Virus Dataset Detail

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Dataset Information
Accession GSE274780
Status 2024/9/30
Title The glutamate receptor antagonist ifenprodil inhibits hepatitis E virus infection
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Infection with hepatitis E virus (HEV) represents a global problem, with over 20 million people infected annually. No specific antiviral drugs are available for treating HEV infection, necessitating the development of novel targeted therapeutics. Here, we report that the N-methyl-D-aspartate receptor (NMDAR) antagonist ifenprodil, a clinically approved drug used to treat idiopathic pulmonary fibrosis (IPF), is an HEV inhibitor in liver-derived cells. In vitro investigation demonstrates that ifenprodil suppresses viral protein expression in a dose-dependent manner in human hepatoma cells by inhibiting early stages of viral infection. We also found that ifenprodil modulates host cell intrinsic biological processes distinct from virus-induced innate immunity, inhibiting HEV RNA accumulation in primary human hepatocytes. Finally, the inhibitory effect of ifenprodil in vivo was also tested in rabbits challenged with the HEV-3ra CHN-BJ-R14 strain. Fecal virus shedding was below the limit of detection in two animals for both ribavirin-treated and ifenprodil-treated rabbits compared to vehicle-treated control animals. Our data demonstrate that ifenprodil is an effective anti-HEV compound with potential as a therapeutic candidate for the treatment of HEV infection.
Samples
GSM ID Sample info Characteristics Description
GSM8457818 Donor CHM2225, uninfected, DMSO tissue:liver; cell type:primary human hepatocytes; genotype:WT; treatment:DMSO -
GSM8457819 Donor CHM2225, infected, DMSO tissue:liver; agent:hepatitis E virus; cell type:primary human hepatocytes; genotype:WT; treatment:DMSO -
GSM8457820 Donor CHM2225, uninfected, Ifenprodil tissue:liver; cell type:primary human hepatocytes; genotype:WT; treatment:Ifenprodil -
GSM8457821 Donor CHM2225, infected, Ifenprodil tissue:liver; agent:hepatitis E virus; cell type:primary human hepatocytes; genotype:WT; treatment:Ifenprodil -
GSM8457822 Donor CHM2221, uninfected, DMSO tissue:liver; cell type:primary human hepatocytes; genotype:WT; treatment:DMSO -
GSM8457823 Donor CHM2221, infected, DMSO tissue:liver; agent:hepatitis E virus; cell type:primary human hepatocytes; genotype:WT; treatment:DMSO -
GSM8457824 Donor CHM2221, uninfected, Ifenprodil tissue:liver; cell type:primary human hepatocytes; genotype:WT; treatment:Ifenprodil -
GSM8457825 Donor CHM2221, infected, Ifenprodil tissue:liver; agent:hepatitis E virus; cell type:primary human hepatocytes; genotype:WT; treatment:Ifenprodil -
GSM8457826 Donor BHum, uninfected, DMSO tissue:liver; cell type:primary human hepatocytes; genotype:WT; treatment:DMSO -
GSM8457827 Donor BHum, infected, DMSO tissue:liver; agent:hepatitis E virus; cell type:primary human hepatocytes; genotype:WT; treatment:DMSO -
GSM8457828 Donor BHum, uninfected, Ifenprodil tissue:liver; cell type:primary human hepatocytes; genotype:WT; treatment:Ifenprodil -
GSM8457829 Donor BHum, infected, Ifenprodil tissue:liver; agent:hepatitis E virus; cell type:primary human hepatocytes; genotype:WT; treatment:Ifenprodil -
Platform GPL18573  : Illumina NextSeq 500 (Homo sapiens)
Literature 39546567  
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xCell Plot
* p<0.05 ** p<0.01