Accession	GSE164861
Status	2021/3/5
Title	Epigenetic reprogramming of host and viral genes by Human Cytomegalovirus infection in Kasumi-3 myeloid progenitor cells at early times post-infection
Organism	Homo sapiens;Human betaherpesvirus 5
Experiment type	Expression profiling by high throughput sequencing;Genome binding/occupancy profiling by high throughput sequencing
Summary	This SuperSeries is composed of the following SubSeries: GSE164839 Transcriptome analysis of HCMV infected and mock-infected Kasumi-3 cells at 24 hours post infection GSE164840 Viral transcriptome analysis of HCMV infected Kasumi-3 cells at early time post infection GSE164841 Chromatin accessibilty analysis of HCMV infected and mock-infected Kasumi-3 cells at 24 hours post infection GSE164842 Pol2 and H3K27Ac and H3K27me3 occupancy of the viral and host genomes in HCMV infected Kasumi-3 cells at 24 hpi GSE164843 Comparison of HCMV nascent and steady state transcriptomes expresssed in the Kasumi-3 latency model at early times post-infection GSE164844 Pol2 and H3K27Ac and H3K27me3 occupancy of the viral genome in HCMV infected Kasumi-3 cells at early times post infection GSE164845 Pol2 occupancy of the viral and host genomes in HCMV infected Kasumi-3 cells at 24 hpi
Samples	GSM ID Sample info Characteristics Description GSM5020846 24hr_K3_Unsorted_Rep1 treatment:HCMV infected, 24 hr; rna type:total RNA; cell line:Kasumi-3 RNA-seq GSM5020897 H3K27me3_4hpi_Rep3 treatment:HCMV infected, 4 hr; chip antibody:Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies); cell line:Kasumi-3 ChIPseq GSM5020847 24hr_K3_Unsorted_Rep2 treatment:HCMV infected, 24 hr; rna type:total RNA; cell line:Kasumi-3 RNA-seq GSM5020848 24hr_K3_Unsorted_Rep3 treatment:HCMV infected, 24 hr; rna type:total RNA; cell line:Kasumi-3 RNA-seq GSM5020898 input_24hpi_Rep1 treatment:HCMV infected, 24 hr; chip antibody:none; cell line:Kasumi-3 ChIPseq GSM5020849 24hr_K3_Uninfected_Rep1 treatment:mock-infected, 24 hr; cell line:Kasumi-3; barcode:Ad2.1: TAAGGCGA ATACseq GSM5020850 24hr_K3_Uninfected_Rep2 treatment:mock-infected, 24 hr; cell line:Kasumi-3; barcode:Ad2.10: CGAGGCTG ATACseq GSM5020899 input_24hpi_Rep2 treatment:HCMV infected, 24 hr; chip antibody:none; cell line:Kasumi-3 ChIPseq GSM5020851 24hr_K3_Uninfected_Rep3 treatment:mock-infected, 24 hr; cell line:Kasumi-3; barcode:Ad.6: TAGGCATG ATACseq GSM5020900 input_24hpi_Rep3 treatment:HCMV infected, 24 hr; chip antibody:none; cell line:Kasumi-3 ChIPseq GSM5020901 Pol2_24hpi_Rep1 treatment:HCMV infected, 24 hr; chip antibody:Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies); cell line:Kasumi-3 ChIPseq GSM5020902 Pol2_24hpi_Rep2 treatment:HCMV infected, 24 hr; chip antibody:Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies); cell line:Kasumi-3 ChIPseq GSM5020903 Pol2_24hpi_Rep3 treatment:HCMV infected, 24 hr; chip antibody:Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies); cell line:Kasumi-3 ChIPseq GSM5020904 H3K27Ac_24hpi_Rep1 treatment:HCMV infected, 24 hr; chip antibody:Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies); cell line:Kasumi-3 ChIPseq GSM5020905 H3K27Ac_24hpi_Rep2 treatment:HCMV infected, 24 hr; chip antibody:Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies); cell