chip antibody:H3K9me3 (Abcam ab176916) processed data file:;HSV-H3K9me3-4h.bw GSM3554256 H3K9ME3_HSV-1_4H_ChIPSeq_rep3 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 4h; chip antibody:H3K9me3 (Abcam ab176916) processed data file:;HSV-H3K9me3-4h.bw GSM3554257 H3K9ME3_HSV-1_8H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 8h; chip antibody:H3K9me3 (Abcam ab176916) processed data file:;HSV-H3K9me3-8h.bw GSM3554258 H3K9ME3_HSV-1_8H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 8h; chip antibody:H3K9me3 (Abcam ab176916) processed data file:;HSV-H3K9me3-8h.bw GSM3554259 H3K9ME3_HSV-1_8H_ChIPSeq_rep3 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 8h; chip antibody:H3K9me3 (Abcam ab176916) processed data file:;HSV-H3K9me3-8h.bw GSM3554260 H3K9ME3_HSV-1_24H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 24h; chip antibody:H3K9me3 (Abcam ab176916) processed data file:;HSV-H3K9me3-24h.bw GSM3554261 H3K9ME3_HSV-1_24H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 24h; chip antibody:H3K9me3 (Abcam ab176916) processed data file:;HSV-H3K9me3-24h.bw GSM3554262 H3K9ME3_HSV-1_24H_ChIPSeq_rep3 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 24h; chip antibody:H3K9me3 (Abcam ab176916) processed data file:;HSV-H3K9me3-24h.bw GSM3554263 INPUT_HSV-1_1H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 1h; chip antibody:none - GSM3554264 INPUT_HSV-1_1H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 1h; chip antibody:none - GSM3554265 INPUT_HSV-1_1H_ChIPSeq_rep3 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 1h; chip antibody:none - GSM3554266 INPUT_HSV-1_2H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 2h; chip antibody:none - GSM3554267 INPUT_HSV-1_2H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 2h; chip antibody:none - GSM3554268 INPUT_HSV-1_2H_ChIPSeq_rep3 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 2h; chip antibody:none - GSM3554269 INPUT_HSV-1_4H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 4h; chip antibody:none - GSM3554270 INPUT_HSV-1_4H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 4h; chip antibody:none - GSM3554271 INPUT_HSV-1_4H_ChIPSeq_rep3 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 4h; chip antibody:none - GSM3554272 INPUT_HSV-1_8H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 8h; chip antibody:none - GSM3554273 INPUT_HSV-1_8H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 8h; chip antibody:none - GSM3554274 INPUT_HSV-1_8H_ChIPSeq_rep3 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 8h; chip antibody:none - GSM3554275 INPUT_HSV-1_24H_ChIPSeq_rep1 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 24h; chip antibody:none - GSM3554276 INPUT_HSV-1_24H_ChIPSeq_rep2 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 24h; chip antibody:none - GSM3554277 INPUT_HSV-1_24H_ChIPSeq_rep3 host cell line:THP-1; host cell type:monocyte; tissue:peripheral blood; disease:acute monocytic leukemia; treatment:HSV-1 infect 24h; chip antibody:none -
Platform	GPL26016  : HiSeq X Ten (Homo sapiens; Human alphaherpesvirus 1)
Literature	32014498