Virus Dataset Detail

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Dataset Information
Accession GSE246082
Status 2023/10/25
Title Dysregulated neuroimmune interactions and sustained type I interferon signaling after human immunodeficiency virus type 1 infection of human iPSC derived microglia and cerebral organoids
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Human immunodeficiency virus type-1 (HIV-1) associated neurocognitive disorder (HAND) affects up to half of HIV-1 positive patients with long term neurological consequences, including dementia. There are no effective therapeutics for HAND because the pathophysiology of HIV-1 induced glial and neuronal functional deficits in humans remains enigmatic. To bridge this knowledge gap, we established a model simulating HIV-1 infection in the central nervous system using human induced pluripotent stem cell (iPSC) derived microglia combined with sliced neocortical organoids. Upon incubation with two replication-competent macrophage-tropic HIV-1 strains (JRFL and YU2), we observed that microglia not only became productively infected but also exhibited inflammatory activation. RNA sequencing revealed a significant and sustained activation of type I interferon signaling pathways. Incorporating microglia into sliced neocortical organoids extended the effects of aberrant type I interferon signaling in a human neural context. Collectively, our results illuminate the role of persistent type I interferon signaling in HIV-1 infected microglial in a human neural model, suggesting its potential significance in the pathogenesis of HAND.
Samples
GSM ID Sample info Characteristics Description
GSM7855211 Control No Treatment 1 Day 15 time:15 days; cell type:Microglia; treatment:Mock-infected Control iPSC-derived microglial culture, mock-infected
GSM7855212 Control No Treatment 2 Day 15 time:15 days; cell type:Microglia; treatment:Mock-infected Control iPSC-derived microglial culture, mock-infected
GSM7855213 Control No Treatment 3 Day 15 time:15 days; cell type:Microglia; treatment:Mock-infected Control iPSC-derived microglial culture, mock-infected
GSM7855214 HIV JR-FL 1 Day 15 time:15 days; cell type:Microglia; treatment:HIV JR-FL Infected iPSC-derived microglial culture, infected with HIV strain JR-FL
GSM7855215 HIV JR-FL 2 Day 15 time:15 days; cell type:Microglia; treatment:HIV JR-FL Infected iPSC-derived microglial culture, infected with HIV strain JR-FL
GSM7855216 HIV JR-FL 3 Day 15 time:15 days; cell type:Microglia; treatment:HIV JR-FL Infected iPSC-derived microglial culture, infected with HIV strain JR-FL
Platform GPL20301  : Illumina HiSeq 4000 (Homo sapiens)
Literature 38628961  
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xCell Plot
* p<0.05 ** p<0.01