Virus Dataset Detail

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Dataset Information
Accession GSE164264
Status 2021/12/31
Title RNA sequencing of Chlamydia trachomatis-stimulated HIV-1-infected CD4 T cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Sexually transmitted infections (STIs) are commonly reported among HIV-1 infected patients. The increasing prevalence of the most common STI, Chlamydia trachomatis (CT), among HIV-1 infected people suggests a role in HIV-1 infectivity. However, the mechanisms modulating the enhancement of HIV-1 infectivity during HIV-1/STIs coinfection remain elusive. The stimulation of CD4 T cells during CT infection may modulate the expression of specific genes, which in turn enhance the susceptibility and infectivity of CT-specific CD4 T cells to HIV-1 infection. After three days of CT stimulation of PBMCs followed by 3 days of HIV-1 infection, we observed a significant increase in HIV-1 p24 levels among clinically diagnosed C. trachomatis-infected patients as compared to cells from healthy donors. Similarly, ex vivo CT antigen-stimulated PBMCs from healthy donors showed enhanced susceptibility to HIV-1 as compared to unstimulated PBMCs. CT-specific CD4 T cells also harbour more HIV-1 copy numbers as compared to healthy unstimulated CD4 T cells. RNA-seq data revealed the upregulation of CCR chemokine receptors and cytokines in CD4 T cells from CT-stimulated CD4 T cells infected with HIV-1.
Samples
GSM ID Sample info Characteristics Description
GSM5005389 NC_CD4_1 tissue source:blood; cell type:PBMCs; treatment:none; untreated; cell subtype:CD4+ T cells processed data file:;NC_CD4.xls
GSM5005390 NC_CD4_2 tissue source:blood; cell type:PBMCs; treatment:none; untreated; cell subtype:CD4+ T cells processed data file:;NC_CD4.xls
GSM5005391 HIV_CD4_1 tissue source:blood; cell type:PBMCs; treatment:HIV-1 infection; cell subtype:CD4+ T cells processed data file:;HIV_CD4.xls.xlsx
GSM5005392 HIV_CD4_2 tissue source:blood; cell type:PBMCs; treatment:HIV-1 infection; cell subtype:CD4+ T cells processed data file:;HIV_CD4.xls.xlsx
GSM5005393 CT_HIV_CD4_1 tissue source:blood; cell type:PBMCs; treatment:C. trachomatis stimulation and HIV-1 coinfection; cell subtype:CD4+ T cells processed data file:;CT_HIV_CD4.xls.xlsx
GSM5005394 CT_HIV_CD4_2 tissue source:blood; cell type:PBMCs; treatment:C. trachomatis stimulation and HIV-1 coinfection; cell subtype:CD4+ T cells processed data file:;CT_HIV_CD4.xls.xlsx
Platform GPL23227  : BGISEQ-500 (Homo sapiens)
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xCell Plot
* p<0.05 ** p<0.01