Accession	GSE270937
Status	2024/7/11
Title	Differential methylation patterns in paternally imprinted gene promoter regions in sperm from hepatitis B virus infected individuals
Organism	Homo sapiens
Experiment type	Methylation profiling by high throughput sequencing
Summary	Background：Hepatitis B virus (HBV) infection poses a substantial threat to human health, impacting not only infected individuals but also potentially exerting adverse effects on the health of their offspring. The underlying mechanisms driving this phenomenon remain elusive. This study aims to shed light on this issue by examining alterations in paternally imprinted genes within sperm. Methods: A cohort of 35 individuals with normal semen analysis, comprising 17 hepatitis B surface antigen (HBsAg)-positive and 18 negative individuals, was recruited. Based on the previous research and the Online Mendelian Inheritance in Man database (OMIM, https://www.omim.org/), targeted promoter methylation sequencing was employed to investigate 28 paternally imprinted genes associated with various diseases. Results: Bioinformatic analyses revealed 42 differentially methylated sites across 29 CpG islands within 19 genes and four differentially methylated CpG islands within four genes. At the gene level, an increase in methylation of DNMT1 and a decrease in methylation of CUL7, PRKAG2, and TP53 were observed. DNA methylation haplotype analysis identified 51 differentially methylated haplotypes within 36 CpG islands across 22 genes. Conclusions: This is the first study to explore the effects of HBV infection on sperm DNA methylation and the potential underlying mechanisms of intergenerational influence of paternal HBV infection.
Samples	GSM ID Sample info Characteristics Description GSM8365381 Sample 1-1_control tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:WT - GSM8365382 Sample 1-2_control tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:WT - GSM8365383 Sample 1-3_control tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:WT - GSM8365384 Sample 1-4_control tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:WT - GSM8365385 Sample 1-5_control tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:WT - GSM8365386 Sample 1-7_control tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:WT - GSM8365387 Sample 1-8_case tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:MT - GSM8365388 Sample 1-9_control tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:WT - GSM8365389 Sample 1-10_control tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:WT - GSM8365390 Sample 1-11_control tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:WT - GSM8365391 Sample 1-12_control tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:WT - GSM8365392 Sample 1-13_control tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:WT - GSM8365402 Sample 2-5_case tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:MT - GSM8365393 Sample 1-14_control tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:WT - GSM8365403 Sample 2-6_case tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:MT - GSM8365394 Sample 1-15_control tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:WT - GSM8365404 Sample 2-7_case tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:MT - GSM8365395 Sample 1-16_control tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:WT - GSM8365405 Sample 2-8_case tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:MT - GSM8365396 Sample 1-17_control tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:WT - GSM8365406 Sample 2-9_case tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:MT - GSM8365397 Sample 1-18_control tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:WT - GSM8365407 Sample 2-10_case tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:MT - GSM8365398 Sample 1-20_case tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:MT - GSM8365408 Sample 2-11_case tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:MT - GSM8365409 Sample 2-12_case tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:MT - GSM8365399 Sample 1-21_control tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:WT - GSM8365410 Sample 2-13_case tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:MT - GSM8365400 Sample 1-22_case tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:MT - GSM8365411 Sample 2-14_case tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:MT - GSM8365401 Sample 2-1_case tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:MT - GSM8365412 Sample 2-15_case tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:MT - GSM8365413 Sample 2-16_case tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:MT - GSM8365414 Sample 2-17_case tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:MT - GSM8365415 Sample 2-18_control tissue:sperm; cell line:haploid cell; cell type:germ cell; genotype:WT -
Platform	GPL24676  : Illumina NovaSeq 6000 (Homo sapiens)
Literature	39090552
Download	Download Methylation Analysis Data