Accession	GSE35462
Status	2012/8/1
Title	Genome-wide analysis of histone methylation reveals chromatin state-based regulation of host cellular gene expression induced by hepatitis B viruses (ChIP-Seq dataset)
Organism	Homo sapiens
Experiment type	Genome binding/occupancy profiling by high throughput sequencing
Summary	Hepatitis B virus (HBV) is a hepatotropic virus that can regulate many host cellular gene expressions participating in the HBV life cycle, liver inflammation and hepatocellular injury. However, the underlying mechanism of differential gene expression is not understood. We report here a genome-wide analysis of histone methylation on two histone H3 lysine residues (H3K4me3 and H3K27me3) and gene expression profiles in HepG2 and HepG2.2.15 cells. We found that specific correlation exists between gene expression and the amounts of H3K4me3 (positive correlation) and H3K27me3 (negative correlation) across the gene body. These correlations displayed three distinct modes (repressive, active and poised), reflecting different functions of these genes in the HBV life cycle, liver inflammation and hepatocellular injury. Furthermore, a permissive chromatin state of each gene was established by a combination of different histone modifications. Our findings reveal a complex regulation by histone methylation in differential gene expression and suggest that histone methylation may be responsible for the HBV life cycle, liver inflammation and hepatocellular injury induced by HBV.
Samples	GSM ID Sample info Characteristics Description GSM868998 HepG2.H3K27me3_ChIP-Seq cell line:HepG2; cell type:human hepatocellular liver carcinoma cells; chip antibody:anti-H3K27me3; antibody vendor:Abcam; antibody catalog #:ab6002; antibody lot #:GR39353-1 - GSM868999 HepG2.2.15.H3K4me3_ChIP-Seq cell line:HepG2.2.15; cell type:human hepatocellular liver carcinoma cells; chip antibody:anti-H3K4me3; antibody vendor:Abcam; antibody catalog #:ab8580; antibody lot #:1016899 - GSM869000 HepG2.2.15.H3K27me3_ChIP-Seq cell line:HepG2.2.15; cell type:human hepatocellular liver carcinoma cells; chip antibody:anti-H3K27me3; antibody vendor:Abcam; antibody catalog #:ab6002; antibody lot #:GR39353-1 - GSM868997 HepG2.H3K4me3_ChIP-Seq cell line:HepG2; cell type:human hepatocellular liver carcinoma cells; chip antibody:anti-H3K4me3; antibody vendor:Abcam; antibody catalog #:ab8580; antibody lot #:1016899 -
Platform	GPL11154  : Illumina HiSeq 2000 (Homo sapiens)
Literature	24067902