Virus Dataset Detail

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Dataset Information
Accession GSE281836
Status 2024/11/13
Title Multifaceted roles of RAP80 during the Herpes simplex virus 1 life cycle
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Herpes simplex virus 1 (HSV-1) is a widespread human pathogen that establishes lifelong infections, but understanding precise activity at each infection stage remains challenging. HSV-1 utilizes ubiquitination pathways to modulate cellular factors to facilitate its replication cycle, however, elucidating the cascades that follow host protein degradation and the subsequent impact on the host-virus equilibrium continues to be a complex endeavor. Here we reveal the multifaceted role of the DNA damage response protein RAP80 throughout HSV-1’s infection cycle. In early stages, RAP80 inhibits HSV-1 transcription by directly binding to the viral genome, blocking ICP4 (viral protein) binding to transcription factors. This interaction is regulated by phase separation via an intricate interplay between RAP80 and ICP0/ICP4. As infection progresses, the viral E3 ligase ICP0 degrades RAP80, dissolving phase separation, and allowing the formation of a mature viral replication compartment. In late infection stages, RAP80 is deubiquitinated by the viral deubiquitinase UL36USP, which restores cellular homeostasis and promotes HSV-1 survival. This process involves modulating the R-loop-cGAS-apoptosis pathway. These findings underscore the dynamic interplay between viral and host factors and the complex mechanisms used by HSV-1 to subvert host defenses, offering practical implications for the future development of antiviral strategies.
Samples
GSM ID Sample info Characteristics Description
GSM8630806 HeLa, RAP80, ChIP, 3h cell line:HeLa; cell type:Cervical cancer epithelial cells; infection:HSV-1; genotype:anti-RAP80 (ab70822, Abcam); time:3h -
GSM8630807 HeLa, RAP80, ChIP, 20h cell line:HeLa; cell type:Cervical cancer epithelial cells; infection:HSV-1; genotype:anti-RAP80 (ab70822, Abcam); time:20h -
Platform GPL24676  : Illumina NovaSeq 6000 (Homo sapiens)
Literature   
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