Virus Dataset Detail

> Detail information for each sample

Dataset Information
Accession GSE132367
Status 2020/1/13
Title Molecular signatures of dengue virus-specific IL-10/IFN-γ co-producing CD4 T cells and their association with severe dengue disease
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Dengue virus (DENV) infection is associated with a range of clinical manifestations, from self-limiting dengue fever (DF) to life-threatening dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Although DENV-specific CD4 T cells are capable of producing inflammatory cytokines and can acquire cytotoxic functions in previously DENV-exposed individuals, much less is known related to DENV-specific CD4 T cells during acute DENV infection and disease. In this study, we set out to characterize the immune signatures of DENV-specific CD4 T cells during acute infection and investigate whether DENV-specific CD4 T cell response differs between DF and DHF. Previous studies suggest that increased IL-10 level in the blood is associated with severe dengue disease. Here we show evidence of DENV-specific IL-10-producing CD4 T cells during acute DENV infection. More specifically, an IL-10+IFN-γ+ double positive (DP) CD4 T cell population was prominent in acute hospitalized DENV cases but disappeared at the convalescent stage. RNA-sequencing analysis revealed that these cells were associated with gene signatures that marginally overlapped with previously identified signatures of cytotoxic CD4 T cells and type 1 regulatory T (Tr1)-like cells. However, the majority of the genes differentially expressed by DP cells were not related to cytotoxic CD4 T cells or Tr1-like cells. These genes include IL-21, IL-22, CD86, CD109, and CCR1, which are involved in T cell activation, function, cytokine signaling, and migration. Notably, the magnitude of DP cell response was higher in DHF than DF, whereas the gene expression profile of DP cells was similar between DF and DHF. Finally, our data show that DENV-specific DP cells are largely homogeneous based on the expression of 31 selected markers. Overall, this study reveals unique phenotypes of virus-specific IL-10/IFN-γ co-producing CD4 cells and indicates that the quantity but not quality of these cells may be positively correlated with dengue disease severity.
Samples
GSM ID Sample info Characteristics Description
GSM3860975 GS1431_1acute_DF_IL10_pos_100 disease group:DF; donor:GS1431; cell type:PBMC; cell subset:IL10_pos 037_GS1431_1_W_100_merged
GSM3860976 GS1438_1acute_DF_DN_100 disease group:DF; donor:GS1438; cell type:PBMC; cell subset:DN 037_GS1438_1_Y_100_merged
GSM3860977 GS1442_1acute_DF_DN_100 disease group:DF; donor:GS1442; cell type:PBMC; cell subset:DN 037_GS1442_1_Y_100_merged
GSM3860978 GS1431_1acute_DF_DP_100 disease group:DF; donor:GS1431; cell type:PBMC; cell subset:DP 038_GS1431_1_V_100_merged
GSM3860979 GS1442_1acute_DF_IFNg_pos_100 disease group:DF; donor:GS1442; cell type:PBMC; cell subset:IFNg_pos 038_GS1442_1_X_100_merged
GSM3860980 GS1438_1acute_DF_IL10_pos_100 disease group:DF; donor:GS1438; cell type:PBMC; cell subset:IL10_pos 039_GS1438_1_W_100_merged
GSM3860981 GS1442_1acute_DF_IL10_pos_43 disease group:DF; donor:GS1442; cell type:PBMC; cell subset:IL10_pos 039_GS1442_1_W_43_merged
