Accession	GSE220859
Status	2023/2/20
Title	HIV-1 integration in microglia (ChIP-seq of CTCF-KD/-NT)
Organism	Homo sapiens
Experiment type	Genome binding/occupancy profiling by high throughput sequencing
Summary	We performed ChIP-seq in a microglia cell line (C20) upon CTCF-KD and CTCF-NT (wild-type) to understand the impact of CTCF loss on chromatin and HIV-1 integration pattern in the C20 cell line.
Samples	GSM ID Sample info Characteristics Description GSM6824674 Microglia H3K36me3 CTCF-KD rep1 cell line:C20; chip antibody:H3K36me3 (ab9050, Abcam); treatment:CTCF-KD Sequenced on Nov 2022 GSM6824675 Microglia H3K36me3 CTCF-KD rep2 cell line:C20; chip antibody:H3K36me3 (ab9050, Abcam); treatment:CTCF-KD Sequenced on Nov 2022 GSM6824676 Microglia H3K36me3 NT rep1 cell line:C20; chip antibody:H3K36me3 (ab9050, Abcam); treatment:untreated Sequenced on Nov 2022 GSM6824677 Microglia H3K36me3 NT rep2 cell line:C20; chip antibody:H3K36me3 (ab9050, Abcam); treatment:untreated Sequenced on Nov 2022 GSM6824678 Microglia CTCF NT rep1 cell line:C20; chip antibody:CTCF (Cell Signaling, #3418); treatment:untreated Sequenced on Nov 2022 GSM6824679 Microglia CTCF NT rep2 cell line:C20; chip antibody:CTCF (Cell Signaling, #3418); treatment:untreated Sequenced on Nov 2022 GSM6824680 Microglia CTCF NT rep3 cell line:C20; chip antibody:CTCF (Cell Signaling, #3418); treatment:untreated Sequenced on Nov 2022 GSM6824681 Microglia CTCF CTCF-KD rep1 cell line:C20; chip antibody:CTCF (Cell Signaling, #3418); treatment:CTCF-KD Sequenced on Nov 2022 GSM6824682 Microglia CTCF CTCF-KD rep2 cell line:C20; chip antibody:CTCF (Cell Signaling, #3418); treatment:CTCF-KD Sequenced on Nov 2022 GSM6824683 Microglia Input rep1 cell line:C20; chip antibody:None; treatment:untreated Sequenced on Nov 2022 GSM6824684 Microglia Input rep2 cell line:C20; chip antibody:None; treatment:untreated Sequenced on Nov 2022
Platform	GPL24676  : Illumina NovaSeq 6000 (Homo sapiens)
Literature	36790927
Download	Download Genome Binding/Occupancy Profiling Analysis Data