Accession	GSE185235
Status	2023/6/20
Title	The HSV-1 ICP22 protein selectively impairs histone repositioning upon Pol II transcription downstream of genes (ATAC-seq I)
Organism	Homo sapiens
Experiment type	Genome binding/occupancy profiling by high throughput sequencing
Summary	Primary human fetal foreskin fibroblasts (HFFF) were infected with wild-type simplex virus 1 (HSV-1) strain F and null-ICP22 mutant at a multiplicity of infection (MOI) of 10. ATAC-seq libraries were prepared starting with 50,000 cells per condition following the protocol described by Buenrostro et al., Nature Methods 2013
Samples	GSM ID Sample info Characteristics Description GSM5608536 HFFF cells infected with ICP22-null mutant (12 hpi), replicate 1 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: 12 hpi;treatment: no treatment;agent: ICP22-null mutant of herpes simplex virus 1, strain F DNA isolated with MinElute PCR Purification Kit GSM5608537 HFFF cells infected with ICP22-null mutant (12 hpi), replicate 2 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: 12 hpi;treatment: no treatment;agent: ICP22-null mutant of herpes simplex virus 1, strain F DNA isolated with MinElute PCR Purification Kit GSM5608538 HFFF cells infected with ICP22-null mutant (8 hpi), replicate 1 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: 8 hpi;treatment: no treatment;agent: ICP22-null mutant of herpes simplex virus 1, strain F DNA isolated with MinElute PCR Purification Kit GSM5608539 HFFF cells infected with ICP22-null mutant (8 hpi), replicate 2 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: 8 hpi;treatment: no treatment;agent: ICP22-null mutant of herpes simplex virus 1, strain F DNA isolated with MinElute PCR Purification Kit GSM5608540 HFFF cells infected with ICP22-null mutant (12 hpi), treated with PAA, replicate 1 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: 12 hpi;treatment: PAA (350 µg/ml);agent: ICP22-null mutant of herpes simplex virus 1, strain F DNA isolated with MinElute PCR Purification Kit GSM5608541 HFFF cells infected with ICP22-null mutant (12 hpi), treated with PAA, replicate 2 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: 12 hpi;treatment: PAA (350 µg/ml);agent: ICP22-null mutant of herpes simplex virus 1, strain F DNA isolated with MinElute PCR Purification Kit GSM5608542 HFFF cells infected with ICP22-null mutant (8 hpi), treated with PAA, replicate 1 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: 8 hpi;treatment: PAA (350 µg/ml);agent: ICP22-null mutant of herpes simplex virus 1, strain F DNA isolated with MinElute PCR Purification Kit GSM5608543 HFFF cells infected with ICP22-null mutant (8 hpi), treated with PAA, replicate 2 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: 8 hpi;treatment: PAA (350 µg/ml);agent: ICP22-null mutant of herpes simplex virus 1, strain F DNA isolated with MinElute PCR Purification Kit GSM5608544 HFFF cells mock infected (12 hpi), replicate 1 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: no infection;treatment: no treatment;agent: no infection DNA isolated with MinElute PCR Purification Kit GSM5608545 HFFF cells mock infected (12 hpi), replicate 2 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: no infection;treatment: no treatment;agent: no infection DNA isolated with MinElute PCR Purification Kit GSM5608546 HFFF cells mock infected (12 hpi), treated with PAA, replicate 1 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: no infection;treatment: PAA (350 µg/ml);agent: no infection DNA isolated with MinElute PCR Purification Kit GSM5608547 HFFF cells mock infected (12 hpi), treated with PAA, replicate 2 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: no infection;treatment: PAA (350 µg/ml);agent: no infection DNA isolated with MinElute PCR Purification Kit GSM5608548 HFFF cells mock infected (8 hpi), replicate 2 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: no infection;treatment: no treatment;agent: no infection DNA isolated with MinElute PCR Purification Kit GSM5608549 HFFF cells mock infected (8 hpi), treated with PAA, replicate 2 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: no infection;treatment: PAA (350 µg/ml);agent: no infection DNA isolated with MinElute PCR Purification Kit GSM5608550 HFFF cells infected with HSV-1 WT (12 hpi), replicate 1 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: 12 hpi;treatment: no treatment;agent: Herpes simplex virus 1, strain F DNA isolated with MinElute PCR Purification Kit GSM5608551 HFFF cells infected with HSV-1 WT (12 hpi), replicate 2 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: 12 hpi;treatment: no treatment;agent: Herpes simplex virus 1, strain F DNA isolated with MinElute PCR Purification Kit GSM5608552 HFFF cells infected with HSV-1 WT (12 hpi), treated with PAA, replicate 1 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: 12 hpi;treatment: PAA (350 µg/ml);agent: Herpes simplex virus 1, strain F DNA isolated with MinElute PCR Purification Kit GSM5608553 HFFF cells infected with HSV-1 WT (12 hpi), treated with PAA, replicate 2 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: 12 hpi;treatment: PAA (350 µg/ml);agent: Herpes simplex virus 1, strain F DNA isolated with MinElute PCR Purification Kit GSM5608554 HFFF cells infected with HSV-1 WT (8 hpi), replicate 1 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: 8 hpi;treatment: no treatment;agent: Herpes simplex virus 1, strain F DNA isolated with MinElute PCR Purification Kit GSM5608555 HFFF cells infected with HSV-1 WT (8 hpi), replicate 2 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: 8 hpi;treatment: no treatment;agent: Herpes simplex virus 1, strain F DNA isolated with MinElute PCR Purification Kit GSM5608556 HFFF cells infected with HSV-1 WT (8 hpi), treated with PAA, replicate 1 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: 8 hpi;treatment: PAA (350 µg/ml);agent: Herpes simplex virus 1, strain F DNA isolated with MinElute PCR Purification Kit GSM5608557 HFFF cells infected with HSV-1 WT (8 hpi), treated with PAA, replicate 2 cell type: Primary human fetal foreskin fibroblasts (HFFF);time post infection: 8 hpi;treatment: PAA (350 µg/ml);agent: Herpes simplex virus 1, strain F DNA isolated with MinElute PCR Purification Kit
Platform	GPL18573  : Illumina NextSeq 500 (Homo sapiens)
Literature	37524699