GSM3860982 GS1594_1acute_DHF_DN_100 disease group:DHF; donor:GS1594; cell type:PBMC; cell subset:DN 042_GS1594_1_T_100_merged
GSM3860983 GS1594_1acute_DHF_IFNg_pos_100 disease group:DHF; donor:GS1594; cell type:PBMC; cell subset:IFNg_pos 043_GS1594_1_R_100_merged
GSM3860984 GS1594_1acute_DHF_IL10_pos_100 disease group:DHF; donor:GS1594; cell type:PBMC; cell subset:IL10_pos 044_GS1594_1_Q_100_merged
GSM3860985 GS1594_1acute_DHF_DP_100 disease group:DHF; donor:GS1594; cell type:PBMC; cell subset:DP 045_GS1594_1_P_100_merged
GSM3860986 GS1445_1acute_DHF_DN_100 disease group:DHF; donor:GS1445; cell type:PBMC; cell subset:DN 050_GS1445_1_T_100_merged
GSM3860987 GS1405_1acute_DHF_DN_100 disease group:DHF; donor:GS1405; cell type:PBMC; cell subset:DN 051_GS1405_1_T_100_merged
GSM3860988 GS1445_1acute_DHF_IFNg_pos_100 disease group:DHF; donor:GS1445; cell type:PBMC; cell subset:IFNg_pos 051_GS1445_1_R_100_merged
GSM3860989 GS1405_1acute_DHF_IFNg_pos_47 disease group:DHF; donor:GS1405; cell type:PBMC; cell subset:IFNg_pos 052_GS1405_1_R_47_merged
GSM3860990 GS1445_1acute_DHF_IL10_pos_100 disease group:DHF; donor:GS1445; cell type:PBMC; cell subset:IL10_pos 052_GS1445_1_Q_100_merged
GSM3860991 GS1465_1acute_DHF_DN_100 disease group:DHF; donor:GS1465; cell type:PBMC; cell subset:DN 052_GS1465_1_T_100_merged
GSM3860992 GS1492_1acute_DHF_DN_100 disease group:DHF; donor:GS1492; cell type:PBMC; cell subset:DN 052_GS1492_1_T_100_merged
GSM3860993 GS1445_1acute_DHF_DP_100 disease group:DHF; donor:GS1445; cell type:PBMC; cell subset:DP 053_GS1445_1_P_100_merged
GSM3860994 GS1465_1acute_DHF_IFNg_pos_100 disease group:DHF; donor:GS1465; cell type:PBMC; cell subset:IFNg_pos 053_GS1465_1_R_100_merged
GSM3860995 GS1492_1acute_DHF_IFNg_pos_100 disease group:DHF; donor:GS1492; cell type:PBMC; cell subset:IFNg_pos 053_GS1492_1_R_100_merged
GSM3860996 GS1465_1acute_DHF_IL10_pos_100 disease group:DHF; donor:GS1465; cell type:PBMC; cell subset:IL10_pos 054_GS1465_1_Q_100_merged
GSM3860997 GS1492_1acute_DHF_IL10_pos_75 disease group:DHF; donor:GS1492; cell type:PBMC; cell subset:IL10_pos 054_GS1492_1_Q_75_merged
GSM3860998 GS1465_1acute_DHF_DP_100 disease group:DHF; donor:GS1465; cell type:PBMC; cell subset:DP 055_GS1465_1_P_100_merged
GSM3860999 GS1492_1acute_DHF_DP_100 disease group:DHF; donor:GS1492; cell type:PBMC; cell subset:DP 055_GS1492_1_P_100_merged
GSM3860943 GS1416_1acute_DF_DN_100 disease group:DF; donor:GS1416; cell type:PBMC; cell subset:DN 002_GS1416_1_Y_100_merged
GSM3860944 GS1430_1acute_DF_DN_100 disease group:DF; donor:GS1430; cell type:PBMC; cell subset:DN 002_GS1430_1_Y_100_merged
GSM3860945 GS1435_1acute_DF_DN_100 disease group:DF; donor:GS1435; cell type:PBMC; cell subset:DN 002_GS1435_1_Y_100_merged
GSM3860946 GS1441_1acute_DF_DN_100 disease group:DF; donor:GS1441; cell type:PBMC; cell subset:DN 002_GS1441_1_Y_100_merged
GSM3860947 GS1448_1acute_DF_DN_100 disease group:DF; donor:GS1448; cell type:PBMC; cell subset:DN 002_GS1448_1_Y_100_merged
GSM3860948 GS1430_1acute_DF_IFNg_pos_100 disease group:DF; donor:GS1430; cell type:PBMC; cell subset:IFNg_pos 003_GS1430_1_X_100_merged
GSM3860949 GS1435_1acute_DF_IFNg_pos_100 disease group:DF; donor:GS1435; cell type:PBMC; cell subset:IFNg_pos 003_GS1435_1_X_100_merged
GSM3860950 GS1441_1acute_DF_IFNg_pos_100 disease group:DF; donor:GS1441; cell type:PBMC; cell subset:IFNg_pos 003_GS1441_1_X_100_merged
