Accession	GSE164842
Status	2021/3/5
Title	Pol2 and H3K27Ac and H3K27me3 occupancy of the viral and host genomes in HCMV infected Kasumi-3 cells at 24 hpi
Organism	Homo sapiens;Human betaherpesvirus 5
Experiment type	Genome binding/occupancy profiling by high throughput sequencing
Summary	Purpose: to compare H3K27Ac and H3K27me3 in HCMV infected and mock infected Kasumi-3 cells at 24 hours post infection and analyze H3K27Ac and H3K27me3 occupancy of the viral genome in GFP+ infected cells
Samples	GSM ID Sample info Characteristics Description GSM5020855 input_mock_infected_cells_Rep1 treatment: live mock-infected, 24hpi;chip antibody: none;cell line: Kasumi-3 - GSM5020856 input_mock_infected_cells_Rep2 treatment: live mock-infected, 24hpi;chip antibody: none;cell line: Kasumi-3 - GSM5020857 input_mock_infected_cells_Rep3 treatment: live mock-infected, 24hpi;chip antibody: none;cell line: Kasumi-3 - GSM5020858 H3K27Ac_mock-infected_cells_Rep1 treatment: live mock-infected, 24hpi;chip antibody: Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies);cell line: Kasumi-3 - GSM5020859 H3K27Ac_mock-infected_cells_Rep2 treatment: live mock-infected, 24hpi;chip antibody: Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies);cell line: Kasumi-3 - GSM5020860 H3K27Ac_mock-infected_cells_Rep3 treatment: live mock-infected, 24hpi;chip antibody: Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies);cell line: Kasumi-3 - GSM5020861 H3K27me3_mock_infected_cells_Rep1 treatment: live mock-infected, 24hpi;chip antibody: Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies);cell line: Kasumi-3 - GSM5020862 H3K27me3_mock_infected_cells_Rep2 treatment: live mock-infected, 24hpi;chip antibody: Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies);cell line: Kasumi-3 - GSM5020863 H3K27me3_mock_infected_cells_Rep3 treatment: live mock-infected, 24hpi;chip antibody: Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies);cell line: Kasumi-3 - GSM5020864 input_24hpi_Rep1 treatment: live GFP+ infected, 24hpi;chip antibody: none;cell line: Kasumi-3 - GSM5020865 input_24hpi_Rep2 treatment: live GFP+ infected, 24hpi;chip antibody: none;cell line: Kasumi-3 - GSM5020866 input_24hpi_Rep3 treatment: live GFP+ infected, 24hpi;chip antibody: none;cell line: Kasumi-3 - GSM5020867 H3K27Ac_24hpi_Rep1 treatment: live GFP+ infected, 24hpi;chip antibody: Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies);cell line: Kasumi-3 - GSM5020868 H3K27Ac_24hpi_Rep2 treatment: live GFP+ infected, 24hpi;chip antibody: Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies);cell line: Kasumi-3 - GSM5020869 H3K27Ac_24hpi_Rep3 treatment: live GFP+ infected, 24hpi;chip antibody: Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies);cell line: Kasumi-3 - GSM5020870 H3K27me3_24hpi_Rep1 treatment: live GFP+ infected, 24hpi;chip antibody: Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies);cell line: Kasumi-3 - GSM5020871 H3K27me3_24hpi_Rep2 treatment: live GFP+ infected, 24hpi;chip antibody: Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies);cell line: Kasumi-3 - GSM5020872 H3K27me3_24hpi_Rep3 treatment: live GFP+ infected, 24hpi;chip antibody: Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies);cell line: Kasumi-3 - GSM5020873 H3_24hpi_Rep1 treatment: live GFP+ infected, 24hpi;chip antibody: Histone H3 Antibody (ChIP Formulated) #2650 (Cell Signaling Technologies);cell line: Kasumi-3 - GSM5020874 H3_24hpi_Rep2 treatment: live GFP+ infected, 24hpi;chip antibody: Histone H3 Antibody (ChIP Formulated) #2650 (Cell Signaling Technologies);cell line: Kasumi-3 - GSM5020875 H3_24hpi_Rep3 treatment: live GFP+ infected, 24hpi;chip antibody: Histone H3 Antibody (ChIP Formulated) #2650 (Cell Signaling Technologies);cell line: Kasumi-3 -
Platform	GPL20301  GPL29603  : Illumina HiSeq 4000 (Homo sapiens);GPL29603 Illumina HiSeq 4000 (Homo sapiens; Human betaherpesvirus 5)
Literature	33731453