Accession	GSE221686
Status	2024/2/1
Title	The latent HIV reservoir in the brain of people with HIV under antiretroviral therapy (scATAC-Seq)
Organism	Homo sapiens
Experiment type	Genome binding/occupancy profiling by high throughput sequencing
Summary	A growing body of data suggests that the human brain serves as a sanctuary for HIV persistence despite life-long antiretroviral therapy. Microglia, the innate immune cells of the brain parenchyma,  may serve as a reservoir for rebound of HIV infection. The extent of the latent brain reservoir and molecular phenotype of HIV infected microglia cells, however, are unknown. To address this major knowledge gap, we leveraged the ‘Last Gift’ rapid autopsy cohort to perform a multi-omics approach (single cell RNA-seq, single cell ATAC-seq, and H3K27ac ChIP-seq) of the myeloid compartment creating a gene expression and chromatin accessibility atlas of human microglia isolated from three male individuals with HIV on suppressive antiretroviral therapy.
Samples	GSM ID Sample info Characteristics Description GSM6893080 LG05 cell type: CD45+ cells;tissue: Frontal cortex (BA9);strain: Male;age: 57 years 10x Genomics GSM6893081 LG14 cell type: CD45+ cells;tissue: Frontal cortex (BA9);strain: Male;age: 59 years 10x Genomics GSM6893082 LG15 cell type: CD45+ cells;tissue: Frontal cortex (BA9);strain: Male;age: 88 years 10x Genomics
Platform	GPL24676  : Illumina NovaSeq 6000 (Homo sapiens)
Literature