GSM3860951 GS1448_1acute_DF_IFNg_pos_29 disease group:DF; donor:GS1448; cell type:PBMC; cell subset:IFNg_pos 003_GS1448_1_X_29_merged
GSM3860952 GS1435_1acute_DF_IL10_pos_48 disease group:DF; donor:GS1435; cell type:PBMC; cell subset:IL10_pos 004_GS1435_1_W_48_merged
GSM3860953 GS1430_1acute_DF_DP_100 disease group:DF; donor:GS1430; cell type:PBMC; cell subset:DP 005_GS1430_1_V_100_merged
GSM3860954 GS1499_1acute_DHF_DN_100 disease group:DHF; donor:GS1499; cell type:PBMC; cell subset:DN 010_GS1499_1_T_100_merged
GSM3860955 GS1404_1acute_DHF_DN_100 disease group:DHF; donor:GS1404; cell type:PBMC; cell subset:DN 017_GS1404_1_T_100_merged
GSM3860956 GS1436_1acute_DHF_DN_100 disease group:DHF; donor:GS1436; cell type:PBMC; cell subset:DN 017_GS1436_1_T_100_merged
GSM3860957 GS1458_1acute_DHF_DN_100 disease group:DHF; donor:GS1458; cell type:PBMC; cell subset:DN 017_GS1458_1_T_100_merged
GSM3860958 GS1470_1acute_DHF_DN_100 disease group:DHF; donor:GS1470; cell type:PBMC; cell subset:DN 017_GS1470_1_T_100_merged
GSM3860959 GS1404_1acute_DHF_IFNg_pos_100 disease group:DHF; donor:GS1404; cell type:PBMC; cell subset:IFNg_pos 018_GS1404_1_R_100_merged
GSM3860960 GS1458_1acute_DHF_IFNg_pos_100 disease group:DHF; donor:GS1458; cell type:PBMC; cell subset:IFNg_pos 018_GS1458_1_R_100_merged
GSM3860961 GS1470_1acute_DHF_IFNg_pos_100 disease group:DHF; donor:GS1470; cell type:PBMC; cell subset:IFNg_pos 018_GS1470_1_R_100_merged
GSM3860962 GS1404_1acute_DHF_IL10_pos_100 disease group:DHF; donor:GS1404; cell type:PBMC; cell subset:IL10_pos 019_GS1404_1_Q_100_merged
GSM3860963 GS1458_1acute_DHF_IL10_pos_100 disease group:DHF; donor:GS1458; cell type:PBMC; cell subset:IL10_pos 019_GS1458_1_Q_100_merged
GSM3860964 GS1470_1acute_DHF_IL10_pos_100 disease group:DHF; donor:GS1470; cell type:PBMC; cell subset:IL10_pos 019_GS1470_1_Q_100_merged
GSM3860965 GS1404_1acute_DHF_DP_100 disease group:DHF; donor:GS1404; cell type:PBMC; cell subset:DP 020_GS1404_1_P_100_merged
GSM3860966 GS1458_1acute_DHF_DP_100 disease group:DHF; donor:GS1458; cell type:PBMC; cell subset:DP 020_GS1458_1_P_100_merged
GSM3860967 GS1470_1acute_DHF_DP_100 disease group:DHF; donor:GS1470; cell type:PBMC; cell subset:DP 020_GS1470_1_P_100_merged
GSM3860968 GS1449_1acute_DF_DN_100 disease group:DF; donor:GS1449; cell type:PBMC; cell subset:DN 027_GS1449_1_Y_100_merged
GSM3860969 GS1449_1acute_DF_IFNg_pos_100 disease group:DF; donor:GS1449; cell type:PBMC; cell subset:IFNg_pos 028_GS1449_1_X_100_merged
GSM3860970 GS1449_1acute_DF_IL10_pos_100 disease group:DF; donor:GS1449; cell type:PBMC; cell subset:IL10_pos 029_GS1449_1_W_100_merged
GSM3860971 GS1449_1acute_DF_DP_100 disease group:DF; donor:GS1449; cell type:PBMC; cell subset:DP 030_GS1449_1_V_100_merged
GSM3860972 GS1431_1acute_DF_DN_100 disease group:DF; donor:GS1431; cell type:PBMC; cell subset:DN 035_GS1431_1_Y_100_merged
GSM3860973 GS1431_1acute_DF_IFNg_pos_100 disease group:DF; donor:GS1431; cell type:PBMC; cell subset:IFNg_pos 036_GS1431_1_X_100_merged
GSM3860974 GS1417_1acute_DF_DN_100 disease group:DF; donor:GS1417; cell type:PBMC; cell subset:DN 037_GS1417_1_Y_100_merged
Platform GPL16791  : Illumina HiSeq 2500 (Homo sapiens)
Literature 31875555  
Download Download xCell Data  Download Expression Analysis Data  
xCell Plot
* p<0.05 ** p<0.01