| Accession |
GSE228078
|
| Status |
2023/9/5 |
| Title |
Single cell transcriptomic atlas of human plasmacytoid dendritic cells reveals a distinct cytotoxic-like subset in HIV-1 infected individuals |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing |
| Summary |
To understand how the transcriptome of pDCs is impacted by HIV-1 infection and exogenous stimulation, we sorted pDCs from people with HIV-1 (PWH) on antiretroviral treatment before and after toll-like receptor 9 (TLR9) agonist treatment, as well as from healthy controls, and performed single-cell (sc)-RNA sequencing. We generated a transcriptomic atlas of over 112,000 pDCs. Our cluster analysis revealed an inducible cytotoxic-like pDC cluster characterized by high expression of both antiviral and cytotoxic genes. We further reported that this cytotoxic-like pDC cluster is distinct from NK and T cells. This observation was further supported by our cell-cell communication analysis revealing that cytotoxic-like pDCs exhibit similar incoming and outgoing cellular communicating signals as other pDCs. In addition to the cytotoxic-like pDC cluster, we also characterized two homogenous pDC clusters (pDC1 and pDC2) and an exhausted pDC cluster. In conclusion, our findings provide a comprehensive pDC transcriptomic atlas and open a new scope on the mechanisms of regulation and function of cytotoxic-like pDCs in HIV and other infections. |
| Samples |
| GSM ID |
Sample info |
Characteristics |
Description |
|
GSM7113581
|
HC61 |
cell type:PBMC |
10x Genomics |
|
GSM7113582
|
HC63 |
cell type:PBMC |
10x Genomics |
|
GSM7113583
|
HC65 |
cell type:PBMC |
10x Genomics |
|
GSM7113584
|
HC66 |
cell type:PBMC |
10x Genomics |
|
GSM7113585
|
HIV103_V12 |
cell type:PBMC |
10x Genomics |
|
GSM7113586
|
HIV103_V1 |
cell type:PBMC |
10x Genomics |
|
GSM7113587
|
HIV109_V12 |
cell type:PBMC |
10x Genomics |
|
GSM7113588
|
HIV109_V1 |
cell type:PBMC |
10x Genomics |
|
GSM7113589
|
HIV112_V12 |
cell type:PBMC |
10x Genomics |
|
GSM7113590
|
HIV112_V1 |
cell type:PBMC |
10x Genomics |
|
GSM7113591
|
HIV113_V12 |
cell type:PBMC |
10x Genomics |
|
GSM7113592
|
HIV113_V1 |
cell type:PBMC |
10x Genomics |
|
| Platform |
GPL24676 : Illumina NovaSeq 6000 (Homo sapiens) |
| Literature |
37664600 38133956 |
| Download |
Download scRNA Analysis Data
|
|
scRNA-seq UMAP
|
|
Virus Dataset Detail
