Accession	GSE185238
Status	2023/6/20
Title	The HSV-1 ICP22 protein selectively impairs histone repositioning upon Pol II transcription downstream of genes (Omni-ATAC-seq I)
Organism	Homo sapiens
Experiment type	Genome binding/occupancy profiling by high throughput sequencing
Summary	T-HF cells were grown either in the presence or absence of DOX. Upon DOX exposure, cells expressed either HA-ICP22 or HA-ICP22 and V5-ICP27. T-HF HA-ICP22 cells were treated with salt stress for 2 hours before proceeding with Omni-ATAC-seq. OMNI-ATAC-seq was conducted for the KOS1.1 strain and compared to a full US1 deletion mutant.
Samples	GSM ID Sample info Characteristics Description GSM5608600 HA-ICP22+V5-ICP27 cells, DOX, replicate 1 cell type: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;treatment: DOX (5 μg/mL);treatment 2: no treatment DNA isolated with MinElute PCR Purification Kit GSM5608601 HA-ICP22+V5-ICP27 cells, no DOX, replicate 1 cell type: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;treatment: no treatment;treatment 2: no treatment DNA isolated with MinElute PCR Purification Kit GSM5608602 HA-ICP22+V5-ICP27 cells, DOX, replicate 2 cell type: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;treatment: DOX (5 μg/mL);treatment 2: no treatment DNA isolated with MinElute PCR Purification Kit GSM5608603 HA-ICP22+V5-ICP27 cells, no DOX, replicate 2 cell type: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;treatment: no treatment;treatment 2: no treatment DNA isolated with MinElute PCR Purification Kit GSM5608604 HA-ICP22 cells, salt stress, DOX, replicate 1 cell type: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;treatment: DOX (5 μg/mL);treatment 2: 80 mM KCl DNA isolated with MinElute PCR Purification Kit GSM5608605 HA-ICP22 cells, salt stress, no DOX, replicate 1 cell type: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;treatment: no treatment;treatment 2: 80 mM KCl DNA isolated with MinElute PCR Purification Kit GSM5608606 HA-ICP22 cells, salt stress, DOX, replicate 2 cell type: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;treatment: DOX (5 μg/mL);treatment 2: 80 mM KCl DNA isolated with MinElute PCR Purification Kit GSM5608607 HA-ICP22 cells, salt stress, no DOX, replicate 2 cell type: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;treatment: no treatment;treatment 2: 80 mM KCl DNA isolated with MinElute PCR Purification Kit GSM5608608 primary HFFF cells, Mock-infected, PAA 350ug/ml, replicate 1 cell type: primary Human Foetal Foreskin Fibroblast cells;treatment: 350 ug'/ml PAA;treatment 2: Mock DNA isolated with MinElute PCR Purification Kit GSM5608609 primary HFFF cells, Mock infected, PAA 350ug/ml, replicate 2 cell type: primary Human Foetal Foreskin Fibroblast cells;treatment: 350 ug'/ml PAA;treatment 2: Mock DNA isolated with MinElute PCR Purification Kit GSM5608610 primary HFFF cells, infected with KOS 1.1 strain, PAA 350ug/ml, replicate 1 cell type: primary Human Foetal Foreskin Fibroblast cells;treatment: 350 ug'/ml PAA;treatment 2: WT KOS 1.1 DNA isolated with MinElute PCR Purification Kit GSM5608611 primary HFFF cells, infected with KOS 1.1 strain, PAA 350ug/ml, replicate 2 cell type: primary Human Foetal Foreskin Fibroblast cells;treatment: 350 ug'/ml PAA;treatment 2: WT KOS 1.1 DNA isolated with MinElute PCR Purification Kit GSM5608612 primary HFFF cells, infected with KOS 1.1 strain derived US1 knock-out, PAA 350ug/ml, replicate 1 cell type: primary Human Foetal Foreskin Fibroblast cells;treatment: 350 ug'/ml PAA;treatment 2: KOS 1.1 with US1 deletion DNA isolated with MinElute PCR Purification Kit GSM5608613 primary HFFF cells, infected with KOS 1.1 strain derived US1 knock-out, PAA 350ug/ml, replicate 2 cell type: primary Human Foetal Foreskin Fibroblast cells;treatment: 350 ug'/ml PAA;treatment 2: KOS 1.1 with US1 deletion DNA isolated with MinElute PCR Purification Kit
Platform	GPL18573  : Illumina NextSeq 500 (Homo sapiens)
Literature	37524699