Accession	GSE132777
Status	2020/8/13
Title	ChIP-Seq of IRF4, BATF, and vIRF3 in BC-3 cells
Organism	Homo sapiens
Experiment type	Genome binding/occupancy profiling by high throughput sequencing
Summary	The Kaposi's sarcoma-associated herpesviruses causes several cancers including Kaposi's sarcoma and primary effusion lymphoma (PEL). Our work reveals that the cellular transcription factors interferon regulatory factor 4 (IRF4) and basic leucine zipper ATF-like transcription factor (BATF), as well as the viral interferon regulatory factor 3 (vIRF3) are critical oncogenic dependencies specific to PEL cell lines. To understand the functions of these transcription factors, we performed ChIP-Seq on these proteins in BC-3 PEL cell line. Our results reveal that these oncogenic factors may cooperate to regulate the expression of the same mRNAs.
Samples	GSM ID Sample info Characteristics Description GSM3891772 INPUT_1: Input Rep. 1 cell type:BC-3; chip antibody:none - GSM3891773 IRF4_1: IRF4 Rep. 1 cell type:BC-3; chip antibody:IRF4 (Cell Signaling #15106S) - GSM3891774 IRF4_2: IRF4 Rep. 2 cell type:BC-3; chip antibody:IRF4 (Cell Signaling #15106S) - GSM3891775 IRF4_3: IRF4 Rep. 3 cell type:BC-3; chip antibody:IRF4 (Cell Signaling #15106S) - GSM3891776 VIRF3_1: vIRF3 Rep. 1 cell type:BC-3; chip antibody:vIRF3 (Novus #NB200-167SS) - GSM3891777 VIRF3_2: vIRF3 Rep. 2 cell type:BC-3; chip antibody:vIRF3 (Novus #NB200-167SS) - GSM3891778 VIRF3_3: vIRF3 Rep. 3 cell type:BC-3; chip antibody:vIRF3 (Novus #NB200-167SS) - GSM3891779 BATF_1: BATF Rep. 1 cell type:BC-3; chip antibody:BATF (CDI Laboratories #m14-108) - GSM3891780 BATF_2: BATF Rep. 2 cell type:BC-3; chip antibody:BATF (CDI Laboratories #m14-108) - GSM3891781 BATF_3: BATF Rep. 3 cell type:BC-3; chip antibody:BATF (CDI Laboratories #m14-108) -
Platform	GPL11154  GPL18573  : Illumina HiSeq 2000 (Homo sapiens);Illumina NextSeq 500 (Homo sapiens)
Literature	32843547
Download	Download Genome Binding/Occupancy Profiling Analysis Data