Accession	GSE124856
Status	2020/2/16
Title	Genome-wide maps of LSD1-RCOR2 binding in Merkel cell carcinoma cells
Organism	Homo sapiens
Experiment type	Genome binding/occupancy profiling by high throughput sequencing
Summary	Merkel cell carcinoma (MCC) is an aggressive neuroendocrine cancer of the skin, caused by either excessive UV damage or integration of the Merkel cell polyomavirus (MCV) genome. Here, we report that virally encoded MCV small T antigen (ST) establishes dependence on the LSD1 transcriptional repressor. Inhibition of LSD1 reduces growth of MCV-positive MCC and suppresses ST’s transformation capacity in vitro and in vivo. To define the mechanism of LSD1 inhibition in MCC, we performed a CRISPR loss-of-function library screen. We found that deletion of components of the non-canonical (ncBAF) chromatin remodeler complex confers resistance to LSD1 inhibitors and that LSD1 and ncBAF antagonistically regulate an overlapping set of genes involved in neuron differentiation. Our work provides mechanistic insight into the dependence of MCC on LSD1 and the role of ncBAF as a tumor suppressor in cancer.
Samples	GSM ID Sample info Characteristics Description GSM3557211 LSD1_1 tumor type:Merkel cell carcinoma; cell type:MCC tumor derived cell line; cell line:MKL-1; chip antibody:LSD1 (abcam; rabbit; ab17721) - GSM3557212 LSD1_2 tumor type:Merkel cell carcinoma; cell type:MCC tumor derived cell line; cell line:MKL-1; chip antibody:LSD1 (abcam; rabbit; ab17721) - GSM3557213 RCOR2_1 tumor type:Merkel cell carcinoma; cell type:MCC tumor derived cell line; cell line:MKL-1; chip antibody:RCOR2 (Proteintech; rabbit; 23969-1-AP) - GSM3557214 RCOR2_2 tumor type:Merkel cell carcinoma; cell type:MCC tumor derived cell line; cell line:MKL-1; chip antibody:RCOR2 (Proteintech; rabbit; 23969-1-AP) - GSM3557215 Input_1 tumor type:Merkel cell carcinoma; cell type:MCC tumor derived cell line; cell line:MKL-1; chip antibody:none - GSM3557216 Input_2 tumor type:Merkel cell carcinoma; cell type:MCC tumor derived cell line; cell line:MKL-1; chip antibody:none - GSM3613345 ATOH1_1 cell type:MCC tumor derived cell line; cell line:MKL-1; chip antibody:ATOH1 - GSM3613346 ATOH1_2 cell type:MCC tumor derived cell line; cell line:MKL-1; chip antibody:ATOH1 -
Platform	GPL18573  : Illumina NextSeq 500 (Homo sapiens)
Literature	32415271
Download	Download Genome Binding/Occupancy Profiling Analysis Data