Accession	GSE171518
Status	2022/12/21
Title	Role of the HCMV immediate early proteins in controlling the HCMV and cellular epigenomes
Organism	Homo sapiens
Experiment type	Genome binding/occupancy profiling by high throughput sequencing
Summary	Purpose: to investigate the role of the HCMV immediate early proteins in controlling the HCMV and cellular epigneomes during lytic infectioin
Samples	GSM ID Sample info Characteristics Description GSM5226523 H3K27Ac_Infected+shield_Input_Rep1 cell line:MRC-5 cells; infection:HHV5 TB40r-ddFKBP strain; treatment:infected +shield, 4 dpi; chip antibody:none input GSM5226524 H3K27Ac_Infected+shield_Input_Rep2 cell line:MRC-5 cells; infection:HHV5 TB40r-ddFKBP strain; treatment:infected +shield, 4 dpi; chip antibody:none input GSM5226525 H3K27Ac_Infected+shield_ChIP_Rep1 cell line:MRC-5 cells; infection:HHV5 TB40r-ddFKBP strain; treatment:infected +shield, 4 dpi; chip antibody:Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies) ChIPseq GSM5226526 H3K27Ac_Infected+shield_ChIP_Rep2 cell line:MRC-5 cells; infection:HHV5 TB40r-ddFKBP strain; treatment:infected +shield, 4 dpi; chip antibody:Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies) ChIPseq GSM5226527 PolII_Infected+shield_Input_Rep1 cell line:MRC-5 cells; infection:HHV5 TB40r-ddFKBP strain; treatment:infected +shield, 4 dpi; chip antibody:none input GSM5226528 PolII_Infected+shield_Input_Rep2 cell line:MRC-5 cells; infection:HHV5 TB40r-ddFKBP strain; treatment:infected +shield, 4 dpi; chip antibody:none input GSM5226529 PolII_Infected+shield_ChIP_Rep1 cell line:MRC-5 cells; infection:HHV5 TB40r-ddFKBP strain; treatment:infected +shield, 4 dpi; chip antibody:Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies) ChIPseq GSM5226530 PolII_Infected+shield_ChIP_Rep2 cell line:MRC-5 cells; infection:HHV5 TB40r-ddFKBP strain; treatment:infected +shield, 4 dpi; chip antibody:Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies) ChIPseq GSM5226531 H3K27Ac_Infected-shield_Input_Rep1 cell line:MRC-5 cells; infection:HHV5 TB40r-ddFKBP strain; treatment:infected no shield 4 dpi; chip antibody:none input GSM5226532 H3K27Ac_Infected-shield_Input_Rep2 cell line:MRC-5 cells; infection:HHV5 TB40r-ddFKBP strain; treatment:infected no shield 4 dpi; chip antibody:none input GSM5226533 H3K27Ac_Infected-shield_ChIP_Rep1 cell line:MRC-5 cells; infection:HHV5 TB40r-ddFKBP strain; treatment:infected no shield 4 dpi; chip antibody:Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies) ChIPseq GSM5226534 H3K27Ac_Infected-shield_ChIP_Rep2 cell line:MRC-5 cells; infection:HHV5 TB40r-ddFKBP strain; treatment:infected no shield 4 dpi; chip antibody:Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb #8173 (Cell Signaling Technologies) ChIPseq GSM5226535 PolII_Infected-shield_Input_Rep1 cell line:MRC-5 cells; infection:HHV5 TB40r-ddFKBP strain; treatment:infected no shield 4 dpi; chip antibody:none input GSM5226536 PolII_Infected-shield_Input_Rep2 cell line:MRC-5 cells; infection:HHV5 TB40r-ddFKBP strain; treatment:infected no shield 4 dpi; chip antibody:none input GSM5226537 PolII_Infected-shield_ChIP_Rep1 cell line:MRC-5 cells; infection:HHV5 TB40r-ddFKBP strain; treatment:infected no shield 4 dpi; chip antibody:Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies) ChIPseq GSM5226538 PolII_Infected-shield_ChIP_Rep2 cell line:MRC-5 cells; infection:HHV5 TB40r-ddFKBP strain; treatment:infected no shield 4 dpi; chip antibody:Rpb1 NTD (D8L4Y) Rabbit mAb #14958 (Cell Signaling Technologies) ChIPseq
Platform	GPL29977  : Illumina NovaSeq 6000 (Homo sapiens; Human betaherpesvirus 5)
Literature	36645269
Download	Download Genome Binding/Occupancy Profiling Analysis Data