Virus Dataset Detail

> Detail information for each sample

Dataset Information
Accession GSE51993
Status 2013/11/2
Title microRNA differential regulations gradually mediate genomic instability during cervical intraepithelial neoplasia progression
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array;Expression profiling by array
Summary In most cases human papillomavirus (HPV) infections are cleared from the cervical cells by the immune system itself, but in a few cases, where there is persistent HPV infection, it can lead to cervical intraepithelial neoplasia (CIN) progression and ultimately invasive cervical carcinoma. The cytopathic effect is in general accompanied by chronic inflammation, which produces inflammation cytokines that contribute to DNA damage, and at the same time, aberrations occurred in the host DNA repair mechanisms, thus lead to HPV genomic integration into the host cells which propels cell immortalization. In this study, we reported the genome-wide expression profiles of both microRNAs (miRNAs) and mRNAs from 24 cervical samples with consecutive stages of normal, CIN I (mild dysplasia) and CIN III (severe dysplasia and carcinoma in situ), and presented the SIG++ algorithm which is founded on the evolution process of intermolecular regulation change during disease progression, to identify the significant change of miRNA-mRNA regulations rather than the expression change, across different disease stages, thereupon elucidating the molecular mechanisms of increasing host genomic instability as disease progresses. As reconstructing miRNA differential networks, we found that at each stage of CIN, there respectively exists specific miRNA regulations mediating chronic inflammation persistence, genome instability and cell survival, which coordinately carrys out the integration of HPV genomes into the host cell genomes, and finally results in cell immortalization. Beyond the specific implications for cervical carcinogenesis, this work establishes a new framework for studying the biology of miRNAs in pathogenesis from the perspective of miRNA differential regulation, and helps ensure the comprehensiveness of miRNA-mediated genetic regulatory pathways.
Samples
GSM ID Sample info Characteristics Description
GSM1256899 total microRNAs in a clinical sample at normal stage_N24+ gender:female; tissue:cervix; disease stage:normal (adjacent tissue of early lesions); molecule subtype:miRNA SAMPLE mi_N24+
GSM1256900 total microRNAs in a clinical sample at normal stage_N180 gender:female; tissue:cervix; disease stage:normal (adjacent tissue of early lesions); molecule subtype:miRNA SAMPLE mi_N180
GSM1256881 total microRNAs in a clinical sample at CIN III stage_C33 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) III; molecule subtype:miRNA SAMPLE mi_C33
GSM1256901 total microRNAs in a clinical sample at normal stage_N23+ gender:female; tissue:cervix; disease stage:normal (adjacent tissue of early lesions); molecule subtype:miRNA SAMPLE mi_N23+
GSM1256882 total microRNAs in a clinical sample at CIN III stage_C34 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) III; molecule subtype:miRNA SAMPLE mi_C34
GSM1256902 total microRNAs in a clinical sample at normal stage_N90 gender:female; tissue:cervix; disease stage:normal (adjacent tissue of early lesions); molecule subtype:miRNA SAMPLE mi_N90
GSM1256903 total microRNAs in a clinical sample at normal stage_N30+ gender:female; tissue:cervix; disease stage:normal (adjacent tissue of early lesions); molecule subtype:miRNA SAMPLE mi_N30+
GSM1256883 total microRNAs in a clinical sample at CIN III stage_C54 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) III; molecule subtype:miRNA SAMPLE mi_C54
GSM1256904 total microRNAs in a clinical sample at normal stage_N50+ gender:female; tissue:cervix; disease stage:normal (adjacent tissue of early lesions); molecule subtype:miRNA SAMPLE mi_N50+
GSM1256905 total mRNAs in a clinical sample at CIN III stage_C33 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) III SAMPLE m_C33
GSM1256906 total mRNAs in a clinical sample at CIN III stage_C34 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) III SAMPLE m_C34
GSM1256907 total mRNAs in a clinical sample at CIN III stage_C54 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) III SAMPLE m_C54
GSM1256908 total mRNAs in a clinical sample at CIN III stage_C31 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) III SAMPLE m_C31
GSM1256909 total mRNAs in a clinical sample at CIN III stage_C27 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) III SAMPLE m_C27
GSM1256910 total mRNAs in a clinical sample at CIN III stage_C25 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) III SAMPLE m_C25
GSM1256911 total mRNAs in a clinical sample at CIN III stage_C68 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) III SAMPLE m_C68
GSM1256912 total mRNAs in a clinical sample at CIN III stage_C71 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) III SAMPLE m_C71
