Virus Dataset Detail

> Detail information for each sample

Dataset Information
Accession GSE163707
Status 2021/3/1
Title Human plasmacytoid dendritic cells mount a distinct antiviral response to virus-infected cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Plasmacytoid dendritic cells (pDCs) can rapidly produce interferons and other soluble factors in response to extracellular viruses or virus mimics such as CpG-containing DNA. pDCs can also recognize live cells infected with certain RNA viruses, but the relevance and functional consequences of such recognition remain unclear. We studied the response of primary DCs to the prototypical persistent DNA virus, the human cytomegalovirus (CMV). Human pDCs responded poorly to free CMV but strongly to live CMV-infected fibroblasts, in a process that involved integrin-mediated adhesion, transfer of viral DNA to pDCs and its recognition through TLR9. Compared to transient polyfunctional responses to CpG or free influenza virus, pDC response to CMV-infected cells was long-lasting, dominated by the production of type I (IFN-I) and type III (IFN-III) interferons, and lacked diversification into functionally distinct populations. Similarly, pDC activation by influenza-infected lung epithelial cells was highly efficient, prolonged and dominated by interferon production. Prolonged pDC activation by CMV-infected cells facilitated the activation of natural killer cells that are critical for CMV control. Finally, patients with CMV viremia harbored phenotypically activated pDCs and increased levels of IFN-I and IFN-III in circulation. Thus, recognition of live infected cells is a common mechanism of virus detection by pDCs that elicits a unique antiviral response program.
Samples
GSM ID Sample info Characteristics Description
GSM4984122 BR.a4 donor number:Donor 1; cell type:pDCs; treatment:uninfected MRC5 cells; time:2 hr -
GSM4984123 BR.a5 donor number:Donor 2; cell type:pDCs; treatment:uninfected MRC5 cells; time:2 hr -
GSM4984124 BR.a6 donor number:Donor 3; cell type:pDCs; treatment:uninfected MRC5 cells; time:2 hr -
GSM4984125 BR.a7 donor number:Donor 1; cell type:pDCs; treatment:CMV-infected MRC5 cells; time:2 hr -
GSM4984126 BR.a8 donor number:Donor 2; cell type:pDCs; treatment:CMV-infected MRC5 cells; time:2 hr -
GSM4984127 BR.a9 donor number:Donor 3; cell type:pDCs; treatment:CMV-infected MRC5 cells; time:2 hr -
GSM4984128 BR.a10 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984129 BR.a11 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984130 BR.a12 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984131 BR.b1 donor number:Donor 1; cell type:pDCs; treatment:CpG (2.5 uM); time:4 hr -
GSM4984132 BR.b2 donor number:Donor 2; cell type:pDCs; treatment:CpG (2.5 uM); time:4 hr -
GSM4984133 BR.b3 donor number:Donor 3; cell type:pDCs; treatment:CpG (2.5 uM); time:4 hr -
GSM4984134 BR.b4 donor number:Donor 1; cell type:pDCs; treatment:uninfected MRC5 cells; time:4 hr -
GSM4984135 BR.b5 donor number:Donor 2; cell type:pDCs; treatment:uninfected MRC5 cells; time:4 hr -
GSM4984136 BR.b6 donor number:Donor 3; cell type:pDCs; treatment:uninfected MRC5 cells; time:4 hr -
GSM4984137 BR.b7 donor number:Donor 1; cell type:pDCs; treatment:CMV-infected MRC5 cells; time:4 hr -
GSM4984138 BR.b8 donor number:Donor 2; cell type:pDCs; treatment:CMV-infected MRC5 cells; time:4 hr -
GSM4984139 BR.b9 donor number:Donor 3; cell type:pDCs; treatment:CMV-infected MRC5 cells; time:4 hr -
