Accession	GSE98057
Status	2017/4/22
Title	Identification of symmetric methylation of histone H4 arginine 3 on KSHV genome (TREx Und 12h 24hInd ChIP-Seq)
Organism	Human gammaherpesvirus 8
Experiment type	Genome binding/occupancy profiling by high throughput sequencing
Summary	The objective of this study was to identify the symmetric methylation sites on KSHV genome during latency and lytic reactivation
Samples	GSM ID Sample info Characteristics Description GSM2586472 Und Input tissue:KSHV infected Body Cavity Based Lymphoma Cells; genotype/variation:RTA protein is under tet-inducible promoter; antibody:none (input) DNA extracted from the sonicated chromatin GSM2586473 Und PRMT5 ChIP tissue:KSHV infected Body Cavity Based Lymphoma Cells; genotype/variation:RTA protein is under tet-inducible promoter; antibody:PRMT5 Antibody: Active Motif #61001 PRMT5 ChIP GSM2586474 Und COPR5 ChIP tissue:KSHV infected Body Cavity Based Lymphoma Cells; genotype/variation:RTA protein is under tet-inducible promoter; antibody:COPR5 Antibody: Novus Biologicals #NBP2-30884 COPR5 ChIP GSM2586475 12hInd PRMT5 ChIP tissue:KSHV infected Body Cavity Based Lymphoma Cells; genotype/variation:RTA protein is under tet-inducible promoter; antibody:PRMT5 Antibody: Active Motif #61001 PRMT5 ChIP GSM2586476 12hInd ORF59 ChIP tissue:KSHV infected Body Cavity Based Lymphoma Cells; genotype/variation:RTA protein is under tet-inducible promoter; antibody:ORF59 Antibody: Custom synthesized for our lab - anti-KSHV-ORF59 antibody from GenScript ORF59 ChIP GSM2586477 24hInd Input tissue:KSHV infected Body Cavity Based Lymphoma Cells; genotype/variation:RTA protein is under tet-inducible promoter; antibody:none (input) DNA extracted from the sonicated chromatin GSM2586478 24hInd IgG ChIP tissue:KSHV infected Body Cavity Based Lymphoma Cells; genotype/variation:RTA protein is under tet-inducible promoter; antibody:Control IgG Antibody: Cell Signaling Technology, catalog #2729, lot# 7 Control IgG ChIP GSM2586479 24hInd PRMT5 ChIP tissue:KSHV infected Body Cavity Based Lymphoma Cells; genotype/variation:RTA protein is under tet-inducible promoter; antibody:PRMT5 Antibody: Active Motif #61001 PRMT5 ChIP GSM2586480 24hInd COPR5 ChIP tissue:KSHV infected Body Cavity Based Lymphoma Cells; genotype/variation:RTA protein is under tet-inducible promoter; antibody:COPR5 Antibody: Novus Biologicals #NBP2-30884 COPR5 ChIP GSM2586481 24hInd ORF59 ChIP tissue:KSHV infected Body Cavity Based Lymphoma Cells; genotype/variation:RTA protein is under tet-inducible promoter; antibody:ORF59 Antibody: Custom synthesized for our lab - anti-KSHV-ORF59 antibody from GenScript ORF59 ChIP
Platform	GPL23366  : Illumina NextSeq 500 (Human gammaherpesvirus 8)
Literature	28678843