Accession	GSE164844
Status	2021/3/5
Title	Pol2 and H3K27Ac and H3K27me3 occupancy of the viral genome in HCMV infected Kasumi-3 cells at early times post infection
Organism	Human betaherpesvirus 5
Experiment type	Genome binding/occupancy profiling by high throughput sequencing
Summary	Purpose: to compare Pol2 and H3K27Ac and H3K27me3 in HCMV infected Kasumi-3 cells at 4 and 24 hours post infection
Samples	GSM ID Sample info Characteristics Description GSM5020886 input_4hpi_Rep1 treatment: HCMV infected, 4 hr;chip antibody: none;cell line: Kasumi-3 ChIPseq GSM5020887 input_4hpi_Rep2 treatment: HCMV infected, 4 hr;chip antibody: none;cell line: Kasumi-3 ChIPseq GSM5020888 input_4hpi_Rep3 treatment: HCMV infected, 4 hr;chip antibody: none;cell line: Kasumi-3 ChIPseq GSM5020889 Pol2_4hpi_Rep1 treatment: HCMV infected, 24 hr;chip antibody: Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies);cell line: Kasumi-3 ChIPseq GSM5020890 Pol2_4hpi_Rep2 treatment: HCMV infected, 24 hr;chip antibody: Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies);cell line: Kasumi-3 ChIPseq GSM5020891 Pol2_4hpi_Rep3 treatment: HCMV infected, 24 hr;chip antibody: Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies);cell line: Kasumi-3 ChIPseq GSM5020892 H3K27Ac_4hpi_Rep1 treatment: HCMV infected, 4 hr;chip antibody: Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies);cell line: Kasumi-3 ChIPseq GSM5020893 H3K27Ac_4hpi_Rep2 treatment: HCMV infected, 4 hr;chip antibody: Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies);cell line: Kasumi-3 ChIPseq GSM5020894 H3K27Ac_4hpi_Rep3 treatment: HCMV infected, 4 hr;chip antibody: Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies);cell line: Kasumi-3 ChIPseq GSM5020895 H3K27me3_4hpi_Rep1 treatment: HCMV infected, 4 hr;chip antibody: Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies);cell line: Kasumi-3 ChIPseq GSM5020896 H3K27me3_4hpi_Rep2 treatment: HCMV infected, 4 hr;chip antibody: Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies);cell line: Kasumi-3 ChIPseq GSM5020897 H3K27me3_4hpi_Rep3 treatment: HCMV infected, 4 hr;chip antibody: Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies);cell line: Kasumi-3 ChIPseq GSM5020898 input_24hpi_Rep1 treatment: HCMV infected, 24 hr;chip antibody: none;cell line: Kasumi-3 ChIPseq GSM5020899 input_24hpi_Rep2 treatment: HCMV infected, 24 hr;chip antibody: none;cell line: Kasumi-3 ChIPseq GSM5020900 input_24hpi_Rep3 treatment: HCMV infected, 24 hr;chip antibody: none;cell line: Kasumi-3 ChIPseq GSM5020901 Pol2_24hpi_Rep1 treatment: HCMV infected, 24 hr;chip antibody: Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies);cell line: Kasumi-3 ChIPseq GSM5020902 Pol2_24hpi_Rep2 treatment: HCMV infected, 24 hr;chip antibody: Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies);cell line: Kasumi-3 ChIPseq GSM5020903 Pol2_24hpi_Rep3 treatment: HCMV infected, 24 hr;chip antibody: Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies);cell line: Kasumi-3 ChIPseq GSM5020904 H3K27Ac_24hpi_Rep1 treatment: HCMV infected, 24 hr;chip antibody: Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies);cell line: Kasumi-3 ChIPseq GSM5020905 H3K27Ac_24hpi_Rep2 treatment: HCMV infected, 24 hr;chip antibody: Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies);cell line: Kasumi-3 ChIPseq GSM5020906 H3K27Ac_24hpi_Rep3 treatment: HCMV infected, 24 hr;chip antibody: Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies);cell line: Kasumi-3 ChIPseq GSM5020907 H3K27me3_24hpi_Rep1 treatment: HCMV infected, 24 hr;chip antibody: Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies);cell line: Kasumi-3 ChIPseq GSM5020908 H3K27me3_24hpi_Rep2 treatment: HCMV infected, 24 hr;chip antibody: Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies);cell line: Kasumi-3 ChIPseq GSM5020909 H3K27me3_24hpi_Rep3 treatment: HCMV infected, 24 hr;chip antibody: Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies);cell line: Kasumi-3 ChIPseq
Platform	GPL29601  GPL29602  : Illumina HiSeq 4000 (Human betaherpesvirus 5);Illumina NovaSeq 6000 (Human betaherpesvirus 5)
Literature	33731453