Accession	GSE98087
Status	2017/4/24
Title	Identification of symmetric methylation of histone H4 arginine 3 on KSHV genome (iSLKBac16WT ChIP-Seq)
Organism	Human gammaherpesvirus 8
Experiment type	Genome binding/occupancy profiling by high throughput sequencing
Summary	The objective of this study was to identify the symmetric methylation sites on KSHV genome during latency and lytic reactivation
Samples	GSM ID Sample info Characteristics Description GSM2586923 24hInd Input (iSLK-Bac16WT cells) cell type: KSHV positive iSLK endothelial cells derived from gingival KS lesion;genotype/variation: RTA protein is under tet-inducible promoter;treatment: induced with doxycycline for 24h to trigger lytic reactivation;chip antibody: none DNA extracted from the sonicated chromatin GSM2586924 24hInd ORF59 ChIP (iSLK-Bac16WT cells) cell type: KSHV positive iSLK endothelial cells derived from gingival KS lesion;genotype/variation: RTA protein is under tet-inducible promoter;treatment: induced with doxycycline for 24h to trigger lytic reactivation;chip antibody: ORF59 (Custom synthesized for our lab - anti-KSHV-ORF59 antibody from GenScript) ORF59 ChIP GSM2586925 24hInd Control IgG ChIP (iSLK-Bac16WT cells) cell type: KSHV positive iSLK endothelial cells derived from gingival KS lesion;genotype/variation: RTA protein is under tet-inducible promoter;treatment: induced with doxycycline for 24h to trigger lytic reactivation;chip antibody: IgG (Cell Signaling Technology, catalog #2729, lot# 7) control IgG ChIP GSM2586926 24hInd + 0.5mM PFA ORF59 ChIP (iSLK-Bac16WT cells) cell type: KSHV positive iSLK endothelial cells derived from gingival KS lesion;genotype/variation: RTA protein is under tet-inducible promoter;treatment: induced with doxycycline for 24h to trigger lytic reactivation;agent: PFA;chip antibody: ORF59 (Custom synthesized for our lab - anti-KSHV-ORF59 antibody from GenScript) ORF59 ChIP
Platform	GPL23366  : Illumina NextSeq 500 (Human gammaherpesvirus 8)
Literature	28678843