Accession	GSE208077
Status	2023/6/20
Title	The HSV-1 ICP22 protein selectively impairs histone repositioning upon Pol II transcription downstream of genes (OMNI-ATAC-seq IV)
Organism	Homo sapiens
Experiment type	Genome binding/occupancy profiling by high throughput sequencing
Summary	Human telomerase reverse transcriptase (hTERT)-Immortalized cell line (T-HF) inducibly expressing ICP22 or ICP27 (doxycycline-induced) were analysed for downstream open chromation region (dOCR) formation. ICP22 or ICP27 expression was induced for 24 h prior to other treatments or harvest, respectively. ICP22 expressing cells were additionally treated with KCl (200 mM) for 2 h prior to harvest. OMNI-ATACseq libraries were prepared with 50000 cells/sample as described by Corces, M., Trevino, A., Hamilton, E. et al. An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues. Nat Methods 14, 959–962 (2017). https://doi.org/10.1038/nmeth.4396
Samples	GSM ID Sample info Characteristics Description GSM6336802 HFF-tert, doxycycline-inducible ICP22, 5ug/ml doxycycline for 24h, 200mM KCl for 2h, rep1 tissue: foreskin;cell line: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;transduction: lentivirus containing an inducible ICP22 cassette and rtTA3;treatment: 5ug/ml doxycycline for 24h, 200mM KCl for 2h ICP22_dox_KCl_R1 GSM6336803 HFF-tert, doxycycline-inducible ICP22, 5ug/ml doxycycline for 24h, 200mM KCl for 2h, rep2 tissue: foreskin;cell line: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;transduction: lentivirus containing an inducible ICP22 cassette and rtTA3;treatment: 5ug/ml doxycycline for 24h, 200mM KCl for 2h ICP22_dox_KCl_R2 GSM6336804 HFF-tert, doxycycline-inducible ICP22, 5ug/ml doxycycline for 24h, no KCl, rep1 tissue: foreskin;cell line: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;transduction: lentivirus containing an inducible ICP22 cassette and rtTA3;treatment: 5ug/ml doxycycline for 24h, no KCl ICP22_dox_R1 GSM6336805 HFF-tert, doxycycline-inducible ICP22, 5ug/ml doxycycline for 24h, no KCl, rep2 tissue: foreskin;cell line: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;transduction: lentivirus containing an inducible ICP22 cassette and rtTA3;treatment: 5ug/ml doxycycline for 24h, no KCl ICP22_dox_R2 GSM6336806 HFF-tert, doxycycline-inducible ICP22, no doxycycline, 200mM KCl for 2h, rep1 tissue: foreskin;cell line: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;transduction: lentivirus containing an inducible ICP22 cassette and rtTA3;treatment: no doxycycline, 200mM KCl for 2h ICP22_no_dox_KCl_R1 GSM6336807 HFF-tert, doxycycline-inducible ICP22, no doxycycline, 200mM KCl for 2h, rep2 tissue: foreskin;cell line: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;transduction: lentivirus containing an inducible ICP22 cassette and rtTA3;treatment: no doxycycline, 200mM KCl for 2h ICP22_no_dox_KCl_R2 GSM6336808 HFF-tert, doxycycline-inducible ICP22, no doxycycline, no KCl, rep1 tissue: foreskin;cell line: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;transduction: lentivirus containing an inducible ICP22 cassette and rtTA3;treatment: no doxycycline, no KCl ICP22_no_dox_R1 GSM6336809 HFF-tert, doxycycline-inducible ICP22, no doxycycline, no KCl, rep2 tissue: foreskin;cell line: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;transduction: lentivirus containing an inducible ICP22 cassette and rtTA3;treatment: no doxycycline, no KCl ICP22_no_dox_R2 GSM6336810 HFF-tert, doxycycline-inducible ICP27, 5ug/ml doxycycline for 24h, no KCl, rep1 tissue: foreskin;cell line: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;transduction: lentivirus containing an inducible ICP27 cassette and rtTA3;treatment: 5ug/ml doxycycline for 24h, no KCl ICP27_dox_R1 GSM6336811 HFF-tert, doxycycline-inducible ICP27, 5ug/ml doxycycline for 24h, no KCl, rep2 tissue: foreskin;cell line: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;transduction: lentivirus containing an inducible ICP27 cassette and rtTA3;treatment: 5ug/ml doxycycline for 24h, no KCl ICP27_dox_R2 GSM6336812 HFF-tert, doxycycline-inducible ICP27, no treatment, rep1 tissue: foreskin;cell line: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;transduction: lentivirus containing an inducible ICP27 cassette and rtTA3;treatment: no treatment ICP27_no_dox_R1 GSM6336813 HFF-tert, doxycycline-inducible ICP27, no treatment, rep2 tissue: foreskin;cell line: Human telomerase reverse transcriptase (hTERT)-Immortalized cell line;transduction: lentivirus containing an inducible ICP27 cassette and rtTA3;treatment: no treatment ICP27_no_dox_R2
Platform	GPL18573  : Illumina NextSeq 500 (Homo sapiens)
Literature	37524699