Accession	GSE172194
Status	2021/5/8
Title	Epigenomic Regulation of Human T-Cell Leukemia Virus by Chromatin-Insulator CTCF
Organism	Homo sapiens
Experiment type	Genome binding/occupancy profiling by high throughput sequencing
Summary	We compared histone modifications of wild type and HTLV-1ΔCTCF provirus by ChIP sequencing and observed that loss of the vrial CTCF binding site in HTLV-1 results in increased histone methylation 5’ and 3’ of the CTCF site, suggesting that CTCF binding may act as a boundary element to epigenetic modification.
Samples	GSM ID Sample info Characteristics Description GSM5243704 JET-CTCF-K27 cell type:infected with HTLV-1-delta-CTCF; chip antibody:H3K36me3 (Cell Signaling, #4909) ChIP DNA GSM5243705 JET-CTCF-K36 cell type:infected with HTLV-1-delta-CTCF; chip antibody:H3K27me3 (Cell Signaling, #9733) ChIP DNA GSM5243706 JET-wt-Input cell type:infected with HTLV-1 wt; chip antibody:no antibody, input control input DNA GSM5243707 JET-p12-Input cell type:infected with HTLV-1p12stop; chip antibody:no antibody, input control input DNA GSM5243708 JET-CTCF-Input cell type:infected with HTLV-1-delta-CTCF; chip antibody:no antibody, input control input DNA GSM5243685 PBMC-wt-K4 cell type:HTLV-1 wt immortalized PBMCs; chip antibody:H3K4me3 (Cell Signaling, #9751) ChIP DNA GSM5243686 PBMC-wt-K36 cell type:HTLV-1 wt immortalized PBMCs; chip antibody:H3K36me3 (Cell Signaling, #4909) ChIP DNA GSM5243687 PBMC-wt-K27 cell type:HTLV-1 wt immortalized PBMCs; chip antibody:H3K27me3 (Cell Signaling, #9733) ChIP DNA GSM5243688 PBMC-p12stop-H3K4 cell type:HTLV-1p12stop immortalized PBMCs; chip antibody:H3K4me3 (Cell Signaling, #9751) ChIP DNA GSM5243689 PBMC-p12stop-K36 cell type:HTLV-1p12stop immortalized PBMCs; chip antibody:H3K36me3 (Cell Signaling, #4909) ChIP DNA GSM5243690 PBMC-p12stop-K27 cell type:HTLV-1p12stop immortalized PBMCs; chip antibody:H3K27me3 (Cell Signaling, #9733) ChIP DNA GSM5243691 PBMC-CTCF-H3K4 cell type:HTLV-1-delta-CTCF immortalized PBMCs; chip antibody:H3K4me3 (Cell Signaling, #9751) ChIP DNA GSM5243692 PBMC-CTCF-K36 cell type:HTLV-1-delta-CTCF immortalized PBMCs; chip antibody:H3K36me3 (Cell Signaling, #4909) ChIP DNA GSM5243693 PBMC-CTCF-K27 cell type:HTLV-1-delta-CTCF immortalized PBMCs; chip antibody:H3K27me3 (Cell Signaling, #9733) ChIP DNA GSM5243694 PBMC-wt-input cell type:HTLV-1 wt immortalized PBMCs; chip antibody:no antibody, input control input DNA GSM5243695 PBMC-p12stop-input cell type:HTLV-1p12stop immortalized PBMCs; chip antibody:no antibody, input control input DNA GSM5243696 PBMC-CTCF-input cell type:HTLV-1-delta-CTCF immortalized PBMCs; chip antibody:no antibody, input control input DNA GSM5243697 JET-wt-K4 cell type:infected with HTLV-1 wt; chip antibody:H3K4me3 (Cell Signaling, #9751) ChIP DNA GSM5243698 JET-wt-K27 cell type:infected with HTLV-1 wt; chip antibody:H3K36me3 (Cell Signaling, #4909) ChIP DNA GSM5243699 JET-wt-K36 cell type:infected with HTLV-1 wt; chip antibody:H3K27me3 (Cell Signaling, #9733) ChIP DNA GSM5243700 JET-p12-K4 cell type:infected with HTLV-1p12stop; chip antibody:H3K4me3 (Cell Signaling, #9751) ChIP DNA GSM5243701 JET-p12-K27 cell type:infected with HTLV-1p12stop; chip antibody:H3K36me3 (Cell Signaling, #4909) ChIP DNA GSM5243702 JET-p12-K36 cell type:infected with HTLV-1p12stop; chip antibody:H3K27me3 (Cell Signaling, #9733) ChIP DNA GSM5243703 JET-CTCF-K4 cell type:infected with HTLV-1-delta-CTCF; chip antibody:H3K4me3 (Cell Signaling, #9751) ChIP DNA
Platform	GPL30008  : Illumina HiSeq 3000 (Homo sapiens; Human T-cell leukemia virus type I)
Literature	34019588