Virus Dataset Detail

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Dataset Information
Accession GSE270130
Status 2024/6/19
Title A nucleosome switch primes Hepatitis B Virus infection [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Chronic hepatitis B virus (HBV) infection is an incurable global health threat responsible for causing liver disease and hepatocellular carcinoma. During the genesis of infection, HBV establishes an independent minichromosome consisting of the viral covalently closed circular DNA (cccDNA) genome and host histones. The viral X gene must be expressed immediately upon infection to induce degradation of the host silencing factor, Smc5/6. However, the relationship between cccDNA chromatinization and X gene transcription remains poorly understood. Establishing a reconstituted viral minichromosome platform, we found that nucleosome occupancy in cccDNA drives X transcription. We corroborated these findings in cells and further showed that the chromatin destabilizing molecule CBL137 inhibits X transcription and HBV infection in hepatocytes. Our results shed light on a long-standing paradox and represent a potential new therapeutic avenue for the treatment of chronic HBV infection.
Samples
GSM ID Sample info Characteristics Description
GSM8335283 HepAD38, DMSO, H3k4me3, rep1 tissue:HepAD38; cell line:Human hepatoma cell line with integrated HBV genome expressed under the control of a tetracycline repressor; cell type:not applicable; genotype:H3K4me3 (Epicypher, #13004) -
GSM8335284 HepAD38, DMSO, H3k4me3, rep2 tissue:HepAD38; cell line:Human hepatoma cell line with integrated HBV genome expressed under the control of a tetracycline repressor; cell type:not applicable; genotype:H3K4me3 (Epicypher, #13004) -
GSM8335285 HepAD38, DMSO, Pol2, rep1 tissue:HepAD38; cell line:Human hepatoma cell line with integrated HBV genome expressed under the control of a tetracycline repressor; cell type:not applicable; genotype:Rpb1 NTD (CST, #14958) -
GSM8335286 HepAD38, DMSO, Pol2, rep2 tissue:HepAD38; cell line:Human hepatoma cell line with integrated HBV genome expressed under the control of a tetracycline repressor; cell type:not applicable; genotype:Rpb1 NTD (CST, #14958) -
GSM8335287 HepAD38, DMSO, Input, rep1 tissue:HepAD38; cell line:Human hepatoma cell line with integrated HBV genome expressed under the control of a tetracycline repressor; cell type:not applicable; genotype:none -
GSM8335288 HepG2, cccDNA-transfected, DMSO, Input, rep1 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:none -
GSM8335289 HepG2, cccDNA-transfected, DMSO, H2A, rep1 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:H2A (CST, #12349) -
GSM8335290 HepG2, cccDNA-transfected, DMSO, H2A, rep2 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:H2A (CST, #12349) -
GSM8335291 HepG2, cccDNA-transfected, DMSO, H2B, rep1 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:H2B (CST, #12364) -
GSM8335292 HepG2, cccDNA-transfected, DMSO, H2B, rep2 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:H2B (CST, #12364) -
GSM8335293 HepG2, cccDNA-transfected, DMSO, H3, rep1 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:H3 (Abcam, #ab1791) -
GSM8335294 HepG2, cccDNA-transfected, DMSO, H3, rep2 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:H3 (Abcam, #ab1791) -
GSM8335295 HepG2, cccDNA-transfected, DMSO, H3K4me3, rep1 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:H3K4me3 (Epicypher, #13004) -
GSM8335296 HepG2, cccDNA-transfected, DMSO, H3K4me3, rep2 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:H3K4me3 (Epicypher, #13004) -
GSM8335297 HepG2, cccDNA-transfected, DMSO, Pol2, rep1 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:Rpb1 NTD (CST, #14958) -
GSM8335298 HepG2, cccDNA-transfected, DMSO, Pol2, rep2 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:Rpb1 NTD (CST, #14958) -
GSM8335299 HepG2, cccDNA-transfected, 500nM CBL137, Input, rep1 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:none -
GSM8335300 HepG2, cccDNA-transfected, 500nM CBL137, H2A, rep1 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:H2A (CST, #12349) -
GSM8335301 HepG2, cccDNA-transfected, 500nM CBL137, H2A, rep2 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:H2A (CST, #12349) -
GSM8335302 HepG2, cccDNA-transfected, 500nM CBL137, H2B, rep1 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:H2B (CST, #12364) -
GSM8335303 HepG2, cccDNA-transfected, 500nM CBL137, H2B, rep2 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:H2B (CST, #12364) -
GSM8335304 HepG2, cccDNA-transfected, 500nM CBL137, H3, rep1 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:H3 (Abcam, #ab1791) -
GSM8335305 HepG2, cccDNA-transfected, 500nM CBL137, H3, rep2 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:H3 (Abcam, #ab1791) -
GSM8335306 HepG2, cccDNA-transfected, 500nM CBL137, H3K4me3, rep1 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:H3K4me3 (Epicypher, #13004) -
GSM8335307 HepG2, cccDNA-transfected, 500nM CBL137, H3K4me3, rep2 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:H3K4me3 (Epicypher, #13004) -
GSM8335308 HepG2, cccDNA-transfected, 500nM CBL137, Pol2, rep1 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:Rpb1 NTD (CST, #14958) -
GSM8335309 HepG2, cccDNA-transfected, 500nM CBL137, Pol2, rep2 tissue:HepG2; cell line:Human hepatoma cells; cell type:HBV cccDNA transfection 24 hours before harvest; genotype:Rpb1 NTD (CST, #14958) -
Platform GPL24676  : Illumina NovaSeq 6000 (Homo sapiens)
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