line:Kasumi-3 ChIPseq GSM5020906 H3K27Ac_24hpi_Rep3 treatment:HCMV infected, 24 hr; chip antibody:Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies); cell line:Kasumi-3 ChIPseq GSM5020907 H3K27me3_24hpi_Rep1 treatment:HCMV infected, 24 hr; chip antibody:Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies); cell line:Kasumi-3 ChIPseq GSM5020908 H3K27me3_24hpi_Rep2 treatment:HCMV infected, 24 hr; chip antibody:Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies); cell line:Kasumi-3 ChIPseq GSM5020909 H3K27me3_24hpi_Rep3 treatment:HCMV infected, 24 hr; chip antibody:Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies); cell line:Kasumi-3 ChIPseq GSM5020852 24hr_K3_Infected_Rep1 treatment:HCMV infected, 24 hr; cell line:Kasumi-3; barcode:Ad2.2: CGTACTAG ATACseq GSM5020853 24hr_K3_Infected_Rep2 treatment:HCMV infected, 24 hr; cell line:Kasumi-3; barcode:Ad2.11: AAGAGGCA ATACseq GSM5020854 24hr_K3_Infected_Rep3 treatment:HCMV infected, 24 hr; cell line:Kasumi-3; barcode:Ad2.7:CTCTCTAC ATACseq GSM5020855 input_mock_infected_cells_Rep1 treatment:live mock-infected, 24hpi; chip antibody:none; cell line:Kasumi-3 - GSM5020856 input_mock_infected_cells_Rep2 treatment:live mock-infected, 24hpi; chip antibody:none; cell line:Kasumi-3 - GSM5020857 input_mock_infected_cells_Rep3 treatment:live mock-infected, 24hpi; chip antibody:none; cell line:Kasumi-3 - GSM5020858 H3K27Ac_mock-infected_cells_Rep1 treatment:live mock-infected, 24hpi; chip antibody:Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies); cell line:Kasumi-3 - GSM5020859 H3K27Ac_mock-infected_cells_Rep2 treatment:live mock-infected, 24hpi; chip antibody:Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies); cell line:Kasumi-3 - GSM5020860 H3K27Ac_mock-infected_cells_Rep3 treatment:live mock-infected, 24hpi; chip antibody:Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies); cell line:Kasumi-3 - GSM5020861 H3K27me3_mock_infected_cells_Rep1 treatment:live mock-infected, 24hpi; chip antibody:Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies); cell line:Kasumi-3 - GSM5020862 H3K27me3_mock_infected_cells_Rep2 treatment:live mock-infected, 24hpi; chip antibody:Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies); cell line:Kasumi-3 - GSM5020863 H3K27me3_mock_infected_cells_Rep3 treatment:live mock-infected, 24hpi; chip antibody:Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies); cell line:Kasumi-3 - GSM5020864 input_24hpi_Rep1 treatment:live GFP+ infected, 24hpi; chip antibody:none; cell line:Kasumi-3 - GSM5020865 input_24hpi_Rep2 treatment:live GFP+ infected, 24hpi; chip antibody:none; cell line:Kasumi-3 - GSM5020866 input_24hpi_Rep3 treatment:live GFP+ infected, 24hpi; chip antibody:none; cell line:Kasumi-3 - GSM5020867 H3K27Ac_24hpi_Rep1 treatment:live GFP+ infected, 24hpi; chip antibody:Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies); cell line:Kasumi-3 - GSM5020868 H3K27Ac_24hpi_Rep2 treatment:live GFP+ infected, 24hpi; chip antibody:Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies); cell line:Kasumi-3 - GSM5020869 H3K27Ac_24hpi_Rep3 treatment:live GFP+ infected, 24hpi; chip antibody:Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies); cell line:Kasumi-3 - GSM5020870 