Accession	GSE85724
Status	2016/8/18
Title	m6A sequencing of HIV viral genomic RNA and infected host cell samples
Organism	Homo sapiens
Experiment type	Expression profiling by high throughput sequencing
Summary	The internal N6-methyladenosine (m6A) methylation of eukaryotic nuclear RNA controls post-transcriptional gene expression, which is regulated by methyltransferases (writers), demethylases (erasers), and m6A-binding proteins (readers) in cells. The YTH domain family proteins (YTHDF1–3) bind to m6A-modified cellular RNAs and affect RNA metabolism and processing. Here, we show that YTHDF1–3 proteins recognize m6A-modified HIV-1 RNA and inhibit HIV-1 infection in cell lines and primary CD4+ T-cells. We further mapped the YTHDF1–3 binding sites in HIV-1 RNA from infected cells. We found that the overexpression of YTHDF proteins in cells inhibited HIV-1 infection mainly by decreasing HIV-1 reverse transcription, while knockdown of YTHDF1–3 in cells had the opposite effects. Moreover, silencing the m6A writers decreased HIV-1 Gag protein expression in virus-producing cells, while silencing the m6A erasers increased Gag expression. Our findings suggest an important role of m6A modification of HIV-1 RNA in viral infection and HIV-1 protein synthesis.
Samples	GSM ID Sample info Characteristics Description GSM2283207 HIV infect 2 input cell type:Jurkat; infected with:HIV-1; molecule subtype:mRNA - GSM2283208 HIV Tcell cont input cell type:Primary CD4+ T; infected with:none (control); molecule subtype:mRNA - GSM2283209 HIV Tcell infect input cell type:Primary CD4+ T; infected with:HIV-1; molecule subtype:mRNA - GSM2283210 HIV Cont 1 IP cell type:Jurkat; infected with:none (control); molecule subtype:mRNA processed data file: HIV.Cont.bed GSM2283211 HIV Cont 2 IP cell type:Jurkat; infected with:none (control); molecule subtype:mRNA processed data file: HIV.Cont.bed GSM2283212 HIV infect 1 IP cell type:Jurkat; infected with:HIV-1; molecule subtype:mRNA processed data file: HIV.Infect.bed GSM2283213 HIV infect 2 IP cell type:Jurkat; infected with:HIV-1; molecule subtype:mRNA processed data file: HIV.Infect.bed GSM2283214 HIV Tcell cont IP cell type:Primary CD4+ T; infected with:none (control); molecule subtype:mRNA - GSM2283215 HIV Tcell infect IP cell type:Primary CD4+ T; infected with:HIV-1; molecule subtype:mRNA - GSM2283216 Y1-CLIP cell type:HeLa; infected with:HIV-1; molecule subtype:protein-bound RNA - GSM2283217 Y2-CLIP cell type:HeLa; infected with:HIV-1; molecule subtype:protein-bound RNA - GSM2283218 Y3-CLIP cell type:HeLa; infected with:HIV-1; molecule subtype:protein-bound RNA - GSM2283204 HIV Cont 1 input cell type:Jurkat; infected with:none (control); molecule subtype:mRNA - GSM2283205 HIV Cont 2 input cell type:Jurkat; infected with:none (control); molecule subtype:mRNA - GSM2283206 HIV infect 1 input cell type:Jurkat; infected with:HIV-1; molecule subtype:mRNA -
Platform	GPL16791  : Illumina HiSeq 2500 (Homo sapiens)
Literature	27371828  28901284