| Accession |
GSE148796
|
| Status |
2020/6/4 |
| Title |
Identification of pathogenic TRAIL-expressing innate immune cells during HIV-1 infection in humanized mice by scRNA-seq |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing |
| Summary |
We employed the single-cell RNA sequencing (scRNA-seq) technology to study HIV-induced transcriptomic change in innate immune cells in lymphoid organs. We performed scRNA-seq on hCD45+hCD3-hCD19- human leukocytes isolated from spleens of humanized NOD/Rag2-/- /γc-/- (NRG) mice transplanted with human CD34+ hematopoietic stem-progenitor cells (NRG-hu HSC mice). We identified major populations of innate immune cells including plasmacytoid dendritic cells (pDC), myeloid dendritic cells (mDC), macrophage, nature killer (NK) cells and innate lymphoid cells (ILC). HIV-1 infection significantly upregulated genes involved in type-I interferon inflammatory pathways in each of the innate immune subsets. Interestingly, we found that the TNF-related apoptosis-inducing ligand (TRAIL) was upregulated in the innate immune populations including pDC, mDC, macrophage, NK and ILC. In summary, we characterized HIV-induced transcriptomic changes of innate immune cells in the spleen at single-cell levels, identified the TRAIL+ innate immune cells and defined an important role of TRAIL signaling pathway in HIV-1 induced CD4+ T cell depletion in vivo. |
| Samples |
| GSM ID |
Sample info |
Characteristics |
Description |
|
GSM4481286
|
A5 HIV-1 |
sample id:A5; condition:HIV-1 infected; humanization procedure:Humanized NRG-hu HSC mice were generated by intrahepatic injection of 2x105 CD34+ hematopoietic progenitor cells derived human fetal liver into newborn (2 to 5 days old) NRG (NOD-Rag2-/- rc-/-) mice.; cell type:hCD45+hCD3-hCD19- splenocyte from humanized mice |
- |
|
GSM4481287
|
A6 HIV-1 |
sample id:A6; condition:HIV-1 infected; humanization procedure:Humanized NRG-hu HSC mice were generated by intrahepatic injection of 2x105 CD34+ hematopoietic progenitor cells derived human fetal liver into newborn (2 to 5 days old) NRG (NOD-Rag2-/- rc-/-) mice.; cell type:hCD45+hCD3-hCD19- splenocyte from humanized mice |
- |
|
GSM4481288
|
A7 Mock |
sample id:A7; condition:Mock; humanization procedure:Humanized NRG-hu HSC mice were generated by intrahepatic injection of 2x105 CD34+ hematopoietic progenitor cells derived human fetal liver into newborn (2 to 5 days old) NRG (NOD-Rag2-/- rc-/-) mice.; cell type:hCD45+hCD3-hCD19- splenocyte from humanized mice |
- |
|
GSM4481289
|
A8 Mock |
sample id:A8; condition:Mock; humanization procedure:Humanized NRG-hu HSC mice were generated by intrahepatic injection of 2x105 CD34+ hematopoietic progenitor cells derived human fetal liver into newborn (2 to 5 days old) NRG (NOD-Rag2-/- rc-/-) mice.; cell type:hCD45+hCD3-hCD19- splenocyte from humanized mice |
- |
|
| Platform |
GPL18573 : Illumina NextSeq 500 (Homo sapiens) |
| Literature |
32406872 |
| Download |
Download scRNA Analysis Data
|
|
scRNA-seq UMAP
|
|
Virus Dataset Detail
