Virus Dataset Detail

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Dataset Information
Accession GSE128121
Status 2020/3/31
Title Pharmacological Inhibition of PPARγ Reverses HIV Latency but Prevents Viral Production/Infectivity while Boosting Th17 Functions
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary In addition to HIV-permissiveness factors, Th17-cells express PPARγ, a transcriptional repressor of specific genes including RORγt and HIV. Here, we investigated the effect of PPARγ pharmacological inhibition on HIV replication and Th17 functions. As predicted, T-cell receptor triggering in the presence of the PPARG antagonist T0070907 increased IL-17A production and cell-associated HIV-RNA levels in T-cells of HIV-infected ART-treated individuals. Unexpectedly, T0070907 inhibited HIV-RNA release/viral outgrowth ex vivo and transmitted/founder HIV replication in vitro. Whole genome RNA-sequencing revealed multiple genes/pathways modulated by T0070907, including those involved in HIV-permissiveness (e.g., CCR5, furin) versus HIV-restriction (e.g., TRIM22, BST2, miR29), as well as Th17-effector functions (e.g., RORγt, STAT3, BCL6, IL-17A/F, IL-21, IL-22, IL-26). Of note, T0070907 boosted IL-21, a cytokine exhibiting immune-regulatory and miR-29-mediated antiviral properties. miR-29 antagomir failed to restore HIV replication in T0070907-treated T-cells, indicative of a pleiotropic antiviral program triggered by PPARG antagonism.
Samples
GSM ID Sample info Characteristics Description
GSM3665241 14: T007 tissue:PBMC sorted CCR6+ T-cells; barcode:MPS112451; lot:CCR6+; stimulation:T007; randomization code:Patient 7 HI.4679.001.NEBNext_Index_3.14
GSM3665242 9: DMSO tissue:PBMC sorted CCR6+ T-cells; barcode:MPS112446; lot:CCR6+; stimulation:DMSO; randomization code:Patient 5 HI.4679.001.NEBNext_Index_4.9
GSM3665243 10: T007 tissue:PBMC sorted CCR6+ T-cells; barcode:MPS112447; lot:CCR6+; stimulation:T007; randomization code:Patient 5 HI.4679.001.NEBNext_Index_5.10
GSM3665244 3: DMSO tissue:PBMC sorted CCR6+ T-cells; barcode:MPS112440; lot:CCR6+; stimulation:DMSO; randomization code:Patient 2 HI.4677.004.NEBNext_Index_6.3
GSM3665245 5: DMSO tissue:PBMC sorted CCR6+ T-cells; barcode:MPS112442; lot:CCR6+; stimulation:DMSO; randomization code:Patient 3 HI.4677.004.NEBNext_Index_7.5
GSM3665246 16: T007 tissue:PBMC sorted CCR6+ T-cells; barcode:MPS112453; lot:CCR6+; stimulation:T007; randomization code:Patient 8 HI.4679.001.NEBNext_Index_8.16
GSM3665247 15: DMSO tissue:PBMC sorted CCR6+ T-cells; barcode:MPS112452; lot:CCR6+; stimulation:DMSO; randomization code:Patient 8 HI.4679.001.NEBNext_Index_9.15
GSM3665232 13: DMSO tissue:PBMC sorted CCR6+ T-cells; barcode:MPS112450; lot:CCR6+; stimulation:DMSO; randomization code:Patient 7 HI.4679.001.NEBNext_Index_1.13
GSM3665233 11: DMSO tissue:PBMC sorted CCR6+ T-cells; barcode:MPS112448; lot:CCR6+; stimulation:DMSO; randomization code:Patient 6 HI.4679.001.NEBNext_Index_12.11
GSM3665234 2: T007 tissue:PBMC sorted CCR6+ T-cells; barcode:MPS112439; lot:CCR6+; stimulation:T007; randomization code:Patient 1 HI.4677.004.NEBNext_Index_13.2
GSM3665235 7: DMSO tissue:PBMC sorted CCR6+ T-cells; barcode:MPS112444; lot:CCR6+; stimulation:DMSO; randomization code:Patient 4 HI.4677.004.NEBNext_Index_14.7
GSM3665236 4: T007 tissue:PBMC sorted CCR6+ T-cells; barcode:MPS112441; lot:CCR6+; stimulation:T007; randomization code:Patient 2 HI.4677.004.NEBNext_Index_15.4
GSM3665237 8: T007 tissue:PBMC sorted CCR6+ T-cells; barcode:MPS112445; lot:CCR6+; stimulation:T007; randomization code:Patient 4 HI.4677.004.NEBNext_Index_16.8
GSM3665238 6: T007 tissue:PBMC sorted CCR6+ T-cells; barcode:MPS112443; lot:CCR6+; stimulation:T007; randomization code:Patient 3 HI.4677.004.NEBNext_Index_18.6
GSM3665239 12: T007 tissue:PBMC sorted CCR6+ T-cells; barcode:MPS112449; lot:CCR6+; stimulation:T007; randomization code:Patient 6 HI.4679.001.NEBNext_Index_19.12
GSM3665240 1: DMSO tissue:PBMC sorted CCR6+ T-cells; barcode:MPS112438; lot:CCR6+; stimulation:DMSO; randomization code:Patient 1 HI.4677.004.NEBNext_Index_2.1
Platform GPL20301  : Illumina HiSeq 4000 (Homo sapiens)
Literature 33089034  
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xCell Plot
* p<0.05 ** p<0.01