Virus Dataset Detail

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Dataset Information
Accession GSE221680
Status 2023/2/28
Title Whole transcriptome analysis after JEV infection in SH-SY5Y
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Infection by neurotropic virus Japanese Encephalitis Virus (JEV) is characterized by profound neuronal cell death and neuroinflammation. Long non-coding RNAs (lncRNAs) are critical regulatory players in diverse biological processes, including viral pathogenesis. We use whole transcriptomic sequencing to identify a lncRNA JINR1 (JEV-induced non-coding RNA 1) induced upon JEV infection in neuronal cells. Transcription factor NF-κB triggers JINR-1 expression during JEV infection. Loss of JINR-1 impairs virus replication and reduces JEV-induced neuronal cell death and expression of genes involved in ER stress and neuroinflammation. Mechanistically, JINR1 inhibits the expression of mir-216-5p, which inhibits host factors GRP78 and c-JUN and the viral genome. In line with its role as a pro-viral host factor, JINR1 interacts with RBM10 and NF-κB to promote the expression of genes involved in ER stress and neuroinflammation. Our results suggest a role for JINR-1 in promoting JEV-induced cell death and neuroinflammation.
Samples
GSM ID Sample info Characteristics Description
GSM6893020 hNeuronal cells, Control (C1), 48h tissue:Brain; cell line:SH-SY5Y; cell type:Neuronal; genotype:WT; treatment:Mock infected -
GSM6893021 hNeuronal cells, Control (C2), 48h tissue:Brain; cell line:SH-SY5Y; cell type:Neuronal; genotype:WT; treatment:Mock infected -
GSM6893022 hNeuronal cells, Control (C3), 48h tissue:Brain; cell line:SH-SY5Y; cell type:Neuronal; genotype:WT; treatment:Mock infected -
GSM6893023 hNeuronal cells, JEV treated (I1), 48h tissue:Brain; cell line:SH-SY5Y; cell type:Neuronal; genotype:WT; treatment:JEV infected (5 MOI) -
GSM6893024 hNeuronal cells, JEV treated (I2), 48h tissue:Brain; cell line:SH-SY5Y; cell type:Neuronal; genotype:WT; treatment:JEV infected (5 MOI) -
GSM6893025 hNeuronal cells, JEV treated (I3), 48h tissue:Brain; cell line:SH-SY5Y; cell type:Neuronal; genotype:WT; treatment:JEV infected (5 MOI) -
Platform GPL16791  : Illumina HiSeq 2500 (Homo sapiens)
Literature 37991381  
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xCell Plot
* p<0.05 ** p<0.01