Accession	GSE150390
Status	2020/5/13
Title	Complementation can maintain a quasispecies of drug sensitive and resistant HIV
Organism	Human immunodeficiency virus 1
Experiment type	Genome variation profiling by high throughput sequencing
Summary	The goal of this study was to determine how an HIV quasispecies is maintained in the face of selection. We deep sequenced the HIV provirus from cell populations as well as single cells at different time points from in vitro evolution experiments and found that when a less fit and more fit infect the same cell, they share components (complmentation) and therefore allow the less fit to perpetuate. We reproduced a quasispecies to an HIV reverse transcriptase inhibitor. The drug resistant genotype never completely supplanted the drug sensitive genotype, which stabilized at about 20% of viral sequences. Single-cell sequencing showed that resistant genotype frequency plateaued when cells were co-infected with sensitive and resistant genotypes, suggesting a sharing of viral proteins in co-infected cells (complementation), masking genotypic differences. To test if complementation can confer phenotypic drug resistance, we co-transfected fluorescently labelled molecular clones of sensitive and resistant HIV and observed drug resistance in genotypically sensitive virus from co-transfected cells. Resistant virus preferentially co-infected cells with drug sensitive HIV, explaining initiation of co-infections. Modelling showed that a stable quasispecies could form at the experimental multiplicities of infection. Conclusions: Complementation can lead to a quasispecies in infection environments where multiple infections per cell are common
Samples	GSM ID Sample info Characteristics Description GSM4548117 first run_D10_A1 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548118 first run_D10_A10 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548119 first run_D10_A11 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548120 first run_D10_A12 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548121 first run_D10_A2 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548122 first run_D10_A3 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548123 first run_D10_A4 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548124 first run_D10_A5 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548125 first run_D10_A6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548126 first run_D10_A7 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548127 first run_D10_A8 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548128 first run_D10_A9 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548129 first run_D10_B1 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548130 first run_D10_B10 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548131 first run_D10_B11 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548096 Population_Experiment #3_Day6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 6; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548097 Population_Experiment #3_Day8 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 8; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548098 Population_Experiment #3_Day10 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 10; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548099 Population_Experiment #3_Day12 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 12; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548107 Population_Experiment #2_Day12 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 12; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548108 Population_Experiment #2_Day14 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 14; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548109 Population_Experiment #2_Day16 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 16; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548110 Population_Experiment #2_Day18 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 18; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548111 Population_Experiment #1_Day8 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 8; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548112 Population_Experiment #1_Day10 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 10; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548132 first run_D10_B12 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548113 Population_Experiment #1_Day12 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 12; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548133 first run_D10_B2 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548114 Population_Experiment #1_Day14 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 14; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548134 first run_D10_B3 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548115 Population_Experiment #1_Day16 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 16; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548116 Population_Experiment #1_Day18 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 18; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548135 first run_D10_B4 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548136 first run_D10_B5 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548137 first run_D10_B6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548138 first run_D10_B7 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548139 first run_D10_B8 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548145 first run_D10_C5 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548140 first run_D10_B9 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548146 first run_D10_C6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548141 first run_D10_C1 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548147 first run_D10_C7 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548100 Population_Experiment #3_Day14 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 14; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548148 first run_D10_C8 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548101 Population_Experiment #3_Day16 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 16; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548149 first run_D12_A1 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548102 Population_Experiment #3_Day18 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 18; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548150 first run_D12_A10 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548103 Population_Experiment #2_Day4 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 4; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548151 first run_D12_A11 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548104 Population_Experiment #2_Day6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 6; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548177 first run_D12_C5 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548142 first run_D10_C2 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548105 Population_Experiment #2_Day8 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 8; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548143 first run_D10_C3 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548152 first run_D12_A12 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548106 Population_Experiment #2_Day10 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Day 10; population/single cell:population sequences Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548178 first run_D12_C6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548144 first run_D10_C4 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548153 first run_D12_A2 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548179 first run_D12_C7 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548154 first run_D12_A3 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548200 first run_D18_B5 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548201 first run_D18_B6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548180 first run_D12_C8 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548202 first run_D18_B7 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548155 first run_D12_A4 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548203 first run_D18_B8 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548181 first run_D18_A1 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548204 first run_D18_B9 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548156 first run_D12_A5 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548205 first run_D18_C1 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548182 first run_D18_A10 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548206 first run_D18_C2 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548207 first run_D18_C3 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548208 first run_D18_C4 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548209 first run_D18_C5 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548210 first run_D18_C6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548211 first run_D18_C7 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548183 first run_D18_A11 