Accession	GSE185240
Status	2023/6/20
Title	The HSV-1 ICP22 protein selectively impairs histone repositioning upon Pol II transcription downstream of genes (Omni-ATAC-seq III)
Organism	Homo sapiens
Experiment type	Genome binding/occupancy profiling by high throughput sequencing
Summary	We report that HSV-1 gives rise to transposase accessible chromatin in gene downstream areas, in regions that show transcription termination defects. We show that the viral protein ICP22 is responsible for this open chromatin.
Samples	GSM ID Sample info Characteristics Description GSM5608644 GFP-expressing HH-TERT cells, infected with US1 deletion mutant HSV-1 strain 17, 8 hpi, replicate 1 cell line: HFF-TERT cells doxycycline-inducibly expressing GFP (control sample);virus strain: HSV-1(17+)Lox BAC derived US1 deletion mutant;agent: infected with US1 mutant - GSM5608645 GFP-expressing HH-TERT cells, infected with US1 deletion mutant HSV-1 strain 17, 8 hpi, replicate 2 cell line: HFF-TERT cells doxycycline-inducibly expressing GFP (control sample);virus strain: HSV-1(17+)Lox BAC derived US1 deletion mutant;agent: infected with US1 mutant - GSM5608646 GFP-expressing HH-TERT cells, mock-infected, 8 hpi, replicate 1 cell line: HFF-TERT cells doxycycline-inducibly expressing GFP (control sample);virus strain: N/A;agent: mock infected - GSM5608647 GFP-expressing HH-TERT cells, mock-infected, 8 hpi, replicate 2 cell line: HFF-TERT cells doxycycline-inducibly expressing GFP (control sample);virus strain: N/A;agent: mock infected - GSM5608648 GFP-expressing HH-TERT cells, infected with BAC-derived WT HSV-1 strain 17, 8 hpi, replicate 1 cell line: HFF-TERT cells doxycycline-inducibly expressing GFP (control sample);virus strain: HSV-1(17+)Lox BAC derived WT virus;agent: infected with parental BAC derived wt virus - GSM5608649 GFP-expressing HH-TERT cells, infected with BAC-derived WT HSV-1 strain 17, 8 hpi, replicate 2 cell line: HFF-TERT cells doxycycline-inducibly expressing GFP (control sample);virus strain: HSV-1(17+)Lox BAC derived WT virus;agent: infected with parental BAC derived wt virus -
Platform	GPL18573  : Illumina NextSeq 500 (Homo sapiens)
Literature	37524699