GSM4984140 BR.b10 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984141 BR.b11 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984142 BR.b12 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984143 BR.c1 donor number:Donor 1; cell type:pDCs; treatment:none; time:4 hr -
GSM4984144 BR.c2 donor number:Donor 2; cell type:pDCs; treatment:none; time:4 hr -
GSM4984145 BR.c3 donor number:Donor 3; cell type:pDCs; treatment:none; time:4 hr -
GSM4984146 BR.c4 donor number:Donor 1; cell type:pDCs; treatment:free CMV (10 MOI); time:4 hr -
GSM4984208 BR.h6 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984209 BR.h7 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984210 BR.h8 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984147 BR.c5 donor number:Donor 2; cell type:pDCs; treatment:free CMV (10 MOI); time:4 hr -
GSM4984211 BR.h9 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984212 BR.h10 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984213 BR.h11 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984148 BR.c6 donor number:Donor 3; cell type:pDCs; treatment:free CMV (10 MOI); time:4 hr -
GSM4984149 BR.c7 donor number:Donor 1; cell type:pDCs; treatment:IL3; time:4 hr -
GSM4984214 BR.h12 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984150 BR.c8 donor number:Donor 2; cell type:pDCs; treatment:IL3; time:4 hr -
GSM4984151 BR.c9 donor number:Donor 3; cell type:pDCs; treatment:IL3; time:4 hr -
GSM4984152 BR.c10 donor number:Donor 1; cell type:pDCs; treatment:IL3 + CMV (10 MOI); time:4 hr -
GSM4984153 BR.c11 donor number:Donor 2; cell type:pDCs; treatment:IL3 + CMV (10 MOI); time:4 hr -
GSM4984154 BR.c12 donor number:Donor 3; cell type:pDCs; treatment:IL3 + CMV (10 MOI); time:4 hr -
GSM4984155 BR.d1 donor number:Donor 1; cell type:pDCs; treatment:CpG (2.5 uM); time:8 hr -
GSM4984156 BR.d2 donor number:Donor 2; cell type:pDCs; treatment:CpG (2.5 uM); time:8 hr -
GSM4984157 BR.d3 donor number:Donor 3; cell type:pDCs; treatment:CpG (2.5 uM); time:8 hr -
GSM4984119 BR.a1 donor number:Donor 1; cell type:pDCs; treatment:CpG (2.5 uM); time:2 hr -
GSM4984158 BR.d4 donor number:Donor 1; cell type:pDCs; treatment:uninfected MRC5 cells; time:8 hr -
GSM4984120 BR.a2 donor number:Donor 2; cell type:pDCs; treatment:CpG (2.5 uM); time:2 hr -
GSM4984159 BR.d5 donor number:Donor 2; cell type:pDCs; treatment:uninfected MRC5 cells; time:8 hr -
GSM4984121 BR.a3 donor number:Donor 3; cell type:pDCs; treatment:CpG (2.5 uM); time:2 hr -
GSM4984160 BR.d6 donor number:Donor 3; cell type:pDCs; treatment:uninfected MRC5 cells; time:8 hr -
GSM4984161 BR.d7 donor number:Donor 1; cell type:pDCs; treatment:CMV-infected MRC5 cells; time:8 hr -
GSM4984162 BR.d8 donor number:Donor 2; cell type:pDCs; treatment:CMV-infected MRC5 cells; time:8 hr -
GSM4984163 BR.d9 donor number:Donor 3; cell type:pDCs; treatment:CMV-infected MRC5 cells; time:8 hr -
GSM4984164 BR.d10 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984165 BR.d11 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984166 BR.d12 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984167 BR.e1 donor number:Donor 1; cell type:pDCs; treatment:none; time:8 hr -
GSM4984168 BR.e2 donor number:Donor 2; cell type:pDCs; treatment:none; time:8 hr -
GSM4984169 BR.e3 donor number:Donor 3; cell type:pDCs; treatment:none; time:8 hr -
GSM4984170 BR.e4 donor number:Donor 1; cell type:pDCs; treatment:free CMV (10 MOI); time:8 hr -
