Virus Dataset Detail

> Detail information for each sample

Dataset Information
Accession GSE153218
Status 2020/12/7
Title Bulk RNA sequencing of SARS-CoV-2 infected alveolar type I- and type II like cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Bulk RNA sequencing was performed on control and SARS-CoV-2 infected 2D bronchioalveolar-like and small airway cultures Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes coronavirus disease 2019 (COVID-19), may result in acute respiratory distress syndrome (ARDS), multi-organ failure and death. The alveolar epithelium is a major target of the virus, but representative models to study virus host interactions in more detail are currently lacking. Here, we describe a human 2D air-liquid interphase culture system which was characterized by confocal-, electron-microscopy and mRNA expression analysis. In this model, alveolar type I- (ATI-L) and type II-like (ATII-L) cells, but also basal cells, neuroendocrine cells and club cells, are grown from 3D self-renewing lung bud tip organoids. These cultures were readily infected by SARS-CoV-2 with both ATI-L and ATII-L cells being targeted. Consequently, significant viral titers were detected and mRNA expression analysis revealed induction of a type I/III interferon response program. Hence, these cultures represent an experimental model for SARS-CoV-2 infection and can be applied for drug screens.
Samples
GSM ID Sample info Characteristics Description
GSM4635984 Bronchioalveo_2_SARS-CoV-2_1 cell type:Bronchioalveolar; treatment:SARS-CoV-2; genotype:Wildtype demultiplexed.22.CS2C8U6;Bulk RNA sequencing of human bronchioalveolar 2D culture infected with SARS-CoV-2 for 72 hours
GSM4635985 Bronchioalveo_2_SARS-CoV-2_2 cell type:Bronchioalveolar; treatment:SARS-CoV-2; genotype:Wildtype demultiplexed.24.CS2C8U6;Bulk RNA sequencing of human bronchioalveolar 2D culture infected with SARS-CoV-2 for 72 hours
GSM4635986 SmallAirway_1_mock cell type:Small airway; treatment:Mock; genotype:Wildtype demultiplexed.34.CS2C8U6;Bulk RNA sequencing of untreated human small airway 2D culture
GSM4635987 SmallAirway_2_mock cell type:Small airway; treatment:Mock; genotype:Wildtype demultiplexed.36.CS2C8U6;Bulk RNA sequencing of untreated human small airway 2D culture
GSM4635988 SmallAirway_1_SARS-CoV-2 cell type:Small airway; treatment:SARS-CoV-2; genotype:Wildtype demultiplexed.38.CS2C8U6;Bulk RNA sequencing of human small airway 2D culture infected with SARS-CoV-2 for 72 hours
GSM4635989 SmallAirway_2_SARS-CoV-2 cell type:Small airway; treatment:SARS-CoV-2; genotype:Wildtype demultiplexed.40.CS2C8U6;Bulk RNA sequencing of human small airway 2D culture infected with SARS-CoV-2 for 72 hours
GSM4635990 fetal3D1 cell type:Lung bud tip; treatment:Mock; genotype:Wildtype demultiplexed.42.CS2C8U6;Bulk RNA sequencing of untreated human fetal lung bud tip organoid culture
GSM4635991 fetal3D2 cell type:Lung bud tip; treatment:Mock; genotype:Wildtype demultiplexed.44.CS2C8U6;Bulk RNA sequencing of untreated human fetal lung bud tip organoid culture
GSM4635992 nonALI1 cell type:Differentiating lung bud tip; treatment:Mock; genotype:Wildtype demultiplexed.46.CS2C8U6;Bulk RNA sequencing of untreated human non-air-liquid interface culture from lung bud tip organoids
GSM4635993 nonALI2 cell type:Differentiating lung bud tip; treatment:Mock; genotype:Wildtype demultiplexed.48.CS2C8U6;Bulk RNA sequencing of untreated human non-air-liquid interface culture from lung bud tip organoids
GSM4635978 Bronchioalveo_1_mock_1 cell type:Bronchioalveolar; treatment:Mock; genotype:Wildtype demultiplexed.2.CS2C8U6;Bulk RNA sequencing of untreated human bronchioalveolar 2D culture
GSM4635979 Bronchioalveo_1_mock_2 cell type:Bronchioalveolar; treatment:Mock; genotype:Wildtype demultiplexed.4.CS2C8U6;Bulk RNA sequencing of untreated human bronchioalveolar 2D culture
GSM4635980 Bronchioalveo_1_SARS-CoV-2_1 cell type:Bronchioalveolar; treatment:SARS-CoV-2; genotype:Wildtype demultiplexed.6.CS2C8U6;Bulk RNA sequencing of human bronchioalveolar 2D culture infected with SARS-CoV-2 for 72 hours
GSM4635981 Bronchioalveo_1_SARS-CoV-2_2 cell type:Bronchioalveolar; treatment:SARS-CoV-2; genotype:Wildtype demultiplexed.8.CS2C8U6;Bulk RNA sequencing of human bronchioalveolar 2D culture infected with SARS-CoV-2 for 72 hours
GSM4635982 Bronchioalveo_2_mock_1 cell type:Bronchioalveolar; treatment:Mock; genotype:Wildtype demultiplexed.18.CS2C8U6;Bulk RNA sequencing of untreated human bronchioalveolar 2D culture
GSM4635983 Bronchioalveo_2_mock_2 cell type:Bronchioalveolar; treatment:Mock; genotype:Wildtype demultiplexed.20.CS2C8U6;Bulk RNA sequencing of untreated human bronchioalveolar 2D culture
Platform GPL18573  : Illumina NextSeq 500 (Homo sapiens)
Literature 33283287  
Download Download xCell Data  Download Expression Analysis Data  
xCell Plot
* p<0.05 ** p<0.01