Virus Dataset Detail

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Dataset Information
Accession GSE80131
Status 2016/7/11
Title Topoisomerase 1 inhibition suppresses inflammatory genes and protects from death by inflammation (RNA-Seq)
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The host innate immune response is the first line of defense against pathogens and is orchestrated by the concerted expression of genes induced by microbial stimuli. Deregulated expression of these genes is linked to the initiation and progression of numerous diseases associated with exacerbated inflammation. Here, we identify Topoisomerase 1 (Top1) as a critical positive regulator of RNA polymerase II (RNAPII) transcriptional activity at pathogen-induced genes. Notably, depletion or chemical inhibition of Top1 suppresses the host response against replicating Influenza and Ebola viruses as well as bacterial products. As a result, therapeutic pharmacological inhibition of Top1 protects mice from death in experimental models of chemical- and pathogen-induced lethal inflammation. Our results indicate that Top1 inhibition could be used as therapy against life threatening infections characterized by an acutely exacerbated immune response.
Samples
GSM ID Sample info Characteristics Description
GSM2113344 Mock Campothecin cell type:THP-1; treatment:Campothecin RNA sequencing
GSM2113345 Mock DMSO cell type:THP-1; treatment:DMSO RNA sequencing
GSM2113346 Mutated Ebola Campothecin cell type:THP-1; treatment:Campothecin RNA sequencing
GSM2113347 Mutated Ebola DMSO cell type:THP-1; treatment:DMSO RNA sequencing
GSM2113348 Wild-type Ebola Campothecin cell type:THP-1; treatment:Campothecin RNA sequencing
GSM2113349 Wild-type Ebola DMSO cell type:THP-1; treatment:DMSO RNA sequencing
Platform GPL16791  : Illumina HiSeq 2500 (Homo sapiens)
Literature 27127234  
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xCell Plot
* p<0.05 ** p<0.01