Accession	GSE30736
Status	2011/11/6
Title	Genome-wide binding map of the HIV Tat protein to the human genome (ChIP-chip)
Organism	Homo sapiens
Experiment type	Genome binding/occupancy profiling by genome tiling array
Summary	The HIV-1 Trans-Activator of Transcription (Tat) protein binds to multiple host cellular factors and greatly enhances the level of transcription of the HIV genome. Here, we report the genome-wide binding map of Tat to the human genome in Jurkat T cells (Jurkat-Tat cells) using chromatin immunoprecipitation combined with next-generation sequencing. cDNA microarray was used to monitor gene expression changes between Jurkat and Jurkat-Tat cells. Additionally, we compared distribution of H3K9ac near gene promoters between Jurkat and Jurkat-Tat cells using ChIP-chip method and hybridized onto Agilent promoter array. Our data reveal that Tat’s interaction with the host genome is more extensive than previously thought, with potentially important implications for the viral life cycle.
Samples	GSM ID Sample info Characteristics Description GSM762829 Jurkat Tat cells vs Jurkat cells H3K9ac ChIP-chip expression:HIV Tat protein; chip antibody:H3K9ac; cell line:Jurkat; chip antibody manufacturer:Upstate; chip antibody catalog #:07-352; chip antibody manufacturer:Upstate; chip antibody catalog #:07-352 - GSM762829 Jurkat Tat cells vs Jurkat cells H3K9ac ChIP-chip expression:control; chip antibody:H3K9ac; cell line:Jurkat -
Platform	GPL7032  : Human Promoter ChIP-on-chip Microarray - custom annotation
Literature	22073215