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Dataset Information
| Accession |
GSE197461
|
| Status |
2023/12/1 |
| Title |
Decipher heterogeneity of cervical squamous cell carcinoma and adenocarcinoma with different HPV status: combining scRNA and TCR-seq with 3D organoid |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing;Other |
| Summary |
Although adenocarcinoma of the cervix (ADC) exhibits a more malignant phenotype and poorer prognosis than squamous cell carcinoma (SCC), they are treated identically. This clinical dilemma calls for deeper investigation into differences between SCC and ADC. We studied 3 human papillomavirus (HPV)-positive SCC, 3 HPV-associated (HPVA) and 2 non-HPV-associated (NHPVA) ADC samples with single-cell RNA and T cell receptor sequencing plus 3D organoid verification. Notably, we revealed the malignant origin of epithelial cells characterized by stemness and poor differentiation and the potential mechanism of HPVA and NHPVA oncogenesis, deciphering differences between SCC and ADC. Additionally, we discovered the key role of tip cells in sprouting angiogenesis, the protumoral effects of cancer-associated fibroblasts and the functional dysregulation of T lymphocytes. We highlighted tumor microenvironment differences between different histologic subtypes through cellular interaction analysis. Importantly, we discovered novel precise treatment strategies specifically for HPV-positive SCC, HPVA and NHPVA-ADC, providing tremendous clinical value. |
| Samples |
| GSM ID |
Sample info |
Characteristics |
Description |
|
GSM5917941
|
ADC_2, scRNA-seq |
disease state:cervical cancer; tissue:cervical cancer tissue; histologic type:ADC; hpv infection:positive; hpv subtype:HPV18; library:10x Genomics 5' GEX library |
- |
|
GSM5917942
|
ADC_3, scRNA-seq |
disease state:cervical cancer; tissue:cervical cancer tissue; histologic type:ADC; hpv infection:positive; hpv subtype:HPV45; library:10x Genomics 5' GEX library |
- |
|
GSM5917943
|
ADC_4, scRNA-seq |
disease state:cervical cancer; tissue:cervical cancer tissue; histologic type:ADC; hpv infection:negative; hpv subtype:not applicable; library:10x Genomics 5' GEX library |
- |
|
GSM5917944
|
ADC_5, scRNA-seq |
disease state:cervical cancer; tissue:cervical cancer tissue; histologic type:ADC; hpv infection:negative; hpv subtype:not applicable; library:10x Genomics 5' GEX library |
- |
|
GSM5917945
|
SCC_1, TCR-seq |
disease state:cervical cancer; tissue:cervical cancer tissue; histologic type:SCC; hpv infection:positive; hpv subtype:HPV 58; library:10x Genomics TCR library |
- |
|
GSM5917946
|
SCC_2, TCR-seq |
disease state:cervical cancer; tissue:cervical cancer tissue; histologic type:SCC; hpv infection:positive; hpv subtype:HPV 16; library:10x Genomics TCR library |
- |
|
GSM5917947
|
SCC_3, TCR-seq |
disease state:cervical cancer; tissue:cervical cancer tissue; histologic type:SCC; hpv infection:positive; hpv subtype:HPV 16; library:10x Genomics TCR library |
- |
|
GSM5917948
|
ADC_1, TCR-seq |
disease state:cervical cancer; tissue:cervical cancer tissue; histologic type:ADC; hpv infection:positive; hpv subtype:HPV16; library:10x Genomics TCR library |
- |
|
GSM5917949
|
ADC_2, TCR-seq |
disease state:cervical cancer; tissue:cervical cancer tissue; histologic type:ADC; hpv infection:positive; hpv subtype:HPV18; library:10x Genomics TCR library |
- |
|
GSM5917950
|
ADC_3, TCR-seq |
disease state:cervical cancer; tissue:cervical cancer tissue; histologic type:ADC; hpv infection:positive; hpv subtype:HPV45; library:10x Genomics TCR library |
- |
|
GSM5917951
|
ADC_4, TCR-seq |
disease state:cervical cancer; tissue:cervical cancer tissue; histologic type:ADC; hpv infection:negative; hpv subtype:not applicable; library:10x Genomics TCR library |
- |
|
GSM5917952
|
ADC_5, TCR-seq |
disease state:cervical cancer; tissue:cervical cancer tissue; histologic type:ADC; hpv infection:negative; hpv subtype:not applicable; library:10x Genomics TCR library |
- |
|
GSM5917937
|
SCC_1, scRNA-seq |
disease state:cervical cancer; tissue:cervical cancer tissue; histologic type:SCC; hpv infection:positive; hpv subtype:HPV 58; library:10x Genomics 5' GEX library |
- |
|
GSM5917938
|
SCC_2, scRNA-seq |
disease state:cervical cancer; tissue:cervical cancer tissue; histologic type:SCC; hpv infection:positive; hpv subtype:HPV 16; library:10x Genomics 5' GEX library |
- |
|
GSM5917939
|
SCC_3, scRNA-seq |
disease state:cervical cancer; tissue:cervical cancer tissue; histologic type:SCC; hpv infection:positive; hpv subtype:HPV 16; library:10x Genomics 5' GEX library |
- |
|
GSM5917940
|
ADC_1, scRNA-seq |
disease state:cervical cancer; tissue:cervical cancer tissue; histologic type:ADC; hpv infection:positive; hpv subtype:HPV16; library:10x Genomics 5' GEX library |
- |
|
| Platform |
GPL24676 : Illumina NovaSeq 6000 (Homo sapiens) |
| Literature |
37794698 |
| Download |
Download scRNA Analysis Data
|
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scRNA-seq UMAP
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Virus Dataset Detail