GSM4984171 BR.e5 donor number:Donor 2; cell type:pDCs; treatment:free CMV (10 MOI); time:8 hr -
GSM4984172 BR.e6 donor number:Donor 3; cell type:pDCs; treatment:free CMV (10 MOI); time:8 hr -
GSM4984173 BR.e7 donor number:Donor 1; cell type:pDCs; treatment:IL3; time:8 hr -
GSM4984174 BR.e8 donor number:Donor 2; cell type:pDCs; treatment:IL3; time:8 hr -
GSM4984175 BR.e9 donor number:Donor 3; cell type:pDCs; treatment:IL3; time:8 hr -
GSM4984176 BR.e10 donor number:Donor 1; cell type:pDCs; treatment:IL3 + CMV (10 MOI); time:8 hr -
GSM4984177 BR.e11 donor number:Donor 2; cell type:pDCs; treatment:IL3 + CMV (10 MOI); time:8 hr -
GSM4984178 BR.e12 donor number:Donor 3; cell type:pDCs; treatment:IL3 + CMV (10 MOI); time:8 hr -
GSM4984179 BR.f1 donor number:Donor 1; cell type:pDCs; treatment:CpG (2.5 uM); time:16 hr -
GSM4984180 BR.f2 donor number:Donor 2; cell type:pDCs; treatment:CpG (2.5 uM); time:16 hr -
GSM4984181 BR.f3 donor number:Donor 3; cell type:pDCs; treatment:CpG (2.5 uM); time:16 hr -
GSM4984182 BR.f4 donor number:Donor 1; cell type:pDCs; treatment:uninfected MRC5 cells; time:16 hr -
GSM4984183 BR.f5 donor number:Donor 2; cell type:pDCs; treatment:uninfected MRC5 cells; time:16 hr -
GSM4984184 BR.f6 donor number:Donor 3; cell type:pDCs; treatment:uninfected MRC5 cells; time:16 hr -
GSM4984185 BR.f7 donor number:Donor 1; cell type:pDCs; treatment:CMV-infected MRC5 cells; time:16 hr -
GSM4984186 BR.f8 donor number:Donor 2; cell type:pDCs; treatment:CMV-infected MRC5 cells; time:16 hr -
GSM4984187 BR.f9 donor number:Donor 3; cell type:pDCs; treatment:CMV-infected MRC5 cells; time:16 hr -
GSM4984188 BR.f10 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984189 BR.f11 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984190 BR.f12 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984191 BR.g1 donor number:Donor 1; cell type:pDCs; treatment:CpG (2.5 uM); time:32 hr -
GSM4984192 BR.g2 donor number:Donor 2; cell type:pDCs; treatment:CpG (2.5 uM); time:32 hr -
GSM4984193 BR.g3 donor number:Donor 3; cell type:pDCs; treatment:CpG (2.5 uM); time:32 hr -
GSM4984194 BR.g4 donor number:Donor 1; cell type:pDCs; treatment:uninfected MRC5 cells; time:32 hr -
GSM4984195 BR.g5 donor number:Donor 2; cell type:pDCs; treatment:uninfected MRC5 cells; time:32 hr -
GSM4984196 BR.g6 donor number:Donor 3; cell type:pDCs; treatment:uninfected MRC5 cells; time:32 hr -
GSM4984197 BR.g7 donor number:Donor 1; cell type:pDCs; treatment:CMV-infected MRC5 cells; time:32 hr -
GSM4984198 BR.g8 donor number:Donor 2; cell type:pDCs; treatment:CMV-infected MRC5 cells; time:32 hr -
GSM4984199 BR.g9 donor number:Donor 3; cell type:pDCs; treatment:CMV-infected MRC5 cells; time:32 hr -
GSM4984200 BR.g10 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984201 BR.g11 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984202 BR.g12 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984203 BR.h1 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984204 BR.h2 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984205 BR.h3 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984206 BR.h4 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
GSM4984207 BR.h5 donor number:n/a; cell type:n/a; treatment:blank; time:blank -
Platform GPL18573  GPL29107  : Illumina NextSeq 500 (Homo sapiens);Illumina NextSeq 500 (blank sample)
Literature 33811059  
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xCell Plot
* p<0.05 ** p<0.01