GSM1256913 total mRNAs in a clinical sample at CIN I stage_C35 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) I SAMPLE m_C35
GSM1256914 total mRNAs in a clinical sample at CIN I stage_N21 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) I SAMPLE m_N21
GSM1256915 total mRNAs in a clinical sample at CIN I stage_C57 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) I SAMPLE m_C57
GSM1256916 total mRNAs in a clinical sample at CIN I stage_C58 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) I SAMPLE m_C58
GSM1256917 total mRNAs in a clinical sample at CIN I stage_N40 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) I SAMPLE m_N40
GSM1256918 total mRNAs in a clinical sample at CIN I stage_N44 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) I SAMPLE m_N44
GSM1256919 total mRNAs in a clinical sample at CIN I stage_N46 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) I SAMPLE m_N46
GSM1256920 total mRNAs in a clinical sample at CIN I stage_N48 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) I SAMPLE m_N48
GSM1256884 total microRNAs in a clinical sample at CIN III stage_C31 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) III; molecule subtype:miRNA SAMPLE mi_C31
GSM1256921 total mRNAs in a clinical sample at CIN I stage_C59 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) I SAMPLE m_C59
GSM1256885 total microRNAs in a clinical sample at CIN III stage_C27 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) III; molecule subtype:miRNA SAMPLE mi_C27
GSM1256922 total mRNAs in a clinical sample at normal stage_N5+ gender:female; tissue:cervix; disease stage:normal (adjacent tissue of early lesions) SAMPLE m_N5+
GSM1256886 total microRNAs in a clinical sample at CIN III stage_C25 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) III; molecule subtype:miRNA SAMPLE mi_C25
GSM1256923 total mRNAs in a clinical sample at normal stage_N24+ gender:female; tissue:cervix; disease stage:normal (adjacent tissue of early lesions) SAMPLE m_N24+
GSM1256887 total microRNAs in a clinical sample at CIN III stage_C68 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) III; molecule subtype:miRNA SAMPLE mi_C68
GSM1256924 total mRNAs in a clinical sample at normal stage_N180 gender:female; tissue:cervix; disease stage:normal (adjacent tissue of early lesions) SAMPLE m_N180
GSM1256925 total mRNAs in a clinical sample at normal stage_N23+ gender:female; tissue:cervix; disease stage:normal (adjacent tissue of early lesions) SAMPLE m_N23+
GSM1256926 total mRNAs in a clinical sample at normal stage_N90 gender:female; tissue:cervix; disease stage:normal (adjacent tissue of early lesions) SAMPLE m_N90
GSM1256927 total mRNAs in a clinical sample at normal stage_N30+ gender:female; tissue:cervix; disease stage:normal (adjacent tissue of early lesions) SAMPLE m_N30+
GSM1256888 total microRNAs in a clinical sample at CIN III stage_C71 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) III; molecule subtype:miRNA SAMPLE mi_C71
GSM1256928 total mRNAs in a clinical sample at normal stage_N50+ gender:female; tissue:cervix; disease stage:normal (adjacent tissue of early lesions) SAMPLE m_N50+
GSM1256889 total microRNAs in a clinical sample at CIN I stage_C35 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) I; molecule subtype:miRNA SAMPLE mi_C35
GSM1256890 total microRNAs in a clinical sample at CIN I stage_N21 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) I; molecule subtype:miRNA SAMPLE mi_N21
GSM1256891 total microRNAs in a clinical sample at CIN I stage_C57 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) I; molecule subtype:miRNA SAMPLE mi_C57
GSM1256892 total microRNAs in a clinical sample at CIN I stage_C58 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) I; molecule subtype:miRNA SAMPLE mi_C58
GSM1256893 total microRNAs in a clinical sample at CIN I stage_N40 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) I; molecule subtype:miRNA SAMPLE mi_N40
GSM1256894 total microRNAs in a clinical sample at CIN I stage_N44 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) I; molecule subtype:miRNA SAMPLE mi_N44
GSM1256895 total microRNAs in a clinical sample at CIN I stage_N46 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) I; molecule subtype:miRNA SAMPLE mi_N46
GSM1256896 total microRNAs in a clinical sample at CIN I stage_N48 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) I; molecule subtype:miRNA SAMPLE mi_N48
GSM1256897 total microRNAs in a clinical sample at CIN I stage_C59 gender:female; tissue:cervix; disease stage:cervical intraepithelial neoplasia (CIN) I; molecule subtype:miRNA SAMPLE mi_C59
GSM1256898 total microRNAs in a clinical sample at normal stage_N5+ gender:female; tissue:cervix; disease stage:normal (adjacent tissue of early lesions); molecule subtype:miRNA SAMPLE mi_N5+
Platform GPL8179  GPL10558  : Illumina Human v2 MicroRNA expression beadchip;Illumina HumanHT-12 V4.0 expression beadchip
Literature   
Download Download xCell Data  Download Expression Analysis Data  
xCell Plot
* p<0.05 ** p<0.01