H3K27me3_24hpi_Rep1 treatment:live GFP+ infected, 24hpi; chip antibody:Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies); cell line:Kasumi-3 - GSM5020871 H3K27me3_24hpi_Rep2 treatment:live GFP+ infected, 24hpi; chip antibody:Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies); cell line:Kasumi-3 - GSM5020872 H3K27me3_24hpi_Rep3 treatment:live GFP+ infected, 24hpi; chip antibody:Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies); cell line:Kasumi-3 - GSM5020873 H3_24hpi_Rep1 treatment:live GFP+ infected, 24hpi; chip antibody:Histone H3 Antibody (ChIP Formulated) #2650 (Cell Signaling Technologies); cell line:Kasumi-3 - GSM5020874 H3_24hpi_Rep2 treatment:live GFP+ infected, 24hpi; chip antibody:Histone H3 Antibody (ChIP Formulated) #2650 (Cell Signaling Technologies); cell line:Kasumi-3 - GSM5020875 H3_24hpi_Rep3 treatment:live GFP+ infected, 24hpi; chip antibody:Histone H3 Antibody (ChIP Formulated) #2650 (Cell Signaling Technologies); cell line:Kasumi-3 - GSM5020876 Nascent_RNA_Infected_K3_Rep1 treatment:HCMV infected, 24 hr, EU-labeled, biotinylated RNA purified; rna type:nascent RNA; cell line:Kasumi-3 RNA-seq GSM5020877 Nascent_RNA_Infected_K3_Rep2 treatment:HCMV infected, 24 hr, EU-labeled, biotinylated RNA purified; rna type:nascent RNA; cell line:Kasumi-3 RNA-seq GSM5020878 Nascent_RNA_Infected_K3_Rep3 treatment:HCMV infected, 24 hr, EU-labeled, biotinylated RNA purified; rna type:nascent RNA; cell line:Kasumi-3 RNA-seq GSM5020879 Nascent_RNA_Uninfected_K3_Rep1 treatment:mock infected, 24 hr, EU-labeled, biotinylated RNA purified; rna type:nascent RNA; cell line:Kasumi-3 RNA-seq GSM5020880 Nascent_RNA_Uninfected_K3_Rep2 treatment:mock infected, 24 hr, EU-labeled, biotinylated RNA purified; rna type:nascent RNA; cell line:Kasumi-3 RNA-seq GSM5020881 Total_RNA_Infected_K3_Rep1 treatment:HCMV infected, 24 hr, EU-labeled total RNA; rna type:steady state RNA; cell line:Kasumi-3 RNA-seq GSM5020882 Total_RNA_Infected_K3_Rep2 treatment:HCMV infected, 24 hr, EU-labeled total RNA; rna type:steady state RNA; cell line:Kasumi-3 RNA-seq GSM5020883 Total_RNA_Infected_K3_Rep3 treatment:HCMV infected, 24 hr, EU-labeled total RNA; rna type:steady state RNA; cell line:Kasumi-3 RNA-seq GSM5020884 Total_RNA_Uninfected_K3_Rep1 treatment:mock-infected, 24 hr, EU-labeled total RNA; rna type:steady state RNA; cell line:Kasumi-3 RNA-seq GSM5020885 Total_RNA_Uninfected_K3_Rep2 treatment:mock-infected, 24 hr, EU-labeled total RNA; rna type:steady state RNA; cell line:Kasumi-3 RNA-seq GSM5020886 input_4hpi_Rep1 treatment:HCMV infected, 4 hr; chip antibody:none; cell line:Kasumi-3 ChIPseq GSM5020887 input_4hpi_Rep2 treatment:HCMV infected, 4 hr; chip antibody:none; cell line:Kasumi-3 ChIPseq GSM5020888 input_4hpi_Rep3 treatment:HCMV infected, 4 hr; chip antibody:none; cell line:Kasumi-3 ChIPseq GSM5020889 Pol2_4hpi_Rep1 treatment:HCMV infected, 24 hr; chip antibody:Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies); cell line:Kasumi-3 ChIPseq GSM5020890 Pol2_4hpi_Rep2 treatment:HCMV infected, 24 hr; chip antibody:Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies); cell line:Kasumi-3 ChIPseq GSM5020891 Pol2_4hpi_Rep3 treatment:HCMV infected, 24 hr; chip antibody:Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies); cell