Accession	GSE30738
Status	2011/11/6
Title	Genome-wide binding map of the HIV Tat protein to the human genome (ChIP-Seq)
Organism	Homo sapiens
Experiment type	Genome binding/occupancy profiling by high throughput sequencing
Summary	The HIV-1 Trans-Activator of Transcription (Tat) protein binds to multiple host cellular factors and greatly enhances the level of transcription of the HIV genome. Here, we report the genome-wide binding map of Tat to the human genome in Jurkat T cells (Jurkat-Tat cells) using chromatin immunoprecipitation combined with next-generation sequencing. cDNA microarray was used to monitor gene expression changes between Jurkat and Jurkat-Tat cells. Additionally, we compared distribution of H3K9ac near gene promoters between Jurkat and Jurkat-Tat cells using ChIP-chip method and hybridized onto Agilent promoter array. Our data reveal that Tat’s interaction with the host genome is more extensive than previously thought, with potentially important implications for the viral life cycle.
Samples	GSM ID Sample info Characteristics Description GSM762834 input DNA expression:HIV Tat protein; chip antibody:none; cell line:Jurkat input DNA GSM762835 Tat ChIP-seq expression:HIV Tat protein; chip antibody:Tat; cell line:Jurkat; chip antibody manufacturer:Abcam; chip antibody catalog #:ab43014 chromatin ChIPed against HIV tat
Platform	GPL9115  : Illumina Genome Analyzer II (Homo sapiens)
Literature	22073215
Download	Download Genome Binding/Occupancy Profiling Analysis Data