Virus Dataset Detail

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Dataset Information
Accession GSE242170
Status 2023/9/12
Title IFN-λ signaling protects mice from severe HSV-1 skin disease II
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Type III interferons (IFN-λ) are antiviral and immunomodulatory cytokines that have been best characterized in respiratory and gastrointestinal infections, but the effects of IFN-λ against skin infections have not been extensively investigated. We sought to define the skin-specific effects of IFN-λ against the highly prevalent human pathogen herpes simplex virus (HSV). We infected mice lacking the IFN-λ receptor (Ifnlr1-/-), both the IFN-λ and the IFN-αβ receptor (Ifnar1-/- Ifnlr1-/-), or IFN-λ cytokines (Ifnl2/3-/-) and found that IFN-λ restricts the severity of HSV-1 and HSV-2 skin lesions, independent of a direct effect on viral load. Using conditional knockout mice, we found that IFN-λ signaling in both keratinocytes and neutrophils was necessary to control HSV-1 skin lesion severity, and that IFN-λ signaling in keratinocytes suppressed CXCL9-mediated neutrophil recruitment to the skin. Furthermore, depleting neutrophils prevented the development of severe HSV-1 skin lesions in Ifnlr1-/- mice. Altogether, our results suggest that IFN-λ plays an immunomodulatory role in the skin that restricts neutrophil-mediated pathology during HSV infection, and suggest potential applications for IFN-λ in treating viral skin infections.
Samples
GSM ID Sample info Characteristics Description
GSM7750302 A549_RenLuc_Media_1 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:RenLuc (WT); treatment:Media -
GSM7750303 A549_RenLuc_Media_2 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:RenLuc (WT); treatment:Media -
GSM7750304 A549_RenLuc_Media_3 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:RenLuc (WT); treatment:Media -
GSM7750305 A549_RenLuc_IFNL_1 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:RenLuc (WT); treatment:IFN-lamda -
GSM7750306 A549_RenLuc_IFNL_2 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:RenLuc (WT); treatment:IFN-lamda -
GSM7750307 A549_RenLuc_IFNL_3 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:RenLuc (WT); treatment:IFN-lamda -
GSM7750308 A549_RenLuc_IFNB_1 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:RenLuc (WT); treatment:IFN-beta -
GSM7750309 A549_RenLuc_IFNB_2 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:RenLuc (WT); treatment:IFN-beta -
GSM7750310 A549_RenLuc_IFNB_3 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:RenLuc (WT); treatment:IFN-beta -
GSM7750311 A549_IFNLRKO_Media_1 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:IFNLR1 KO; treatment:Media -
GSM7750312 A549_IFNLRKO_Media_2 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:IFNLR1 KO; treatment:Media -
GSM7750313 A549_IFNLRKO_Media_3 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:IFNLR1 KO; treatment:Media -
GSM7750314 A549_IFNLRKO_IFNB_1 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:IFNLR1 KO; treatment:IFN-beta -
GSM7750315 A549_IFNLRKO_IFNB_2 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:IFNLR1 KO; treatment:IFN-beta -
GSM7750316 A549_IFNLRKO_IFNB_3 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:IFNLR1 KO; treatment:IFN-beta -
GSM7750317 A549_IFNARKO_Media_1 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:IFNAR1KO; treatment:Media -
GSM7750318 A549_IFNARKO_Media_2 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:IFNAR1KO; treatment:Media -
GSM7750319 A549_IFNARKO_Media_3 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:IFNAR1KO; treatment:Media -
GSM7750320 A549_IFNARKO_IFNL_1 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:IFNAR1KO; treatment:IFN-lamda -
GSM7750321 A549_IFNARKO_IFNL_2 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:IFNAR1KO; treatment:IFN-lamda -
GSM7750322 A549_IFNARKO_IFNL_3 tissue:Lung adenocarcinoma; cell line:A549; cell type:epithelial; genotype:IFNAR1KO; treatment:IFN-lamda -
Platform GPL24676  : Illumina NovaSeq 6000 (Homo sapiens)
Literature 38426749  
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* p<0.05 ** p<0.01