line:Kasumi-3 ChIPseq GSM5020892 H3K27Ac_4hpi_Rep1 treatment:HCMV infected, 4 hr; chip antibody:Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies); cell line:Kasumi-3 ChIPseq GSM5020893 H3K27Ac_4hpi_Rep2 treatment:HCMV infected, 4 hr; chip antibody:Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies); cell line:Kasumi-3 ChIPseq GSM5020894 H3K27Ac_4hpi_Rep3 treatment:HCMV infected, 4 hr; chip antibody:Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies); cell line:Kasumi-3 ChIPseq GSM5020910 input_mock_infected_cells_Rep1 treatment:live mock-infected, 24hpi; chip antibody:none; cell line:Kasumi-3 - GSM5020911 input_mock_infected_cells_Rep2 treatment:live mock-infected, 24hpi; chip antibody:none; cell line:Kasumi-3 - GSM5020912 Pol2_mock_infected_cells_Rep1 treatment:live mock-infected, 24hpi; chip antibody:Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies); cell line:Kasumi-3 - GSM5020913 Pol2_mock_infected_cells_Rep2 treatment:live mock-infected, 24hpi; chip antibody:Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies); cell line:Kasumi-3 - GSM5020914 input_24hpi_Rep1 treatment:live GFP+ infected, 24hpi; chip antibody:none; cell line:Kasumi-3 - GSM5020915 input_24hpi_Rep2 treatment:live GFP+ infected, 24hpi; chip antibody:none; cell line:Kasumi-3 - GSM5020916 Pol2_24hpi_Rep1 treatment:live GFP+ infected, 24hpi; chip antibody:Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies); cell line:Kasumi-3 - GSM5020917 Pol2_24hpi_Rep2 treatment:live GFP+ infected, 24hpi; chip antibody:Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies); cell line:Kasumi-3 - GSM5020839 24hr_K3_Uninfected_Sorted_Rep1 treatment:mock- infected, 24 hr; rna type:total RNA; cell line:Kasumi-3 RNA-seq GSM5020840 24hr_K3_Uninfected_Sorted_Rep2 treatment:mock- infected, 24 hr; rna type:total RNA; cell line:Kasumi-3 RNA-seq GSM5020841 24hr_K3_Infected_Sorted_Rep1 treatment:HCMV infected, 24 hr; rna type:total RNA; cell line:Kasumi-3 RNA-seq GSM5020842 24hr_K3_Infected_Sorted_Rep2 treatment:HCMV infected, 24 hr; rna type:total RNA; cell line:Kasumi-3 RNA-seq GSM5020895 H3K27me3_4hpi_Rep1 treatment:HCMV infected, 4 hr; chip antibody:Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies); cell line:Kasumi-3 ChIPseq GSM5020843 4hr_K3_Unsorted_Rep1 treatment:HCMV infected, 4 hr; rna type:total RNA; cell line:Kasumi-3 RNA-seq GSM5020844 4hr_K3_Unsorted_Rep2 treatment:HCMV infected, 4 hr; rna type:total RNA; cell line:Kasumi-3 RNA-seq GSM5020896 H3K27me3_4hpi_Rep2 treatment:HCMV infected, 4 hr; chip antibody:Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 (Cell Signaling Technologies); cell line:Kasumi-3 ChIPseq GSM5020845 4hr_K3_Unsorted_Rep3 treatment:HCMV infected, 4 hr; rna type:total RNA; cell line:Kasumi-3 RNA-seq
Platform	GPL18573   GPL20301   GPL24958   GPL29601   GPL29602   GPL29603  : Illumina NextSeq 500 (Homo sapiens);Illumina HiSeq 4000 (Homo sapiens);Illumina NextSeq 500 (Homo sapiens; Human betaherpesvirus 5);Illumina HiSeq 4000 (Human betaherpesvirus 5);Illumina NovaSeq 6000 (Human betaherpesvirus 5);Illumina HiSeq 4000 (Homo sapiens; Human betaherpesvirus 5)
Literature	33731453
Download	Download Genome Binding/Occupancy Profiling Analysis Data