Accession	GSE240450
Status	2023/8/9
Title	Hepatitis B virus X protein contributes to hepatocellular carcinoma via upregulation of KIAA1429 methyltransferase and mRNA m6A hypermethylation of HSPG2/Perlecan [MeRIP-Seq]
Organism	Homo sapiens
Experiment type	Methylation profiling by high throughput sequencing
Summary	Chronic hepatitis B virus (HBV) remains to be the most common risk factor of hepatocellular carcinoma (HCC). While work has primarily focussed on understanding the direct and indirect mechanisms of Hepatitis B virus X protein (HBx) mediated hepatocarcinogenesis, from genetic and epigenetic perspectives, its influence on RNA modification mediated onset of liver malignancies is less well understood. This study explored the role of HBV-encoded HBx in altering the m6A methylome profile and its implications on the pathogenesis of HCC. We established HBx expressing stable HCC cell lines, Huh7-HBx and HepG2-HBx, and explored the transcriptomic and epitranscriptomic profiles by RNA-seq and MeRIP-seq, respectively. Preliminary results suggest that HBx promotes liver cell proliferation, migration, survival and overall m6A methylation in HCC cells and is involved in modulating the extracellular matrix. We show that HBx mediates liver cell transformation by upregulating KIAA1429 methyltransferase. HBx also drives the expression and hypermethylation of the extracellular matrix protein HSPG2/Perlecan and promotes tumourigenesis. Our findings indicate a potential interaction between KIAA1429 and HSPG2, thus could be novel targets in combating HBx-driven HCC and warrants further investigation.
Samples	GSM ID Sample info Characteristics Description GSM7698148 Huh7-HBx cells with Doxycycline treatment, m6A enriched cell line:Huh7; cell type:Liver cancer cells; genotype:IP; treatment:With HBx - GSM7698145 Huh7-HBx cells without Doxycycline treatment, Input control cell line:Huh7; cell type:Liver cancer cells; genotype:Input; treatment:Without HBx - GSM7698146 Huh7-HBx cells without Doxycycline treatment, m6A enriched cell line:Huh7; cell type:Liver cancer cells; genotype:IP; treatment:Without HBx - GSM7698147 Huh7-HBx cells with Doxycycline treatment, Input control cell line:Huh7; cell type:Liver cancer cells; genotype:Input; treatment:With HBx -
Platform	GPL11154  : Illumina HiSeq 2000 (Homo sapiens)
Literature