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548184 first run_D18_A12 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548185 first run_D18_A2 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548186 first run_D18_A3 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548187 first run_D18_A4 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548157 first run_D12_A6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548158 first run_D12_A7 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548159 first run_D12_A8 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548188 first run_D18_A5 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548160 first run_D12_A9 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548189 first run_D18_A6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548212 first run_D18_C8 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548190 first run_D18_A7 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548213 D10_A1 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548168 first run_D12_B5 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548191 first run_D18_A8 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548161 first run_D12_B1 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548225 D10_B1 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548214 D10_A10 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548162 first run_D12_B10 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548226 D10_B10 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548215 D10_A11 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548163 first run_D12_B11 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548169 first run_D12_B6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548192 first run_D18_A9 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548216 D10_A12 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548217 D10_A2 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548227 D10_B11 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548164 first run_D12_B12 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548218 D10_A3 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548170 first run_D12_B7 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548193 first run_D18_B1 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548194 first run_D18_B10 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548195 first run_D18_B11 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548171 first run_D12_B8 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548228 D10_B12 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548219 D10_A4 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548172 first run_D12_B9 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548196 first run_D18_B12 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548165 first run_D12_B2 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548197 first run_D18_B2 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548229 D10_B2 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548220 D10_A5 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548198 first run_D18_B3 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548221 D10_A6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548199 first run_D18_B4 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548173 first run_D12_C1 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548166 first run_D12_B3 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548222 D10_A7 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548252 D18_A7 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548230 D10_B3 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548167 first run_D12_B4 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548223 D10_A8 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548174 first run_D12_C2 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548224 D10_A9 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548271 D18_C5 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548231 D10_B4 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548301 D4_C5 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548253 D18_A8 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548272 D18_C6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548254 D18_A9 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548232 D10_B5 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548255 D18_B1 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548284 D4_A9 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548175 first run_D12_C3 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548256 D18_B10 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548302 D4_C6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548233 D10_B6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548273 D4_A1 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548234 D10_B7 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548274 D4_A10 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548275 D4_A11 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548176 first run_D12_C4 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:First run_Day 12; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548235 D10_B8 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548276 D4_A12 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548277 D4_A2 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548236 D10_B9 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548278 D4_A3 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548279 D4_A4 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548237 D10_C1 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548238 D10_C2 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548257 D18_B11 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548239 D10_C3 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548285 D4_B1 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548258 D18_B12 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548240 D10_C4 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548286 D4_B10 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548287 D4_B11 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548241 D10_C5 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548280 D4_A5 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548259 D18_B2 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548281 D4_A6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548288 D4_B12 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548242 D10_C6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 10; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548260 D18_B3 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548282 D4_A7 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548289 D4_B2 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548243 D18_A1 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548261 D18_B4 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548290 D4_B3 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548248 D18_A3 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548262 D18_B5 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548244 D18_A10 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548245 D18_A11 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548283 D4_A8 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548291 D4_B4 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548263 D18_B6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548292 D4_B5 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548264 D18_B7 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548293 D4_B6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548249 D18_A4 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548265 D18_B8 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548246 D18_A12 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548250 D18_A5 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548294 D4_B7 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548266 D18_B9 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548295 D4_B8 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548267 D18_C1 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548296 D4_B9 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548268 D18_C2 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548297 D4_C1 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548247 D18_A2 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548298 D4_C2 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548269 D18_C3 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548299 D4_C3 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548251 D18_A6 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548270 D18_C4 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 18; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants GSM4548300 D4_C4 cell line:RevCEM E7 cells; treatment:Cells treated every 2 days with Efavirenz; time:Second run_Day 4; population/single cell:single cells Reverse transcriptase region of HIV provirus;~ 300bp amplicon of the Reverse trancriptase region of HIV containing all the Efavirenz resistant mutants
Platform	GPL23868  : Illumina MiSeq (Human immunodeficiency virus 1)
Literature