| Accession |
GSE157829
|
| Status |
2020/9/12 |
| Title |
An Atlas of Immune Cell Exhaustion in HIV-Infected Individuals Revealed by Single-Cell Transcriptomics |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing |
| Summary |
We investigated the effects of HIV infection on immune cell exhaustion at the transcriptomic level by analyzing single-cell RNA sequencing of peripheral blood mononuclear cells from four healthy subjects (37,847 cells) and six HIV-infected donors (28,610 cells). We identified nine immune cell clusters and eight T cell subclusters according to their unique gene expression programs; three of these (exhausted CD4+ and CD8+ T cells and interferon-responsive CD8+ T cells) were detected only in samples from HIV-infected donors. An inhibitory receptor KLRG1 was identified in the exhausted T cell populations and further characterized in HIV infected individuals. We identified a HIV-1 specific exhausted CD8+ T cell population expressing KLRG1, TIGIT, and T-betdimEomeshi markers. Ex-vivo antibody blockade of KLRG1 restored the function of HIV-specific exhausted CD8+ T cells demonstrating the contribution of KLRG1+ population to T cell exhaustion and providing an immunotherapy target to treat HIV chronic infection. Analysis of gene signatures also revealed impairment of B cell and NK cell function in HIV-infected donors. These data provide a comprehensive analysis of gene signatures associated with immune cell exhaustion during HIV infection, which could be useful in understanding exhaustion mechanisms and developing new cure therapies. |
| Samples |
| GSM ID |
Sample info |
Characteristics |
Description |
|
GSM4775588
|
HD1 |
cell type:peripheral blood mononuclear cell; individual:HD1; disease state:control |
- |
|
GSM4775589
|
PID 717 |
cell type:peripheral blood mononuclear cell; individual:PID 717; disease state:HIV |
- |
|
GSM4775590
|
PID 876 |
cell type:peripheral blood mononuclear cell; individual:PID 876; disease state:HIV |
- |
|
GSM4775591
|
PID 529 |
cell type:peripheral blood mononuclear cell; individual:PID 529; disease state:HIV |
- |
|
GSM4775592
|
PID 630 |
cell type:peripheral blood mononuclear cell; individual:PID 630; disease state:HIV |
- |
|
GSM4775593
|
PID 168 |
cell type:peripheral blood mononuclear cell; individual:PID 168; disease state:HIV |
- |
|
GSM4775594
|
PID 471 |
cell type:peripheral blood mononuclear cell; individual:PID 471; disease state:HIV |
- |
|
| Platform |
GPL16791 : Illumina HiSeq 2500 (Homo sapiens) |
| Literature |
32954948 |
| Download |
Download scRNA Analysis Data
|
|
scRNA-seq UMAP
|
|
Virus Dataset Detail
